AB252538

Anti-Kv4.3/KCND3 antibody [K75/41]

BOND RX™ Validated
Recombinant
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Mouse Recombinant Monoclonal Kv4.3/KCND3 antibody. Suitable for IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

A-type voltage-gated potassium channel KCND3, Potassium voltage-gated channel subfamily D member 3, Voltage-gated potassium channel subunit Kv4.3, Kcnd3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (AB252538)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (AB252538)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the mouse cerebrum. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (AB252538)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on the mouse skeletal muscle. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (AB252538)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on the rat skeletal muscle. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (AB252538)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Kv4.3/KCND3 with ab252538 at 1/200 dilution, followed by secondary LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the rat cerebrum. The section was incubated with ab252538 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Carrier free

Anti-Kv4.3/KCND3 antibody [K75/41] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

K75/41

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kv4.3 also known as KCND3 is a voltage-gated potassium channel subunit with an important role in membrane repolarization. This protein with a mass of approximately 71 kDa is extensively expressed in the heart and brain. Kv4.3 contributes to the formation of transient outward potassium currents (Ito) that are essential for shaping the action potentials in neurons and cardiac myocytes. These currents help in the rapid repolarization phase that restores the resting membrane potential after depolarization.

Biological function summary

Kv4.3 influences electrical signaling and cellular excitability in the cardiovascular and nervous systems. It often forms complexes with auxiliary subunits such as KChIP1-4 (Kv channel-interacting proteins) which modulate its current properties and localization. In neurons Kv4.3 helps regulate firing patterns while in cardiac tissue it affects heart rate and contractility. The presence of these complexes ensures precise control of channel function fitting the specific needs of tissue type and physiological condition.

Pathways

Kv4.3 integrates into critical signaling networks that involve ion homeostasis and action potential propagation. It belongs to the pathway that manages membrane potential dynamics interacting with proteins like Kv4.2 and Kv2.1 as part of a broader set of voltage-gated potassium channels. This network plays a role in the Wnt signaling pathway impacting cellular processes such as cell cycle regulation and differentiation.

Kv4.3 has been linked with cardiac arrhythmias and neurological disorders. Altered Kv4.3 functionality can lead to improper cardiac electrical activity resulting in arrhythmic events such as Long QT Syndrome. In the brain aberrations in Kv4.3 channel activity can correlate with epilepsy where disrupted ion channel regulation triggers excessive neuronal firing. Its involvement in these conditions highlights its participation in concert with other related proteins like SCN5A in cardiac tissues and CACNA1H in neuronal contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pore-forming (alpha) subunit of voltage-gated A-type potassium channels that mediates transmembrane potassium transport in excitable membranes, in brain and heart (By similarity). In cardiomyocytes, may generate the transient outward potassium current I(To). In neurons, may conduct the transient subthreshold somatodendritic A-type potassium current (ISA) (By similarity). Kinetics properties are characterized by fast activation at subthreshold membrane potentials, rapid inactivation, and quick recovery from inactivation. Channel properties are modulated by interactions with regulatory subunits. Interaction with the regulatory subunits KCNIP1 or KCNIP2 modulates the channel gating kinetics namely channel activation and inactivation kinetics and rate of recovery from inactivation. Likewise, interaction with DPP6 modulates the channel gating kinetics namely channel activation and inactivation kinetics (By similarity).

See full target information Kcnd3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kv4.3/KCND3 antibody [K75/41]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (AB252538)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (AB252538)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (AB252538)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.3/KCND3 antibody [K75/41] (AB252538)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise