AB233529

Anti-kynurenine 3-monooxygenase antibody

Name: Anti-kynurenine 3-monooxygenase antibody (ab233529) | Abcam
Brand: Abcam Limited
SKU: AB233529
Price: 525 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal kynurenine 3-monooxygenase antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Rat, Rat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human kynurenine 3-monooxygenase aa 1-300.

View Alternative Names

Kynurenine 3-monooxygenase, Kynurenine 3-hydroxylase, KMO

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-kynurenine 3-monooxygenase antibody (AB233529)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-kynurenine 3-monooxygenase antibody (AB233529)

Formalin-fixed, paraffin-embedded rat heart tissue stained for kynurenine 3-monooxygenase using ab233529 at 20μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-kynurenine 3-monooxygenase antibody (AB233529)

Formalin-fixed, paraffin-embedded rat skin tissue stained for kynurenine 3-monooxygenase using ab233529 at 20μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-kynurenine 3-monooxygenase antibody (AB233529)

Formalin-fixed, paraffin-embedded rat liver tissue stained for kynurenine 3-monooxygenase using ab233529 at 20μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-kynurenine 3-monooxygenase antibody (AB233529)

All lanes:

Western blot - Anti-kynurenine 3-monooxygenase antibody (ab233529) at 1 µg/mL

All lanes:

Recombinant kynurenine 3-monooxygenase protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution

Predicted band size: 122 kDa,56 kDa

true

Supplier Data

Western blot - Anti-kynurenine 3-monooxygenase antibody (AB233529)

All lanes:

Western blot - Anti-kynurenine 3-monooxygenase antibody (ab233529) at 1 µg/mL

Lane 1:

Rat kidney tissue lysate

Lane 2:

Rat spleen tissue lysate

Lane 3:

Rat liver tissue lysate

Lane 4:

Rat heart tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution

Predicted band size: 56 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human kynurenine 3-monooxygenase aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O15229

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Recombinant fragment - Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kynurenine 3-monooxygenase (KMO) also known as kynurenine 3-hydroxylase is an enzyme that plays a mechanical role in the kynurenine pathway. This enzyme catalyzes the conversion of L-kynurenine to 3-hydroxykynurenine. KMO has a molecular mass of approximately 55 kDa. It is primarily expressed in the liver and kidney tissues but can also be found in other organs at lower levels. The structure of KMO contains a flavin adenine dinucleotide (FAD) binding domain which is essential for its oxidoreductase activity.

Biological function summary

KMO participates in the degradation of tryptophan significantly impacting the biosynthesis of nicotinamide adenine dinucleotide (NAD+). KMO is not known to be part of any protein complexes but its enzymatic activity is important for the production of downstream metabolites that influence various physiological processes. This activity affects immune response neurogenesis and energy metabolism highlighting its role in maintaining metabolic balance.

Pathways

KMO is an integral component of the kynurenine pathway which is critical for tryptophan catabolism. It associates closely with other enzymes like kynureninase and kynurenine aminotransferase. KMO exerts its function by regulating the levels of neuroactive and immunoactive metabolites in the brain and peripheral tissues. Through its role in the kynurenine pathway KMO influences cellular signaling and energy production connecting to broader metabolic pathways.

KMO plays a significant role in neurodegenerative diseases such as Alzheimer's and Huntington's disease. Altered KMO activity leads to an imbalance of kynurenine pathway metabolites impacting neuronal health. The enzyme's dysfunction also associates with psychiatric disorders like schizophrenia. The relationship between KMO and other kynurenine pathway enzymes like kynurenine aminotransferase suggests that modulating its activity could present therapeutic opportunities for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn) (PubMed : 23575632, PubMed : 26752518, PubMed : 28604669, PubMed : 29208702, PubMed : 29429898). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract (Probable).

See full target information kynurenine 3-monooxygenase

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2406026 PubMed39494721

2024

Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Ouyang,Yaling Li,Haoming Wang,Xiangyang Liu,Xiaoli Tan,Genyuan Xie,Junfa Zeng,Gaofeng Zeng,Qiong Luo,Hong Zhou,Siming Chen,Kai Hou,Jinren Fang,Xia Zhang,Linlin Zhou,Yukun Li,Anbo Gao

International journal of molecular sciences 25: PubMed39000602

2024

Inhibition of Indoleamine 2,3-Dioxygenase Exerts Antidepressant-like Effects through Distinct Pathways in Prelimbic and Infralimbic Cortices in Rats under Intracerebroventricular Injection with Streptozotocin.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Qin,Xiao Hu,Hui-Ling Zhao,Nurhumar Kurban,Xi Chen,Jing-Kun Yi,Yuan Zhang,Su-Ying Cui,Yong-He Zhang

Frontiers in genetics 13:860067 PubMed36199579

2022

Transcriptome profiles of fatty acid metabolism-related genes and immune infiltrates identify hot tumors for immunotherapy in cutaneous melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxian Dong,Zirui Zhao,Maijimi Simayi,Chufen Chen,Zhongye Xu,Dongming Lv,Bing Tang

Science (New York, N.Y.) 373: PubMed34437091

2021

Enterically derived high-density lipoprotein restrains liver injury through the portal vein.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Hyun Han,Emily J Onufer,Li-Hao Huang,Robert W Sprung,W Sean Davidson,Rafael S Czepielewski,Mary Wohltmann,Mary G Sorci-Thomas,Brad W Warner,Gwendalyn J Randolph

Transplant immunology 68:101393 PubMed33838298

2021

Immutol regulates CD4Tregs, CD8Tregs and pDCs via IDO signaling pathway to induce immune tolerance in rat heart allograft transplant.

Applications

Unspecified application

Species

Unspecified reactive species

Long Yang,Jun Ma,Qiang He,Xianliang Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-kynurenine 3-monooxygenase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-kynurenine 3-monooxygenase antibody (AB233529)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-kynurenine 3-monooxygenase antibody (AB233529)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-kynurenine 3-monooxygenase antibody (AB233529)

Western blot - Anti-kynurenine 3-monooxygenase antibody (AB233529)

Western blot - Anti-kynurenine 3-monooxygenase antibody (AB233529)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise