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AB236741

Anti-Kyphoscoliosis peptidase antibody

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(1 Publication)

Rabbit Polyclonal Kyphoscoliosis peptidase antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KY aa 1-200.

View Alternative Names

Kyphoscoliosis peptidase, KY

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Kyphoscoliosis peptidase antibody (AB236741)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Kyphoscoliosis peptidase antibody (AB236741)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Kyphoscoliosis peptidase (green) using ab236741 at 1/133 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counterstain is DAPI (blue).

The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kyphoscoliosis peptidase antibody (AB236741)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kyphoscoliosis peptidase antibody (AB236741)

Paraffin-embedded human skeletal muscle tissue stained for Kyphoscoliosis peptidase using ab236741 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-Kyphoscoliosis peptidase antibody (AB236741)
  • WB

Supplier Data

Western blot - Anti-Kyphoscoliosis peptidase antibody (AB236741)

All lanes:

Western blot - Anti-Kyphoscoliosis peptidase antibody (ab236741) at 1/500 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KY aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q8NBH2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kyphoscoliosis peptidase also known as ADAMTS-like protein 2 is a metalloproteinase. This protein family includes enzymes that break down extracellular matrix components. Kyphoscoliosis peptidase has a mass of around 130 kDa and is involved in the processing of several structural molecules. It is primarily expressed in tissues like skin tendon and cartilage indicating its main physiological roles. Its expression in these dense connective tissues suggests it plays a role in structural integrity and remodeling of the extracellular matrix.
Biological function summary

Kyphoscoliosis peptidase contributes to maintaining the elastic properties of connective tissues. It assists in the proper organization and assembly of collagen fibrils by processing precursor molecules part of its enzymatic function. This protein may interact with other components of the extracellular matrix such as fibronectin or proteoglycans suggesting its involvement in a larger molecular complex. This activity is important in tissues undergoing constant mechanical stress and requiring elasticity.

Pathways

Kyphoscoliosis peptidase participates in the regulation of collagen biosynthesis and extracellular matrix remodeling. The protein is specifically involved in TGF-beta signaling pathways which regulate cellular differentiation and tissue homeostasis. It also affects the BMP signaling pathways influencing developmental processes and tissue repair. During these pathways Kyphoscoliosis peptidase interacts with other molecules like collagen type I and type VI integrating into broader biological contexts.

Kyphoscoliosis peptidase perturbations associate primarily with Ehlers-Danlos syndrome a connective tissue disorder resulting in joint hypermobility and skin elasticity. Alterations in its function also relate to kyphoscoliotic EDS a subtype characterized by severe skeletal abnormalities. In these conditions its interaction with proteins like type VI collagen becomes disrupted further affecting connective tissue structure and highlighting its significance in maintaining tissue integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable cytoskeleton-associated protease required for normal muscle growth. Involved in function, maturation and stabilization of the neuromuscular junction. May act by cleaving muscle-specific proteins such as FLNC (By similarity).
See full target information KY
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advances in clinical and experimental medicine : official organ Wroclaw Medical University 30:691-699 PubMed34118147

2021

Upregulation of centromere protein K is crucial for lung adenocarcinoma cell viability and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Yongbo Wang,Yue Wang,Chunna Ren,Haicun Wang,Yang Zhang,Yunxia Xiu
View all publications
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