Rabbit Polyclonal L Glutamate antibody. Suitable for IHC-Fr, IHC-FoFr and reacts with Chemical samples. Cited in 12 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to L Glutamate.
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- Frontiers in neuroscience 15:8050702021Effects of Nrf1 in Hypothalamic Paraventricular Nucleus on Regulating the Blood Pressure During Hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Jing Yu,Tong Xiao,Xiao-Jing Liu,Ying Li,Jie Qi,Nianping Zhang,Li-Yan Fu,Kai-Li Liu,Yanjun Li,Yu-Ming KangPubMed 34938159
- International journal of molecular sciences 22:2021Immunoelectron Microscopic Characterization of Vasopressin-Producing Neurons in the Hypothalamo-Pituitary Axis of Non-Human Primates by Use of Formaldehyde-Fixed Tissues Stored at -25 °C for Several Years.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkito Otubo,Sho Maejima,Takumi Oti,Keita Satoh,Yasumasa Ueda,John F Morris,Tatsuya Sakamoto,Hirotaka SakamotoPubMed 34502087
- Disease models & mechanisms 13:2020Loss of the branched-chain α-ketoacid dehydrogenase complex results in neuronal dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui-Ying Tsai et. al.PubMed 32680850
- Molecular vision 26:277-2902020Proinflammatory cytokines trigger biochemical and neurochemical changes in mouse retinal explants exposed to hyperglycemic conditions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGaganashree Shivashankar et. al.PubMed 32300272
- Frontiers in neuroscience 14:1612020Evidence of Synaptic and Neurochemical Remodeling in the Retina of Aging Degus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLily Y-L Chang et. al.PubMed 32256305
- Nature communications 5:41592014Tonic inhibition in dentate gyrus impairs long-term potentiation and memory in an Alzheimer's [corrected] disease model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZheng Wu et. al.PubMed 24923909
- Zoology (Jena, Germany) 116:1-82012The elusive role of L-glutamate as an echinoderm neurotransmitter: evidence for its involvement in the control of crinoid arm muscles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIain C Wilkie et. al.PubMed 23141629
- The Journal of physiology 590:855-732012Optogenetic activation of LiGluR-expressing astrocytes evokes anion channel-mediated glutamate release.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongdong Li,Karine Hérault,Ehud Y Isacoff,Martin Oheim,Nicole RopertPubMed 22219341
- Disease models & mechanisms 5:248-582011Mutation of zebrafish dihydrolipoamide branched-chain transacylase E2 results in motor dysfunction and models maple syrup urine disease.Applications:ICC/IFReactive species:ZebrafishTimo Friedrich,Aaron M Lambert,Mark A Masino,Gerald B DownesPubMed 22046030
- The Journal of experimental biology 213:2104-152010Physiological and immunocytochemical evidence that glutamatergic neurotransmission is involved in the activation of arm autotomy in the featherstar Antedon mediterranea (Echinodermata: Crinoidea).Applications:IHC-RReactive species:StarfishI C Wilkie,A Barbaglio,W M Maclaren,M D Candia CarnevaliPubMed 20511525
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Thimerosal (merthiolate)
Constituents: Phosphate Buffer, 1% Goat Serum- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Chemical / Small Molecule corresponding to L Glutamate.
Reactivity data
Select an application| IHC-Fr | IHC-FoFr | |
|---|---|---|
| Chemical | Tested | Expected |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes - |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info - | Notes Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur. |
Target data
Alternative names
L glutamate
Recommended products
Rabbit Polyclonal L Glutamate antibody. Suitable for IHC-Fr, IHC-FoFr and reacts with Chemical samples. Cited in 12 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to L Glutamate.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Thimerosal (merthiolate)
Constituents: Phosphate Buffer, 1% Goat Serum- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Chemical / Small Molecule corresponding to L Glutamate.
- Purity
- IgG fraction
- Specificity
The antibody is calibrated against a spectrum of antigens to assure hapten selectivity. No measurable cross-reactivity (<1:1000) was detected against glutamate in peptides or proteins. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, D/L-aspartate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:20) was detected against D-glutamate. Significant cross-reactivity (1:8) was detected against free NAAG in competition assays (NAAG is not retained in aldehyde-fixed tissue).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
The product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
L-glutamate also called glutamic acid is an important neurotransmitter in the central nervous system. This small amino acid has a molecular weight of 147.13 g/mol. Neurons and glial cells both express L-glutamate which allows it to function widely in the brain. Synaptic terminals release L-glutamate into the synaptic cleft where it binds to glutamate receptors on the postsynaptic neuron to propagate neural signals.
- Biological function summary
L-glutamate serves key roles in synaptic plasticity and cognitive functions including learning and memory. It acts as the main excitatory neurotransmitter in the human brain and is essential for normal brain activities. L-glutamate does not belong to a protein complex but it operates closely with other neurotransmitters and their receptors ensuring proper signal transmission between neurons.
- Pathways
L-glutamate is involved in synaptic signaling and metabolic pathways like the glutamatergic synapse pathway. This amino acid interacts with proteins such as NMDA and AMPA receptors that are integral to these pathways. The proper functioning of these pathways is critical for maintaining excitatory neurotransmission and overall brain health.
- Associated diseases and disorders
Irregular L-glutamate signaling relates to conditions such as Alzheimer’s disease and epilepsy. In Alzheimer’s disease altered glutamate signaling contributes to neurodegeneration with NMDA receptor overactivation playing a role. In epilepsy excessive glutamate release leads to hyperexcitation contributing to seizures. Understanding these associations helps to identify potential therapeutic targets for treating these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
This image is courtesy of a customer review submitted by Patricia BogdanovImmunohistochemistry (Frozen sections) - Anti-L Glutamate antibody (ab9440)
ab9440 staining L Gluramate in Mouse retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol, permeabilized with 0.025% TBS-Triton X-100 and blocked with 5% serum for 1 hour. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 18 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
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