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AB225916

Anti-L-Kynurenine Hydrolase antibody

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(2 Publications)

Rabbit Polyclonal L-Kynurenine Hydrolase antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Kynureninase aa 1-350.

View Alternative Names

Kynureninase, L-kynurenine hydrolase, KYNU

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L-Kynurenine Hydrolase antibody (AB225916)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L-Kynurenine Hydrolase antibody (AB225916)

Paraffin-embedded human placenta tissue stained for L-Kynurenine Hydrolase using ab225916 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L-Kynurenine Hydrolase antibody (AB225916)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L-Kynurenine Hydrolase antibody (AB225916)

Paraffin-embedded human liver tissue stained for L-Kynurenine Hydrolase using ab225916 at 1/100 dilution in immunohistochemical analysis.

Immunoprecipitation - Anti-L-Kynurenine Hydrolase antibody (AB225916)
  • IP

Supplier Data

Immunoprecipitation - Anti-L-Kynurenine Hydrolase antibody (AB225916)

L-Kynurenine Hydrolase was immunoprecipitated from 500 μg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 μg ab225916. For Western Blot analysis, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.

Lane 1 : Rabbit monoclonal IgG IP in HeLa whole cell lysate.

Lane 2 : ab225916 IP in HeLa whole cell lysate.

Lane 3 : HeLa whole cell lysate 20 μg (Input).

All lanes:

Immunoprecipitation - Anti-L-Kynurenine Hydrolase antibody (ab225916)

Predicted band size: 52 kDa

false

Western blot - Anti-L-Kynurenine Hydrolase antibody (AB225916)
  • WB

Supplier Data

Western blot - Anti-L-Kynurenine Hydrolase antibody (AB225916)

All lanes:

Western blot - Anti-L-Kynurenine Hydrolase antibody (ab225916) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 2:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Kynureninase aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

Q16719

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L-Kynurenine Hydrolase also known as L-Kynureninase is an enzyme with a mass of approximately 47 kDa. It acts as an important player in the kynurenine pathway by catalyzing the hydrolysis of L-kynurenine into anthranilic acid. This enzymatic activity is important for the degradation of tryptophan. L-Kynureninase is expressed in various tissues including the liver and brain reflecting its importance in systemic metabolic processes.
Biological function summary

L-Kynurenine Hydrolase participates in tryptophan catabolism affecting amino acid metabolism. It operates within the larger enzyme complex involved in the kynurenine pathway which is important for producing metabolites that have neurological and immunological functions. The activity of L-Kynureninase can influence the levels of pathway intermediates which are related to neurotransmitter synthesis and immune responses.

Pathways

This enzyme significantly contributes to the kynurenine pathway which is a major route for tryptophan metabolism. The pathway provides a link to the biosynthesis of nicotinamide adenine dinucleotide (NAD+) an essential coenzyme in cellular respiration. Proteins such as kynurenine 3-monooxygenase and aminocarboxymuconate semialdehyde decarboxylase are closely related to L-Kynureninase in these pathways interacting at different points in the metabolic sequence.

Altered L-Kynurenine Hydrolase activity is associated with conditions like neurodegenerative diseases and certain autoimmune disorders. For instance disruptions in its function can contribute to the development of neurological disorders such as Alzheimer's disease due to imbalanced neuroactive metabolite levels. It also has a connection with proteins like quinolinic acid a neurotoxin that may accumulate when L-Kynureninase activity is impaired linking it to potential damage in neural tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.
See full target information Kynureninase
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biochemical pharmacology 203:115187 PubMed35878796

2022

Human 5-lipoxygenase regulates transcription by association to euchromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Marius Kreiß,Julia H Oberlis,Sabine Seuter,Iris Bischoff-Kont,Duran Sürün,Dominique Thomas,Tamara Göbel,Tobias Schmid,Olof Rådmark,Ralf P Brandes,Robert Fürst,Ann-Kathrin Häfner,Dieter Steinhilber

Oncology reports 46: PubMed33982764

2021

Long non‑coding RNA NEAT1 regulates glioma cell proliferation and apoptosis by competitively binding to microRNA‑324‑5p and upregulating KCTD20 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jiale Zhang,Yangyang Li,Yuqi Liu,Guangzhi Xu,Yue Hei,Xiaoming Lu,Weiping Liu
View all publications
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