Rabbit Polyclonal L-Kynurenine Hydrolase antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KYNU aa 1-350.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KYNU aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q16719
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
Target data
Function
Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.
Alternative names
Kynureninase, L-kynurenine hydrolase, KYNU
Recommended products
Rabbit Polyclonal L-Kynurenine Hydrolase antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KYNU aa 1-350.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KYNU aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q16719
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
L-Kynurenine Hydrolase also known as L-Kynureninase is an enzyme with a mass of approximately 47 kDa. It acts as an important player in the kynurenine pathway by catalyzing the hydrolysis of L-kynurenine into anthranilic acid. This enzymatic activity is important for the degradation of tryptophan. L-Kynureninase is expressed in various tissues including the liver and brain reflecting its importance in systemic metabolic processes.
- Biological function summary
L-Kynurenine Hydrolase participates in tryptophan catabolism affecting amino acid metabolism. It operates within the larger enzyme complex involved in the kynurenine pathway which is important for producing metabolites that have neurological and immunological functions. The activity of L-Kynureninase can influence the levels of pathway intermediates which are related to neurotransmitter synthesis and immune responses.
- Pathways
This enzyme significantly contributes to the kynurenine pathway which is a major route for tryptophan metabolism. The pathway provides a link to the biosynthesis of nicotinamide adenine dinucleotide (NAD+) an essential coenzyme in cellular respiration. Proteins such as kynurenine 3-monooxygenase and aminocarboxymuconate semialdehyde decarboxylase are closely related to L-Kynureninase in these pathways interacting at different points in the metabolic sequence.
- Associated diseases and disorders
Altered L-Kynurenine Hydrolase activity is associated with conditions like neurodegenerative diseases and certain autoimmune disorders. For instance disruptions in its function can contribute to the development of neurological disorders such as Alzheimer's disease due to imbalanced neuroactive metabolite levels. It also has a connection with proteins like quinolinic acid a neurotoxin that may accumulate when L-Kynureninase activity is impaired linking it to potential damage in neural tissues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-L-Kynurenine Hydrolase antibody (ab225916)
All lanes: Western blot - Anti-L-Kynurenine Hydrolase antibody (ab225916) at 1/1000 dilution
Lane 1: A549 (human lung carcinoma cell line) whole cell lysate
Lane 2: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
SecondaryAll lanes: Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L-Kynurenine Hydrolase antibody (ab225916)
Paraffin-embedded human liver tissue stained for L-Kynurenine Hydrolase using ab225916 at 1/100 dilution in immunohistochemical analysis.
Immunoprecipitation - Anti-L-Kynurenine Hydrolase antibody (ab225916)
L-Kynurenine Hydrolase was immunoprecipitated from 500 μg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 μg ab225916. For Western Blot analysis, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
Lane 1: Rabbit monoclonal IgG IP in HeLa whole cell lysate.
Lane 2: ab225916 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 μg (Input).
All lanes: Immunoprecipitation - Anti-L-Kynurenine Hydrolase antibody (ab225916)
Predicted band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L-Kynurenine Hydrolase antibody (ab225916)
Paraffin-embedded human placenta tissue stained for L-Kynurenine Hydrolase using ab225916 at 1/100 dilution in immunohistochemical analysis.
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