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AB202978

Anti-L12 antibody

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Rabbit Polyclonal L12 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human RSL1D1 aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CATX11, CSIG, PBK1, L12, RSL1D1, Ribosomal L1 domain-containing protein 1, CATX-11, Cellular senescence-inhibited gene protein, Protein PBK1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L12 antibody (AB202978)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L12 antibody (AB202978)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling L12 with ab202978 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Western blot - Anti-L12 antibody (AB202978)
  • WB

Supplier Data

Western blot - Anti-L12 antibody (AB202978)

12% SDS-PAGE

All lanes:

Western blot - Anti-L12 antibody (ab202978) at 1/200 dilution

Lane 1:

Rat brain lysate

Lane 2:

Rat kidney lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

Predicted band size: 55 kDa

Observed band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RSL1D1 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O76021

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>(or 1/50 - 1/200 if using a fluorescent secondary antibody).</p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L12 also known as the 50S ribosomal protein L12 is an integral component of the bacterial ribosome. It plays a mechanical role in protein synthesis by facilitating the binding of translation factors. It's expressed in prokaryotic organisms and has a molecular mass of approximately 12 kDa. Its structural placement in the ribosome is essential for the proper functioning of the ribosomal machinery enabling efficient protein translation.
Biological function summary

L12 is an essential part of the ribosomal complex a multi-component structure responsible for protein synthesis. As part of the ribosomal stalk L12 functions in recruiting and interacting with GTPases which are important for translation accuracy and efficiency. Its role in the ribosome is critical for the interaction with elongation factors EF-Tu and EF-G ensuring the correct assembly and translocation of aminoacyl-tRNAs during protein synthesis.

Pathways

L12's involvement in protein translation places it at the heart of the central dogma of molecular biology. It participates in the translation pathway closely interacting with proteins like EF-Tu and EF-G. These interactions facilitate the transition of ribosomes through various stages of the translation cycle ensuring proper protein synthesis. The 50S ribosomal subunit which contains L12 cooperates with the 30S subunit in the intricate process of mRNA decoding and peptide bond formation.

L12's function connects to antibiotic resistance in bacteria due to its role in ribosomal activity. Modifications or mutations in ribosomal components including L12 may result in resistance to antibiotics that target the ribosome such as erythromycin. The involvement of L12 in diseases also ties it to ribosomal protein mutations which can lead to translational dysfunctions associated with pathological conditions. The study of L12 provides insights into understanding and combating bacterial infections as well as informing the development of new antibiotics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates cellular senescence through inhibition of PTEN translation. Acts as a pro-apoptotic regulator in response to DNA damage.
See full target information RSL1D1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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