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AB254850

Anti-L17 antibody

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Rabbit Polyclonal L17 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TPGS2 aa 1-100.

View Alternative Names

C18orf10, HMFN0601, L17, TPGS2, Tubulin polyglutamylase complex subunit 2, PGs2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L17 antibody (AB254850)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L17 antibody (AB254850)

Paraffin-embedded human testis tissue stained for L17 using ab254850 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-L17 antibody (AB254850)
  • WB

Supplier Data

Western blot - Anti-L17 antibody (AB254850)

All lanes:

Western blot - Anti-L17 antibody (ab254850) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T cell lysate

Lane 2:

L17 overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TPGS2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q68CL5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L17 also known as ribosomal protein L17 is an important component of the 60S subunit of the eukaryotic ribosome. It has a molecular mass of approximately 22 kDa. L17 is expressed in various tissues throughout the body with particularly high expression levels in rapidly-dividing cells. As a structural element of the large ribosomal subunit L17 plays a role in the core processes of protein synthesis.
Biological function summary

The function of protein L17 involves participation in the formation of the ribosome complex. It interacts with other ribosomal proteins and rRNA molecules to ensure the accurate assembly of the ribosome. This assembly allows the ribosomal machinery to facilitate efficient translation of mRNA into polypeptides which are subsequently folded into functional proteins essential for cellular functions and proliferation.

Pathways

The protein L17 engages with translation-related pathways notably the ribosomal biogenesis pathway and the mTOR signaling pathway. The mTOR pathway regulates cell growth and metabolism in response to nutrients stress and growth factors. As part of the ribosome L17 indirectly associates with proteins like RPL5 and RPL11 essential for ribosome biogenesis and function forming a link between ribosome assembly and cellular regulation.

Ribosomal protein L17 has implications in ribosomopathies such as Diamond-Blackfan Anemia which is characterized by impaired ribosome biogenesis and function. Mutations affecting L17 and its associated proteins such as RPL5 and RPL11 can disrupt normal ribosomal biogenesis leading to developmental abnormalities and increased susceptibility to specific cancers due to the imbalance in protein synthesis and cellular homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Subunit of the tubulin polyglutamylase complex (TPGC). The complex mediates cilia and flagella polyglutamylation which is essential for their biogenesis and motility.
See full target information TPGS2
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Product promise

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