Name: Anti-L1CAM antibody [EPR18750]
SKU: ab208155
Rating: 4 (3 reviews)

Rabbit Recombinant Monoclonal L1CAM antibody. Suitable for IP, WB, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 8 publications.

Images

Western blot - Anti-L1CAM antibody [EPR18750] (AB208155), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P
Human	Tested	Tested	Tested
Mouse	Expected	Tested	Tested
Rat	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/40	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/40	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Target data

See full target information L1CAM

Function

Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.

Alternative names

CD171, CAML1, MIC5, L1CAM, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1

Recommended products

Rabbit Recombinant Monoclonal L1CAM antibody. Suitable for IP, WB, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 8 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR18750
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

L1CAM also known as neural cell adhesion molecule L1 is a transmembrane protein with a molecular weight of approximately 200-220 kDa. The L1CAM protein belongs to the immunoglobulin superfamily and is heavily glycosylated. It plays a significant role in cell adhesion processes within the nervous system. L1CAM is expressed in a variety of tissues including the brain kidney and peripheral nerves and is notably present in neuronal cells where it engages in cell-cell interactions. Researchers frequently use L1CAM IHC ELISA methods and specific fluorescent markers like Alexa Fluor 568 to study its expression patterns and localization.

Biological function summary

L1CAM facilitates neuronal migration axonal growth and synaptic plasticity. This protein participates in the formation and maintenance of neural networks. It interacts with other cell adhesion molecules and components of the extracellular matrix. L1CAM acts as an important modulator within neuronal signaling complexes which impacts the development and regeneration of the nervous system. Insights into its structural and cell interaction properties have made it a focus for understanding nervous system functions.

Pathways

L1CAM participates extensively in the MAPK and PI3K/AKT pathways. These pathways contribute to cellular growth survival and programmed cell death. The protein interacts with other molecules such as integrins and FGFRs to propagate signaling cascades important for cellular response mechanisms. These connections help modulate cytoskeletal dynamics influencing cellular adhesion and motility needed during brain development and response to injury.

Associated diseases and disorders

L1CAM has been associated with hydrocephalus and some cancers. Mutations in the L1CAM gene can lead to L1 syndrome which includes a spectrum of neurological disorders particularly hydrocephalus. In cancer overexpression or altered regulation of L1CAM may correlate with tumor aggressiveness and metastatic potential. L1CAM's association with integrin and cadherin proteins underlines its relevance in pathological states and highlights a potential target for therapeutic interventions.

Product promise

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Full details and terms and conditions can be found here:
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16 product images

Western blot - Anti-L1CAM antibody [EPR18750] (ab208155)
Lanes 1-2: Merged signal (red and green). Green - ab208155 observed at 220 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007 observed at 125 kDa.
ab208155 was shown to react with L1CAM in wild-type HeLa cells in Western blot. Loss of signal was observed when knockout sample Human L1CAM knockout HeLa cell lysate ab263786 was used. Wild-type and L1CAM knockout samples were subjected to SDS-PAGE. ab208155 and Anti-Vinculin antibody [VIN-54] (Anti-Vinculin antibody [VIN-54] ab130007) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-L1CAM antibody [EPR18750] (ab208155) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: L1CAM knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human L1CAM knockout HeLa cell line (Human L1CAM knockout HeLa cell line ab255401)
Secondary
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 140 kDa
Observed band size: 124 kDa, 220 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] (ab208155)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling L1CAM with ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on the mouse cerebrum is observed.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-L1CAM antibody [EPR18750] (ab208155)
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 30seconds; Lane 3: 3 minutes.
The product binds to the full length L1CAM and the 140KD fragment. Plasmin cleaves L1CAM at the FN3 repeat to produce 140 kDa and 85 kDa fragments (PMID: 7542658;PMID: 20840789). The 140 kDa fragment is where the immunogen is located.
All lanes: Western blot - Anti-L1CAM antibody [EPR18750] (ab208155) at 1/2000 dilution
Lane 1: Human fetal brain lysate at 20 µg
Lane 2: Human cerebellum lysate at 20 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 140 kDa
Observed band size: 140 kDa, 200-220 kDa
Western blot - Anti-L1CAM antibody [EPR18750] (ab208155)
Blocking/Dilution buffer: 5% NFDM/TBST.
The product binds to the full length L1CAM and the 140KD fragment. Plasmin cleaves L1CAM at the FN3 repeat to produce 140 kDa and 85 kDa fragments (PMID: 7542658; PMID: 20840789). The 140 kDa fragment is where the immunogen is located.
All lanes: Western blot - Anti-L1CAM antibody [EPR18750] (ab208155) at 1/2000 dilution
Lane 1: Rat brain lysate at 20 µg
Lane 2: Rat cerebellum lysate at 20 µg
Lane 3: Rat hippocampus lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 140 kDa
Observed band size: 140 kDa, 200-220 kDa
Exposure time: 3min
Western blot - Anti-L1CAM antibody [EPR18750] (ab208155)
Blocking/Dilution buffer: 5% NFDM/TBST.
The product binds to the full length L1CAM and the 140KD fragment. Plasmin cleaves L1CAM at the FN3 repeat to produce 140 kDa and 85 kDa fragments (PMID: 7542658;PMID: 20840789). The 140 kDa fragment is where the immunogen is located.
All lanes: Western blot - Anti-L1CAM antibody [EPR18750] (ab208155) at 1/1000 dilution
Lane 1: Mouse cerebellum lysate at 10 µg
Lane 2: Mouse brain lysate at 10 µg
Lane 3: A-375 (Human malignant melanoma cell line) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 140 kDa
Observed band size: 140 kDa, 200-220 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] (ab208155)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling L1CAM with ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Membrane staining on a part of Human kidney tubules is observed.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] (ab208155)
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling L1CAM with ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Membrane staining on the tumor cells of Human stomach cancer is observed.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] (ab208155)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling L1CAM with ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative staining on the Human liver.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] (ab208155)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling L1CAM with ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Mainly membrane staining on the nerve tract of mouse colon is observed.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-L1CAM antibody [EPR18750] (ab208155)
False colour image of Western blot: Anti-L1CAM antibody [EPR18750] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab208155 was shown to bind specifically to L1CAM. A band was observed at 220 kDa in wild-type HeLa cell lysates with no signal observed at this size in L1CAM knockout cell line Human L1CAM knockout HeLa cell line ab273836 (knockout cell lysate Human L1CAM knockout HeLa cell lysate ab273790). The band observed in the knockout lysate lane below 220 kDa is likely to represent a truncated form of L1CAM. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and L1CAM knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-L1CAM antibody [EPR18750] (ab208155) at 1/1000 dilution
Lanes 1 and 3: Wild-type HeLa cell lysate
Lanes 2 and 4: L1CAM knockout HeLa cell lysate
Lane 5: A375 cell lysate
Lane 6: MOLT-4 cell lysate
Performed under reducing conditions.
Predicted band size: 140 kDa
Observed band size: 220 kDa
Immunoprecipitation - Anti-L1CAM antibody [EPR18750] (ab208155)
L1CAM was immunoprecipitated from 1mg of Human cerebellum lysate with ab208155 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab208155 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human cerebellum lysate 10μg (Input).
Lane 2: ab208155 IP in Human cerebellum lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab208155 in Human cerebellum lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-L1CAM antibody [EPR18750] (ab208155)
Predicted band size: 140 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] (ab208155)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling L1CAM with ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative staining on the mouse testis.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-L1CAM antibody [EPR18750] (ab208155)
L1CAM was immunoprecipitated from 1mg of Rat brain whole cell lysate with ab208155 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab208155 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Rat brain whole cell lysate 10μg (Input).
Lane 2: ab208155 IP in Rat brain whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab208155 in Rat brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-L1CAM antibody [EPR18750] (ab208155)
Predicted band size: 140 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] (ab208155)
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling L1CAM with ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on the molecular layer of the rat cerebellar cortex is observed.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] (ab208155)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling L1CAM with ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on the nerve tract of the rat colon is observed.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] (ab208155)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling L1CAM with ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative staining on the rat skeletal muscle.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.