Name: Anti-L1CAM antibody [EPR18750] - BSA and Azide free
SKU: ab271982
Rating: 1 (1 reviews)

Rabbit Recombinant Monoclonal L1CAM antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Rat, Human, Mouse samples.

Images

Western blot - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (AB271982), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P
Human	Tested	Tested	Tested
Mouse	Expected	Tested	Tested
Rat	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes -
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Associated Products

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Target data

See full target information L1CAM

Function

Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.

Alternative names

CD171, CAML1, MIC5, L1CAM, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1

Recommended products

Rabbit Recombinant Monoclonal L1CAM antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Rat, Human, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR18750
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab271982 is the carrier-free version of Anti-L1CAM antibody [EPR18750] ab208155.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

L1CAM also known as neural cell adhesion molecule L1 is a transmembrane protein with a molecular weight of approximately 200-220 kDa. The L1CAM protein belongs to the immunoglobulin superfamily and is heavily glycosylated. It plays a significant role in cell adhesion processes within the nervous system. L1CAM is expressed in a variety of tissues including the brain kidney and peripheral nerves and is notably present in neuronal cells where it engages in cell-cell interactions. Researchers frequently use L1CAM IHC ELISA methods and specific fluorescent markers like Alexa Fluor 568 to study its expression patterns and localization.

Biological function summary

L1CAM facilitates neuronal migration axonal growth and synaptic plasticity. This protein participates in the formation and maintenance of neural networks. It interacts with other cell adhesion molecules and components of the extracellular matrix. L1CAM acts as an important modulator within neuronal signaling complexes which impacts the development and regeneration of the nervous system. Insights into its structural and cell interaction properties have made it a focus for understanding nervous system functions.

Pathways

L1CAM participates extensively in the MAPK and PI3K/AKT pathways. These pathways contribute to cellular growth survival and programmed cell death. The protein interacts with other molecules such as integrins and FGFRs to propagate signaling cascades important for cellular response mechanisms. These connections help modulate cytoskeletal dynamics influencing cellular adhesion and motility needed during brain development and response to injury.

Associated diseases and disorders

L1CAM has been associated with hydrocephalus and some cancers. Mutations in the L1CAM gene can lead to L1 syndrome which includes a spectrum of neurological disorders particularly hydrocephalus. In cancer overexpression or altered regulation of L1CAM may correlate with tumor aggressiveness and metastatic potential. L1CAM's association with integrin and cadherin proteins underlines its relevance in pathological states and highlights a potential target for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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15 product images

Western blot - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
This data was developed using Anti-L1CAM antibody [EPR18750] ab208155, the same antibody clone in a different buffer formulation:
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 and 2: 30seconds; Lane 3: 3 minutes.
The product binds to the full length L1CAM and the 140KD fragment. Plasmin cleaves L1CAM at the FN3 repeat to produce 140 kDa and 85 kDa fragments (PMID: 7542658;PMID: 20840789). The 140 kDa fragment is where the immunogen is located.
All lanes: Western blot - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982) at 1/2000 dilution
Lane 1: Human fetal brain lysate at 20 µg
Lane 2: Human cerebellum lysate at 20 µg
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 140 kDa
Observed band size: 140 kDa, 200-220 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling L1CAM with Anti-L1CAM antibody [EPR18750] ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on the mouse cerebrum is observed.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-L1CAM antibody [EPR18750] ab208155).
Western blot - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
This data was developed using Anti-L1CAM antibody [EPR18750] ab208155, the same antibody clone in a different buffer formulation:
Blocking/Dilution buffer: 5% NFDM/TBST.
The product binds to the full length L1CAM and the 140KD fragment. Plasmin cleaves L1CAM at the FN3 repeat to produce 140 kDa and 85 kDa fragments (PMID: 7542658; PMID: 20840789). The 140 kDa fragment is where the immunogen is located.
All lanes: Western blot - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982) at 1/2000 dilution
Lane 1: Rat brain lysate at 20 µg
Lane 2: Rat cerebellum lysate at 20 µg
Lane 3: Rat hippocampus lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 140 kDa
Observed band size: 140 kDa, 200-220 kDa
Exposure time: 3min
Western blot - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
This data was developed using Anti-L1CAM antibody [EPR18750] ab208155, the same antibody clone in a different buffer formulation:
Blocking/Dilution buffer: 5% NFDM/TBST.
The product binds to the full length L1CAM and the 140KD fragment. Plasmin cleaves L1CAM at the FN3 repeat to produce 140 kDa and 85 kDa fragments (PMID: 7542658;PMID: 20840789). The 140 kDa fragment is where the immunogen is located.
All lanes: Western blot - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982) at 1/1000 dilution
Lane 1: Mouse cerebellum lysate at 10 µg
Lane 2: Mouse brain lysate at 10 µg
Lane 3: A-375 (Human malignant melanoma cell line) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 140 kDa
Observed band size: 140 kDa, 200-220 kDa
Exposure time: 3min
Western blot - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
This data was developed using Anti-L1CAM antibody [EPR18750] ab208155, the same antibody clone in a different buffer formulation:
Lanes 1 - 2: Merged signal (red and green). Green - Anti-L1CAM antibody [EPR18750] ab208155 observed at 220 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007 observed at 125 kDa.
Anti-L1CAM antibody [EPR18750] ab208155 was shown to react with L1CAM in wild-type HeLa. Loss of signal was observed when knockout cell line Human L1CAM knockout HeLa cell line ab255401 (knockout cell lysate Human L1CAM knockout HeLa cell lysate ab263786) was used. Wild-type and L1CAM knockout samples were subjected to SDS-PAGE. Anti-L1CAM antibody [EPR18750] ab208155 and Anti-Vinculin antibody [VIN-54] (Anti-Vinculin antibody [VIN-54] ab130007) were incubated overnight at 4^°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Lane 1: Western blot - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982) at 1/1000 dilution
Lane 2: Western blot - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: L1CAM knockout HeLa cell lysate at 20 µg
Predicted band size: 140 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling L1CAM with Anti-L1CAM antibody [EPR18750] ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Membrane staining on a part of Human kidney tubules is observed.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-L1CAM antibody [EPR18750] ab208155).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling L1CAM with Anti-L1CAM antibody [EPR18750] ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Membrane staining on the tumor cells of Human stomach cancer is observed.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-L1CAM antibody [EPR18750] ab208155).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling L1CAM with Anti-L1CAM antibody [EPR18750] ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative staining on the Human liver.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-L1CAM antibody [EPR18750] ab208155).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling L1CAM with Anti-L1CAM antibody [EPR18750] ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Mainly membrane staining on the nerve tract of mouse colon is observed.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-L1CAM antibody [EPR18750] ab208155).
Immunoprecipitation - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
L1CAM was immunoprecipitated from 1mg of Human cerebellum lysate with Anti-L1CAM antibody [EPR18750] ab208155 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-L1CAM antibody [EPR18750] ab208155 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

Lane 1: Human cerebellum lysate 10μg (Input).
Lane 2: Anti-L1CAM antibody [EPR18750] ab208155 IP in Human cerebellum lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-L1CAM antibody [EPR18750] ab208155 in Human cerebellum lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-L1CAM antibody [EPR18750] ab208155).
All lanes: Immunoprecipitation - Anti-L1CAM antibody [EPR18750] (Anti-L1CAM antibody [EPR18750] ab208155)
Predicted band size: 140 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling L1CAM with Anti-L1CAM antibody [EPR18750] ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative staining on the mouse testis.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-L1CAM antibody [EPR18750] ab208155).
Immunoprecipitation - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
L1CAM was immunoprecipitated from 1mg of Rat brain whole cell lysate with Anti-L1CAM antibody [EPR18750] ab208155 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-L1CAM antibody [EPR18750] ab208155 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

Lane 1: Rat brain whole cell lysate 10μg (Input).
Lane 2: Anti-L1CAM antibody [EPR18750] ab208155 IP in Rat brain whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-L1CAM antibody [EPR18750] ab208155 in Rat brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-L1CAM antibody [EPR18750] ab208155).
All lanes: Immunoprecipitation - Anti-L1CAM antibody [EPR18750] (Anti-L1CAM antibody [EPR18750] ab208155)
Predicted band size: 140 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling L1CAM with Anti-L1CAM antibody [EPR18750] ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on the molecular layer of the rat cerebellar cortex is observed.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-L1CAM antibody [EPR18750] ab208155).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling L1CAM with Anti-L1CAM antibody [EPR18750] ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on the nerve tract of the rat colon is observed.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-L1CAM antibody [EPR18750] ab208155).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR18750] - BSA and Azide free (ab271982)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling L1CAM with Anti-L1CAM antibody [EPR18750] ab208155 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Negative staining on the rat skeletal muscle.
L1CAM specific staining most abundant on nervous system, distal kidney tubules, and tumor cells. [PMID: 16867862, PMID: 20044598].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-L1CAM antibody [EPR18750] ab208155).