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AB20148

Anti-L1CAM antibody [UJ127.11]

4

(5 Reviews)

|

(9 Publications)

Mouse Monoclonal L1CAM antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Tissue preparation containing L1CAM protein.

View Alternative Names

CD171, CAML1, MIC5, L1CAM, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1

1 Images
Western blot - Anti-L1CAM antibody [UJ127.11] (AB20148)
  • WB

Unknown

Western blot - Anti-L1CAM antibody [UJ127.11] (AB20148)

All lanes:

Western blot - Anti-L1CAM antibody [UJ127.11] (ab20148) at 5 µg/mL

All lanes:

SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP at 1/3000 dilution

Predicted band size: 140 kDa

Observed band size: 200-220 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UJ127.11

Isotype

IgG1

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Tissue preparation containing L1CAM protein. The exact immunogen used to generate this antibody is proprietary information.

P32004

Specificity

UJ127.11 may be useful in the diagnosis of embryonic tumours (e.g. neuroblastoma).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>It may also detect smaller cleavage fragments (please see Notes below).</p>" } } }
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Product details

L1CAM can be detected between 200-220 kD. In brain samples it is typically seen at ~ 200 kD. When the protein is overexpressed in vitro it is often detected as a doublet with bands at 200 and 220 kD. The unglycosylated, unprocessed L1CAM is ~ 140-150 kDa. The protein has 21 putative N-glycosylation sites on the extracellular portion of the protein which, when they are all glycosylated, results in a detected MW of 200-220 kD depending upon how many residues are actually glycosylated. L1CAM can be cleaved by the metalloprotease ADAM10 resulting in fragments of 180 kD and 40 kD. L1CAM can also be cleaved by plasmin resulting in fragments of 140 kD and 80 kD. In theory, therefore, one could detect bands at ~220, 200, 180, 140, 80 and 40 kD.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L1CAM also known as neural cell adhesion molecule L1 is a transmembrane protein with a molecular weight of approximately 200-220 kDa. The L1CAM protein belongs to the immunoglobulin superfamily and is heavily glycosylated. It plays a significant role in cell adhesion processes within the nervous system. L1CAM is expressed in a variety of tissues including the brain kidney and peripheral nerves and is notably present in neuronal cells where it engages in cell-cell interactions. Researchers frequently use L1CAM IHC ELISA methods and specific fluorescent markers like Alexa Fluor 568 to study its expression patterns and localization.
Biological function summary

L1CAM facilitates neuronal migration axonal growth and synaptic plasticity. This protein participates in the formation and maintenance of neural networks. It interacts with other cell adhesion molecules and components of the extracellular matrix. L1CAM acts as an important modulator within neuronal signaling complexes which impacts the development and regeneration of the nervous system. Insights into its structural and cell interaction properties have made it a focus for understanding nervous system functions.

Pathways

L1CAM participates extensively in the MAPK and PI3K/AKT pathways. These pathways contribute to cellular growth survival and programmed cell death. The protein interacts with other molecules such as integrins and FGFRs to propagate signaling cascades important for cellular response mechanisms. These connections help modulate cytoskeletal dynamics influencing cellular adhesion and motility needed during brain development and response to injury.

L1CAM has been associated with hydrocephalus and some cancers. Mutations in the L1CAM gene can lead to L1 syndrome which includes a spectrum of neurological disorders particularly hydrocephalus. In cancer overexpression or altered regulation of L1CAM may correlate with tumor aggressiveness and metastatic potential. L1CAM's association with integrin and cadherin proteins underlines its relevance in pathological states and highlights a potential target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
See full target information L1CAM
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

The journal of international advanced otology 21:1-8 PubMed39936830

2025

Analysis of L1 Cell Adhesion Molecule and Fucosyltransferase 8 Expression in Cells After Stretch and Human EACSCC Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Naotaro Akiyama,Tomomi Yamamoto-Fukuda,Hiromi Kojima

The American journal of surgical pathology 48:163-173 PubMed37994665

2023

Hybrid Oncocytic Tumors (HOTs) in Birt-Hogg-Dubé Syndrome Patients-A Tale of Two Cities: Sequencing Analysis Reveals Dual Lineage Markers Capturing the 2 Cellular Populations of HOT.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Ming Wang,Rahul Mannan,Yuping Zhang,Anya Chinnaiyan,Roshni Rangaswamy,Seema Chugh,Fengyun Su,Xuhong Cao,Rui Wang,Stephanie L Skala,Khaled S Hafez,Ulka Vaishampayan,Jesse Mckenney,Maria M Picken,Sounak Gupta,Reza Alaghehbandan,Maria Tretiakova,Pedram Argani,Arul M Chinnaiyan,Saravana M Dhanasekaran,Rohit Mehra

Neurobiology of disease 116:53-59 PubMed29705185

2018

Plasma α-synuclein and cognitive impairment in the Parkinson's Associated Risk Syndrome: A pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Wang,Anzari Atik,Tessandra Stewart,Carmen Ginghina,Patrick Aro,Kathleen F Kerr,John Seibyl,Danna Jennings,Poul Henning Jensen,Kenneth Marek,Min Shi,Jing Zhang

International journal of oncology 52:787-803 PubMed29328367

2018

Tumorigenic proteins upregulated in the MYCN-amplified IMR-32 human neuroblastoma cells promote proliferation and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Hayat Zaatiti,Jad Abdallah,Zeina Nasr,George Khazen,Anthony Sandler,Tamara J Abou-Antoun

Molecular cell 68:731-744.e9 PubMed29149598

2017

Harnessing BET Inhibitor Sensitivity Reveals AMIGO2 as a Melanoma Survival Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Fontanals-Cirera,Dan Hasson,Chiara Vardabasso,Raffaella Di Micco,Praveen Agrawal,Asif Chowdhury,Madeleine Gantz,Ana de Pablos-Aragoneses,Ari Morgenstern,Pamela Wu,Dan Filipescu,David Valle-Garcia,Farbod Darvishian,Jae-Seok Roe,Michael A Davies,Christopher R Vakoc,Eva Hernando,Emily Bernstein

Cancer cell 31:804-819.e7 PubMed28609658

2017

A Systems Biology Approach Identifies FUT8 as a Driver of Melanoma Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Praveen Agrawal,Barbara Fontanals-Cirera,Elena Sokolova,Samson Jacob,Christopher A Vaiana,Diana Argibay,Veronica Davalos,Meagan McDermott,Shruti Nayak,Farbod Darvishian,Mireia Castillo,Beatrix Ueberheide,Iman Osman,David Fenyö,Lara K Mahal,Eva Hernando

Annals of surgical oncology 14:1703-11 PubMed17211730

2007

L1 expression as a marker for poor prognosis, tumor progression, and short survival in patients with colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yoon-Jung Boo,Joong-Min Park,Jin Kim,Yang-Seok Chae,Byung-Wook Min,Jun-Won Um,Hong-Young Moon

Biochemical Society transactions 18:274 PubMed2379713

1990

Monoclonal antibody UJ127.11 recognizes the human homologue of mouse L1 cell adhesion molecule.

Applications

Unspecified application

Species

Unspecified reactive species

K Patel,F Kiely,F Rathjen,J Kemshead

International journal of cancer 31:187-95 PubMed6826247

1983

Monoclonal antibody UJ 127:11 detects a 220,000-240,000 kdal. glycoprotein present on a sub-set of neuroectodermally derived cells.

Applications

Unspecified application

Species

Unspecified reactive species

J T Kemshead,J Fritschy,J A Garson,P Allan,H Coakham,S Brown,U Asser
View all publications
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