JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB174437

Anti-L2/HNK-1 Carbohydrate antibody [ZN-12]

5

(1 Review)

|

(1 Publication)

Mouse Monoclonal L2/HNK-1 Carbohydrate antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.
1 Images
Immunocytochemistry/ Immunofluorescence - Anti-L2/HNK-1 Carbohydrate antibody [ZN-12] (AB174437)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-L2/HNK-1 Carbohydrate antibody [ZN-12] (AB174437)

Immunocytochemistry with SH-SY5Y cells stained with ab174437 in red and DAPI in blue, as a counter stain. Fixation : Paraformaldehyde fixed (4%, 20 min) Permeabilization : Triton X-100 permeabilized (0.1%, 10 min) Blocking : 10% Goat Serum/PBS (1 hour) Primary : ab174437 5 μg/mL (10% Goat Serum/PBS, 2hr at room temperature or overnight at 4°C). Secondary : Alexa 594 goat anti-mouse IgG1 used at a 1/1000 dilution in 10% Goat Serum/PBS for 1hr at RT. Washing : 3x 1% Goat Serum/PBS, 10 minutes/wash DAPI : 2 μM in 1% Goat Serum/PBS, 10 minutes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

zn-12

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Zebrafish": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
style
left-column

Properties and storage information

Form
Liquid
Purity
undefined SDS-PAGE
Purification notes
ab174437 is produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. Near homogeneity as judged by SDS-PAGE.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99.9% HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L2/HNK-1 Carbohydrate also known as CD57 antigen is a glycan structure recognized for its role in cell-cell interaction processes. This carbohydrate moiety has a distinct structure that includes sulfated glucuronic acid followed by a linear poly-N-acetyllactosamine. It usually appears in the nervous system as well as certain subsets of immune cells especially on natural killer (NK) and T lymphocytes. The molecular mass of the L2/HNK-1 carbohydrate is complex due to its glycosylation making exact mass variable depending on the glycoprotein or glycolipid to which it is attached.
Biological function summary

L2/HNK-1 Carbohydrate participates in neural cell recognition and migration assisting in neurodevelopmental processes. It is not part of a single complex; instead it interacts with multiple proteins and cell surface molecules contributing to signal transduction involved in synaptic stabilization and regeneration. This carbohydrate also plays a role in modulating immune cell activities and is considered important in identifying specific subpopulations of T and NK cells.

Pathways

L2/HNK-1 Carbohydrate belongs to key processes such as the cell adhesion and signal transduction pathways. It shares interactions with proteins involved in the neural cell adhesion molecule (NCAM) pathway facilitating synaptic plasticity and neuronal connectivity. Its involvement in immune pathways connects it to lectin proteins influencing T cell activation and immune response modulation.

Abnormal expression or dysfunction of L2/HNK-1 Carbohydrate relates to conditions like multiple sclerosis and certain cancers. It is associated with neurodegenerative processes where changes in its expression can affect neuronal repair and immune functions. In cancer particularly in certain lymphomas altered levels of CD57 antigen can indicate tumor infiltration by NK cells and T lymphocytes reflecting its engagement with immune regulatory proteins such as CD8.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information L2/HNK-1 Carbohydrate
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:67 PubMed40266594

2025

Microglia-Derived IL-6 Promotes Müller Glia Reprogramming and Proliferation in Zebrafish Retina Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xu,Yi Li,Xiangyu Li,Xuan Tan,Lihua Liu,Lining Cao,Hui Xu
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB212403

Anti-CD57 antibody [HNK-1 or Leu-7] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD57 antibody [HNK-1 or Leu-7] - BSA and Azide free (AB212403)

  • 5
  • 1
Primary Antibodies

AB154193

Anti-Von Willebrand Factor antibody [EPSISR15]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

  • 0
  • 0
Primary Antibodies

AB101318

Anti-Zfp281/ZNF281 antibody

Western blot - Anti-Zfp281/ZNF281 antibody (AB101318)

  • 5
  • 2
Primary Antibodies

AB18207

Anti-beta III Tubulin antibody - Neuronal Marker

BOND RX™ Validated|Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-beta III Tubulin antibody - Neuronal Marker (AB18207)

  • 5
  • 67

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com