Mouse Monoclonal L2/HNK-1 Carbohydrate antibody. Suitable for ICC/IF and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-L2/HNK-1 Carbohydrate antibody [ZN-12] (AB174437), expandable thumbnail](https://content.abcam.com/products/images/l2-hnk-1-carbohydrate-antibody-zn-12-ab174437--immunocytochemistry-immunofluorescence-img11836.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-L2/HNK-1 Carbohydrate antibody [ZN-12] (AB174437)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99.9% HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Zebrafish | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Recommended products
Mouse Monoclonal L2/HNK-1 Carbohydrate antibody. Suitable for ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99.9% HEPES buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- zn-12
- Light chain type
- kappa
- Concentration
- Purification notes
ab174437 is produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. Near homogeneity as judged by SDS-PAGE.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product was previously marketed under the MitoSciences sub-brand.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
L2/HNK-1 Carbohydrate also known as CD57 antigen is a glycan structure recognized for its role in cell-cell interaction processes. This carbohydrate moiety has a distinct structure that includes sulfated glucuronic acid followed by a linear poly-N-acetyllactosamine. It usually appears in the nervous system as well as certain subsets of immune cells especially on natural killer (NK) and T lymphocytes. The molecular mass of the L2/HNK-1 carbohydrate is complex due to its glycosylation making exact mass variable depending on the glycoprotein or glycolipid to which it is attached.
- Biological function summary
L2/HNK-1 Carbohydrate participates in neural cell recognition and migration assisting in neurodevelopmental processes. It is not part of a single complex; instead it interacts with multiple proteins and cell surface molecules contributing to signal transduction involved in synaptic stabilization and regeneration. This carbohydrate also plays a role in modulating immune cell activities and is considered important in identifying specific subpopulations of T and NK cells.
- Pathways
L2/HNK-1 Carbohydrate belongs to key processes such as the cell adhesion and signal transduction pathways. It shares interactions with proteins involved in the neural cell adhesion molecule (NCAM) pathway facilitating synaptic plasticity and neuronal connectivity. Its involvement in immune pathways connects it to lectin proteins influencing T cell activation and immune response modulation.
- Associated diseases and disorders
Abnormal expression or dysfunction of L2/HNK-1 Carbohydrate relates to conditions like multiple sclerosis and certain cancers. It is associated with neurodegenerative processes where changes in its expression can affect neuronal repair and immune functions. In cancer particularly in certain lymphomas altered levels of CD57 antigen can indicate tumor infiltration by NK cells and T lymphocytes reflecting its engagement with immune regulatory proteins such as CD8.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-L2/HNK-1 Carbohydrate antibody [ZN-12] (ab174437), expandable thumbnail](https://content.abcam.com/products/images/l2-hnk-1-carbohydrate-antibody-zn-12-ab174437--immunocytochemistry-immunofluorescence-img11836.jpg "Immunocytochemistry/ Immunofluorescence - Anti-L2/HNK-1 Carbohydrate antibody [ZN-12] (ab174437)")
Immunocytochemistry/ Immunofluorescence - Anti-L2/HNK-1 Carbohydrate antibody [ZN-12] (ab174437)
Immunocytochemistry with SH-SY5Y cells stained with ab174437 in red and DAPI in blue, as a counter stain. Fixation: Paraformaldehyde fixed (4%, 20 min) Permeabilization: Triton X-100 permeabilized (0.1%, 10 min) Blocking: 10% Goat Serum/PBS (1 hour) Primary: ab174437 5 μg/mL (10% Goat Serum/PBS, 2hr at room temperature or overnight at 4°C). Secondary: Alexa 594 goat anti-mouse IgG1 used at a 1/1000 dilution in 10% Goat Serum/PBS for 1hr at RT. Washing: 3x 1% Goat Serum/PBS, 10 minutes/wash DAPI: 2 μM in 1% Goat Serum/PBS, 10 minutes.
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