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AB97304

Anti-L3MBTL1 antibody

5

(1 Review)

|

(1 Publication)

Rabbit Polyclonal L3MBTL1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human L3MBTL1 aa 350-750.

View Alternative Names

KIAA0681, L3MBT, L3MBTL, L3MBTL1, Lethal(3)malignant brain tumor-like protein 1, H-l(3)mbt, H-l(3)mbt protein, L(3)mbt-like, L(3)mbt protein homolog

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-L3MBTL1 antibody (AB97304)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-L3MBTL1 antibody (AB97304)

Images show immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using 1/200 ab97304 alone (1) or merged with DNA probe (2) .

Western blot - Anti-L3MBTL1 antibody (AB97304)
  • WB

Unknown

Western blot - Anti-L3MBTL1 antibody (AB97304)

7.5% SDS PAGE

All lanes:

Western blot - Anti-L3MBTL1 antibody (ab97304) at 1/1000 dilution

All lanes:

Hela S3 whole cell lysate at 30 µg

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human L3MBTL1 aa 350-750. The exact immunogen used to generate this antibody is proprietary information.

Q9Y468

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L3MBTL1 also known as the MBT domain-containing protein L3MBTL1 is a protein that plays a role in chromatin remodeling. This protein has a molecular mass of approximately 83 kDa. It is expressed in various tissues including hematopoietic progenitor cells and the brain. L3MBTL1 interacts with DNA and histones participating in the modification of chromatin structure important for gene expression regulation.
Biological function summary

L3MBTL1 functions as a transcriptional repressor involved in cell cycle regulation and differentiation. It acts by binding to methylated lysines on histone tails affecting chromatin compaction and accessibility. L3MBTL1 is part of the Polycomb group (PcG) protein complex which maintains genes in a repressed state ensuring proper cell fate determination.

Pathways

L3MBTL1 is an important component in chromatin modification and transcriptional repression pathways. It participates in pathways such as the Polycomb Repressive Complex 1 (PRC1)-mediated gene silencing pathway. L3MBTL1 interacts with other Polycomb proteins such as BMI1 and RING1 to ensure effective repression of target genes.

L3MBTL1 has a strong connection to certain types of cancer and genetic syndromes. Alterations in L3MBTL1 expression or function can contribute to hematological malignancies like acute myeloid leukemia. It is also linked to developmental disorders due to its role in epigenetic regulation during development. L3MBTL1 may interact with proteins like EZH2 a related PcG protein influencing the progression of these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes : acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/H1-4 at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.
See full target information L3MBTL1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:5493 PubMed36123338

2022

Extracellular fibrinogen-binding protein released by intracellular Staphylococcus aureus suppresses host immunity by targeting TRAF3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaokai Zhang,Tingrong Xiong,Lin Gao,Yu Wang,Luxuan Liu,Tian Tian,Yun Shi,Jinyong Zhang,Zhuo Zhao,Dongshui Lu,Ping Luo,Weijun Zhang,Ping Cheng,Haiming Jing,Qiang Gou,Hao Zeng,Dapeng Yan,Quanming Zou
View all publications
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