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Rabbit Recombinant Monoclonal L3MBTL1 antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (AB259919), expandable thumbnail
  • Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (AB259919), expandable thumbnail
  • Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] (AB259919), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-L3MBTL1 antibody [EPR23810-121] (AB259919), expandable thumbnail
  • Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] (AB259919), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Tested
Mouse
Not recommended
Tested
Tested
Not recommended
Not recommended
Rat
Expected
Expected
Tested
Not recommended
Expected

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Rat
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Target data

Function

Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/H1-4 at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.

Alternative names

KIAA0681, L3MBT, L3MBTL, L3MBTL1, Lethal(3)malignant brain tumor-like protein 1, H-l(3)mbt, H-l(3)mbt protein, L(3)mbt-like, L(3)mbt protein homolog

Recommended products

Rabbit Recombinant Monoclonal L3MBTL1 antibody. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23810-121
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

L3MBTL1 also known as the MBT domain-containing protein L3MBTL1 is a protein that plays a role in chromatin remodeling. This protein has a molecular mass of approximately 83 kDa. It is expressed in various tissues including hematopoietic progenitor cells and the brain. L3MBTL1 interacts with DNA and histones participating in the modification of chromatin structure important for gene expression regulation.

Biological function summary

L3MBTL1 functions as a transcriptional repressor involved in cell cycle regulation and differentiation. It acts by binding to methylated lysines on histone tails affecting chromatin compaction and accessibility. L3MBTL1 is part of the Polycomb group (PcG) protein complex which maintains genes in a repressed state ensuring proper cell fate determination.

Pathways

L3MBTL1 is an important component in chromatin modification and transcriptional repression pathways. It participates in pathways such as the Polycomb Repressive Complex 1 (PRC1)-mediated gene silencing pathway. L3MBTL1 interacts with other Polycomb proteins such as BMI1 and RING1 to ensure effective repression of target genes.

Associated diseases and disorders

L3MBTL1 has a strong connection to certain types of cancer and genetic syndromes. Alterations in L3MBTL1 expression or function can contribute to hematological malignancies like acute myeloid leukemia. It is also linked to developmental disorders due to its role in epigenetic regulation during development. L3MBTL1 may interact with proteins like EZH2 a related PcG protein influencing the progression of these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919), expandable thumbnail

    Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)

    Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: HL-60 (PMID: 10445843 )

    The two reactive bands represent different isoforms

    Exposure time: 37 seconds

    All lanes: Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919) at 1/1000 dilution

    Lane 1: IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 2: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: HL-60 (human Acute Promyelocytic Leukemia promyeloblast) whole cell lysate at 20 µg

    Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 6: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 7: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 83 kDa

    Observed band size: 100 kDa

  • Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919), expandable thumbnail

    Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds

    All lanes: Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 83 kDa

    Observed band size: 100 kDa

  • Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919), expandable thumbnail

    Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)

    L3MBTL1 was immunoprecipitated from 0.35 mg IMR-32 (human neuroblastoma neuroblast) whole cell lysate 10 ug with ab259919 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259919 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: IMR-32 (human neuroblastoma neuroblast) whole cell lysate 10 ug

    Lane 2: ab259919 IP in IMR-32 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259919 in IMR-32 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds

    All lanes: Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)

    Predicted band size: 83 kDa

    Observed band size: 100 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized IMR-32 cells labelling L3MBTL1 with ab259919 at 1/50 (10.4 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in IMR-32 cells is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919), expandable thumbnail

    Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)

    L3MBTL1 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug with ab259919 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259919 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug

    Lane 2: ab259919 IP in Neuro-2a whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259919 in Neuro-2a whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds

    All lanes: Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)

    Predicted band size: 83 kDa

    Observed band size: 100 kDa

  • Flow Cytometry (Intracellular) - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-L3MBTL1 antibody [EPR23810-121] (ab259919)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized IMR-32 (human neuroblastoma neuroblast) cells labelling L3MBTL1 with ab259919 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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