AB281834

Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal L3MBTL1 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.

View Alternative Names

KIAA0681, L3MBT, L3MBTL, L3MBTL1, Lethal(3)malignant brain tumor-like protein 1, H-l(3)mbt, H-l(3)mbt protein, L(3)mbt-like, L(3)mbt protein homolog

6 Images

Flow Cytometry (Intracellular) - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

This data was developed using ab259919, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized IMR-32 (human neuroblastoma neuroblast) cells labelling L3MBTL1 with ab259919 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti-rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

This data was developed using ab259919, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized IMR-32 cells labelling L3MBTL1 with ab259919 at 1/50 (10.4 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing nuclear staining in IMR-32 cells is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Lab

Western blot - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

This data was developed using 259919, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 10 seconds.

All lanes:

Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (<a href='/en-us/products/primary-antibodies/l3mbtl1-antibody-epr23810-121-ab259919'>ab259919</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 83 kDa

Observed band size: 100 kDa

false

Supplier Data

Western blot - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

This data was developed using 259919, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : HL-60 (PMID : 10445843 )

The two reactive bands represent different isoforms

Exposure time : 37 seconds.

All lanes:

Western blot - Anti-L3MBTL1 antibody [EPR23810-121] (<a href='/en-us/products/primary-antibodies/l3mbtl1-antibody-epr23810-121-ab259919'>ab259919</a>) at 1/1000 dilution

Lane 1:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HL-60 (human Acute Promyelocytic Leukemia promyeloblast) whole cell lysate at 20 µg

Lane 5:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 6:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 7:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 83 kDa

Observed band size: 100 kDa

false

Supplier Data

Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

This data was developed using ab259919, the same antibody clone in a different buffer formulation.

L3MBTL1 was immunoprecipitated from 0.35 mg IMR-32 (human neuroblastoma neuroblast) whole cell lysate 10 ug with ab259919 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259919 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : IMR-32 (human neuroblastoma neuroblast) whole cell lysate 10 ug

Lane 2 : ab259919 IP in IMR-32 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259919 in IMR-32 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5.5 seconds.

All lanes:

Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] (<a href='/en-us/products/primary-antibodies/l3mbtl1-antibody-epr23810-121-ab259919'>ab259919</a>)

Predicted band size: 83 kDa

Observed band size: 100 kDa

false

Supplier Data

Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

This data was developed using ab259919, the same antibody clone in a different buffer formulation.

L3MBTL1 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug with ab259919 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259919 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug

Lane 2 : ab259919 IP in Neuro-2a whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259919 in Neuro-2a whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5.5 seconds.

All lanes:

Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] (<a href='/en-us/products/primary-antibodies/l3mbtl1-antibody-epr23810-121-ab259919'>ab259919</a>)

Predicted band size: 83 kDa

Observed band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23810-121

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab281834 is the carrier-free version of ab259919.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L3MBTL1 also known as the MBT domain-containing protein L3MBTL1 is a protein that plays a role in chromatin remodeling. This protein has a molecular mass of approximately 83 kDa. It is expressed in various tissues including hematopoietic progenitor cells and the brain. L3MBTL1 interacts with DNA and histones participating in the modification of chromatin structure important for gene expression regulation.

Biological function summary

L3MBTL1 functions as a transcriptional repressor involved in cell cycle regulation and differentiation. It acts by binding to methylated lysines on histone tails affecting chromatin compaction and accessibility. L3MBTL1 is part of the Polycomb group (PcG) protein complex which maintains genes in a repressed state ensuring proper cell fate determination.

Pathways

L3MBTL1 is an important component in chromatin modification and transcriptional repression pathways. It participates in pathways such as the Polycomb Repressive Complex 1 (PRC1)-mediated gene silencing pathway. L3MBTL1 interacts with other Polycomb proteins such as BMI1 and RING1 to ensure effective repression of target genes.

L3MBTL1 has a strong connection to certain types of cancer and genetic syndromes. Alterations in L3MBTL1 expression or function can contribute to hematological malignancies like acute myeloid leukemia. It is also linked to developmental disorders due to its role in epigenetic regulation during development. L3MBTL1 may interact with proteins like EZH2 a related PcG protein influencing the progression of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes : acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/H1-4 at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.

See full target information L3MBTL1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

Immunocytochemistry/ Immunofluorescence - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

Western blot - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

Western blot - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

Immunoprecipitation - Anti-L3MBTL1 antibody [EPR23810-121] - BSA and Azide free (AB281834)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Assay Kits

Primary Antibodies

Primary Antibodies

Product promise