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AB237628

Anti-L3MBTL2 antibody

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(1 Publication)

Rabbit Polyclonal L3MBTL2 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Lethal(3)malignant brain tumor-like protein 2 aa 1-250.

View Alternative Names

Lethal(3)malignant brain tumor-like protein 2, H-l(3)mbt-like protein 2, L(3)mbt-like protein 2, L3MBTL2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-L3MBTL2 antibody (AB237628)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-L3MBTL2 antibody (AB237628)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for L3MBTL2 (green) using ab237628 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

Western blot - Anti-L3MBTL2 antibody (AB237628)
  • WB

Supplier Data

Western blot - Anti-L3MBTL2 antibody (AB237628)

All lanes:

Western blot - Anti-L3MBTL2 antibody (ab237628) at 1/200 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 2:

Rat heart tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 79 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Lethal(3)malignant brain tumor-like protein 2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q969R5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The L3MBTL2 protein also known as Lethal(3)malignant brain tumor-like protein 2 is a chromatin-binding protein involved in transcriptional repression. It has an approximate mass of 88 kDa. L3MBTL2 plays a role in the Polycomb group of proteins which are known for chromatin modification and gene expression regulation. The protein is broadly expressed in various tissues with higher expression noted in the thymus and testis.
Biological function summary

L3MBTL2 contributes to maintaining gene silencing through its interaction with various chromatin-modifying complexes. As part of the PcG repression complex it binds to modified histones particularly those marked by methylation to suppress transcription. This role is important for proper development and cellular differentiation as it ensures genes turn off or on at the right times during cell cycle and differentiation processes.

Pathways

L3MBTL2 plays key roles in pathways controlling gene expression and cell cycle regulation. Through the Polycomb repressive complex it connects to pathways that influence cellular proliferation and differentiation. L3MBTL2 often interacts with proteins such as RING1B and EZH2 in these pathways facilitating the propagation of chromatin-associated silencing marks that help coordinate complex gene expression programs.

L3MBTL2 is linked to certain cancers and developmental disorders due to its role in gene regulation. Misregulation of L3MBTL2 has been observed in hematopoietic malignancies impacting the normal proliferation of blood cells. It shares connections with proteins like BMI1 in the context of these disorders highlighting its relevance in maintaining the balance of gene expression required for cellular homeostasis and preventing oncogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'.
See full target information Lethal(3)malignant brain tumor-like protein 2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 24:19-23 PubMed33318667

2020

Microglial G-dependent dynamics regulate brain network hyperexcitability.

Applications

Unspecified application

Species

Unspecified reactive species

Mario Merlini,Victoria A Rafalski,Keran Ma,Keun-Young Kim,Eric A Bushong,Pamela E Rios Coronado,Zhaoqi Yan,Andrew S Mendiola,Elif G Sozmen,Jae Kyu Ryu,Matthias G Haberl,Matthew Madany,Daniel Naranjo Sampson,Mark A Petersen,Sophia Bardehle,Reshmi Tognatta,Terry Dean,Rosa Meza Acevedo,Belinda Cabriga,Reuben Thomas,Shaun R Coughlin,Mark H Ellisman,Jorge J Palop,Katerina Akassoglou
View all publications
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