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AB68117

Anti-L3MBTL3 antibody

4

(2 Reviews)

|

(2 Publications)

Mouse Polyclonal L3MBTL3 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human L3MBTL3.

View Alternative Names

KIAA1798, MBT1, L3MBTL3, Lethal(3)malignant brain tumor-like protein 3, H-l(3)mbt-like protein 3, L(3)mbt-like protein 3, L3mbt-like 3, MBT-1

1 Images
Western blot - Anti-L3MBTL3 antibody (AB68117)
  • WB

Unknown

Western blot - Anti-L3MBTL3 antibody (AB68117)

All lanes:

Western blot - Anti-L3MBTL3 antibody (ab68117) at 1/500 dilution

Lane 1:

L3MBTL3 transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution

Predicted band size: 88 kDa

Observed band size: 100 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human L3MBTL3.

Q96JM7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L3MBTL3 also known as Lethal(3) Malignant Brain Tumor-Like Protein 3 is a member of the polycomb group proteins which regulate gene expression by remodeling chromatin. It has a molecular weight of approximately 69 kDa. L3MBTL3 is widely expressed in various tissues with significant levels in the brain and hematopoietic cells. This protein plays a role in chromatin compaction through its ability to recognize and bind mono- and dimethylated lysine residues on histones. These activity patterns classify it as a histone reader influencing the accessibility of transcriptionally active regions.
Biological function summary

L3MBTL3 affects cellular processes by managing chromatin structure which in turn regulates gene expression. It participates in chromatin remodeling as part of large multi-protein complexes. By interacting with different components L3MBTL3 modulates the transcription of oncogenes and tumor suppressors. It contributes to important cellular processes including cell-cycle progression and differentiation by establishing particular transcriptional profiles. The protein's role in these processes highlights its importance in maintaining normal cell function.

Pathways

L3MBTL3 participates in pathways that involve chromatin modification and gene silencing. It is associated with the Polycomb Repressive Complex 2 (PRC2) pathway which plays a role in maintaining transcriptional repression. This pathway involves key proteins such as EZH2 and SUZ12 which work alongside L3MBTL3 to modify histone marks thereby silencing target genes. This positioning within pathways makes it an integral component of transcriptional regulation by ensuring genes are restricted as necessary for proper development and cellular activity.

L3MBTL3 has associations with cancer and hematological abnormalities. Its dysregulation has links to certain types of leukemia where abnormal expression can lead to uncontrolled cell proliferation. Another condition tied to L3MBTL3 is brain malignancies due to perturbations in its normal function within neuronal cells. Interactions with proteins like EZH2 further connect L3MBTL3 to cancer pathways emphasizing the relevance of its careful regulation in maintaining cellular health and preventing disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Is a negative regulator of Notch target genes expression, required for RBPJ-mediated transcriptional repression (PubMed : 29030483). It recruits KDM1A to Notch-responsive elements and promotes KDM1A-mediated H3K4me demethylation (PubMed : 29030483). Involved in the regulation of ubiquitin-dependent degradation of a set of methylated non-histone proteins, including SOX2, DNMT1 and E2F1. It acts as an adapter recruiting the CRL4-DCAF5 E3 ubiquitin ligase complex to methylated target proteins (PubMed : 29691401, PubMed : 30442713). Required for normal maturation of myeloid progenitor cells (By similarity).
See full target information L3MBTL3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e13222 PubMed36747531

2023

L3MBTL3 is induced by HIF-1α and fine tunes the HIF-1α degradation under hypoxia in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Mengdong Wang,Di Wang,Yue Lang,Anwen Shao,Rui Zhang,Jun Tang,Dongming Lai,Chenglu Xiao

Breast cancer research : BCR 14:R63 PubMed22513257

2012

Effects of BRCA2 cis-regulation in normal breast and cancer risk amongst BRCA2 mutation carriers.

Applications

Unspecified application

Species

Unspecified reactive species

Ana-Teresa Maia,Antonis C Antoniou,Martin O'Reilly,Shamith Samarajiwa,Mark Dunning,Christiana Kartsonaki,Suet-Feung Chin,Christina N Curtis,Lesley McGuffog,Susan M Domchek,Douglas F Easton,Susan Peock,Debra Frost,D Gareth Evans,Ros Eeles,Louise Izatt,Julian Adlard,Diana Eccles,Olga M Sinilnikova,Sylvie Mazoyer,Dominique Stoppa-Lyonnet,Marion Gauthier-Villars,Laurence Faivre,Laurence Venat-Bouvet,Capucine Delnatte,Heli Nevanlinna,Fergus J Couch,Andrew K Godwin,Maria Adelaide Caligo,Rosa B Barkardottir,Xiaoqing Chen,Jonathan Beesley,Sue Healey,Carlos Caldas,Georgia Chenevix-Trench,Bruce A J Ponder
View all publications
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Product promise

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