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AB99928

Anti-L3MBTL3 antibody

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(2 Publications)

Rabbit Polyclonal L3MBTL3 antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human L3MBTL3 aa 550-600.

View Alternative Names

KIAA1798, MBT1, L3MBTL3, Lethal(3)malignant brain tumor-like protein 3, H-l(3)mbt-like protein 3, L(3)mbt-like protein 3, L3mbt-like 3, MBT-1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L3MBTL3 antibody (AB99928)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L3MBTL3 antibody (AB99928)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling L3MBTL3 with ab99928 at 1/1000 (1µg/ml). Detection : Peroxidase Substrate.

Immunoprecipitation - Anti-L3MBTL3 antibody (AB99928)
  • IP

Unknown

Immunoprecipitation - Anti-L3MBTL3 antibody (AB99928)

Immunoprecipitation analysis of HeLa whole cell lysate (1mg/IP; 20% of IP loaded/lane) using ab99928 at 3μg/mg lysate. A rabbit anti-human L3MBTL3 was used for the western blot at 1μg/ml.

Left Lane - ab99928 IP, Right Lane - control IgG.

All lanes:

Immunoprecipitation - Anti-L3MBTL3 antibody (ab99928)

Predicted band size: 88 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human L3MBTL3 aa 550-600. The exact immunogen used to generate this antibody is proprietary information.

Q96JM7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L3MBTL3 also known as Lethal(3) Malignant Brain Tumor-Like Protein 3 is a member of the polycomb group proteins which regulate gene expression by remodeling chromatin. It has a molecular weight of approximately 69 kDa. L3MBTL3 is widely expressed in various tissues with significant levels in the brain and hematopoietic cells. This protein plays a role in chromatin compaction through its ability to recognize and bind mono- and dimethylated lysine residues on histones. These activity patterns classify it as a histone reader influencing the accessibility of transcriptionally active regions.
Biological function summary

L3MBTL3 affects cellular processes by managing chromatin structure which in turn regulates gene expression. It participates in chromatin remodeling as part of large multi-protein complexes. By interacting with different components L3MBTL3 modulates the transcription of oncogenes and tumor suppressors. It contributes to important cellular processes including cell-cycle progression and differentiation by establishing particular transcriptional profiles. The protein's role in these processes highlights its importance in maintaining normal cell function.

Pathways

L3MBTL3 participates in pathways that involve chromatin modification and gene silencing. It is associated with the Polycomb Repressive Complex 2 (PRC2) pathway which plays a role in maintaining transcriptional repression. This pathway involves key proteins such as EZH2 and SUZ12 which work alongside L3MBTL3 to modify histone marks thereby silencing target genes. This positioning within pathways makes it an integral component of transcriptional regulation by ensuring genes are restricted as necessary for proper development and cellular activity.

L3MBTL3 has associations with cancer and hematological abnormalities. Its dysregulation has links to certain types of leukemia where abnormal expression can lead to uncontrolled cell proliferation. Another condition tied to L3MBTL3 is brain malignancies due to perturbations in its normal function within neuronal cells. Interactions with proteins like EZH2 further connect L3MBTL3 to cancer pathways emphasizing the relevance of its careful regulation in maintaining cellular health and preventing disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Is a negative regulator of Notch target genes expression, required for RBPJ-mediated transcriptional repression (PubMed : 29030483). It recruits KDM1A to Notch-responsive elements and promotes KDM1A-mediated H3K4me demethylation (PubMed : 29030483). Involved in the regulation of ubiquitin-dependent degradation of a set of methylated non-histone proteins, including SOX2, DNMT1 and E2F1. It acts as an adapter recruiting the CRL4-DCAF5 E3 ubiquitin ligase complex to methylated target proteins (PubMed : 29691401, PubMed : 30442713). Required for normal maturation of myeloid progenitor cells (By similarity).
See full target information L3MBTL3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:231 PubMed39747894

2025

L3MBTL3 and STAT3 collaboratively upregulate SNAIL expression to promote metastasis in female breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jianpeng Xiao,Jie Wang,Jialun Li,Jie Xiao,CuiCui Liu,Libi Tan,Yanhong Tu,Ruifang Yang,Yujie Pei,Minghua Wang,Jiemin Wong,Binhua P Zhou,Jing Li,Jing Feng

Gut 68:693-707 PubMed30415234

2018

Stromal protein βig-h3 reprogrammes tumour microenvironment in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Delphine Goehrig,Jérémy Nigri,Rémi Samain,Zhichong Wu,Paola Cappello,Gaëlle Gabiane,Xinyi Zhang,Yajie Zhao,In-San Kim,Marie Chanal,Roberta Curto,Valerie Hervieu,Christelle de La Fouchardière,Francesco Novelli,Pascale Milani,Richard Tomasini,Corinne Bousquet,Philippe Bertolino,Ana Hennino
View all publications
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Product promise

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