Anti-L3MBTL3 antibody [EPR11854] - BSA and Azide free

• WB

Unknown

Western blot - Anti-L3MBTL3 antibody [EPR11854] - BSA and Azide free (AB249882)

This data was developed using ab175232, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab175232 observed at 88 kDa. Red - loading control ab8245 observed at 36 kDa.

ab175232 Anti-L3MBTL3 antibody [EPR11854] was shown to specifically react with L3MBTL3 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266172 (knockout cell lysate ab258489) was used. Wild-type and L3MBTL3 knockout samples were subjected to SDS-PAGE. ab175232 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-L3MBTL3 antibody [EPR11854] (<a href='/en-us/products/primary-antibodies/l3mbtl3-antibody-epr11854-ab175232'>ab175232</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

L3MBTL3 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

Western blot - Human L3MBTL3 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-l3mbtl3-knockout-hek-293t-cell-line-ab266172'>ab266172</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 88 kDa

Observed band size: 88 kDa

false

• WB

Supplier Data

Western blot - Anti-L3MBTL3 antibody [EPR11854] - BSA and Azide free (AB249882)

This data was developed using ab175232, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-L3MBTL3 antibody [EPR11854] (<a href='/en-us/products/primary-antibodies/l3mbtl3-antibody-epr11854-ab175232'>ab175232</a>) at 1/1000 dilution

Lane 1:

HeLa lysate at 10 µg

Lane 2:

293T lysate at 10 µg

Lane 3:

Human fetal brain lysate at 10 µg

Predicted band size: 88 kDa

false