JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317641

Anti-Lactate Dehydrogenase A (LDHA) antibody [EPR25915-88] - BSA and Azide free (Detector)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal LDHA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M

1 Images
Sandwich ELISA - Anti-Lactate Dehydrogenase A (LDHA) antibody [EPR25915-88] - BSA and Azide free (Detector) (AB317641)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Lactate Dehydrogenase A (LDHA) antibody [EPR25915-88] - BSA and Azide free (Detector) (AB317641)

Sandwich ELISA of ab317639 with the capture antibody ab317640 dilution at 2 ug/mL and detector antibody ab317641 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25915-88

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab317641 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Lactate Dehydrogenase A (LDHA). The recommended pair for sandwich ELISA is:

Capture: ab317640, Human Lactate Dehydrogenase A (LDHA) Capture Antibody (unconjugated)

Detector: ab317641, Human Lactate Dehydrogenase A (LDHA) Detector Antibody (unconjugated)

The reference range value is 39.06-5000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISA based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate Dehydrogenase A (LDHA) also called LDH-1 or LDH-M is an enzyme that plays an important role in the conversion of pyruvate to lactate while converting NADH to NAD+. LDHA is a tetrameric protein with a molecular mass of approximately 36 kDa per subunit. It is expressed in various tissues but it is especially abundant in skeletal muscle and the liver. The high expression of LDHA allows these tissues to efficiently perform anaerobic glycolysis especially under low oxygen conditions.
Biological function summary

LDHA facilitates the last step of anaerobic glycolysis to regenerate the essential cofactor NAD+ which is necessary for maintaining glycolytic flux. This enzyme operates as part of a multienzyme complex collaborating with other glycolytic enzymes to ensure efficient metabolic processes. In rapidly growing cells like cancer cells LDHA supports the high demand for energy and biosynthetic precursors by promoting the conversion of pyruvate to lactate. The activity of LDHA is an important adaptation mechanism in cells experiencing stress or rapid proliferation.

Pathways

LDHA is significant in the glycolysis and gluconeogenesis pathways impacting cellular energy production and carbon flow. In glycolysis it works with other enzymes like hexokinase and phosphofructokinase to convert glucose to lactate especially under anaerobic conditions. LDHA also interacts with proteins such as lactate dehydrogenase B (LDHB) that facilitate the conversion of lactate back to pyruvate in tissues that prefer aerobic metabolism. This balance between LDHA-driven lactate production and LDHB-driven lactate utilization is important for maintaining energy homeostasis in various tissues.

LDHA is frequently linked to cancer and metabolic disorders. In many solid tumors LDHA expression is upregulated meeting the energetic and biosynthetic demands of cancerous cells and contributing to the Warburg effect where cancer cells prefer glycolysis over oxidative phosphorylation even in the presence of oxygen. Additionally LDHA's interaction with oncogenes such as MYC can amplify its expression supporting tumorigenesis. In metabolic disorders like lactic acidosis dysregulation of LDHA can result in excessive lactate production leading to harmful acid levels in the blood. Understanding LDHA's role in these diseases can guide therapeutic strategies targeting metabolic pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
See full target information LDHA
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB102526

LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric)

Biochemical assay - LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (AB102526)

  • 4
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB209847

Anti-Hexokinase II antibody [EPR20839]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Hexokinase II antibody [EPR20839] (AB209847)

  • 5
  • 2
Primary Antibodies

AB52488

Anti-Lactate Dehydrogenase antibody [EP1566Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase antibody [EP1566Y] (AB52488)

  • 4
  • 7

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com