AB135396

Anti-Lactate Dehydrogenase antibody [98A-1F9BB1]

Name: Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) | Abcam
Brand: Abcam Limited
SKU: AB135396
Price: 485 USD
Availability: InStock

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(5 Publications)

Mouse Monoclonal LDHA antibody. Suitable for ICC, IP, Flow Cyt, WB, In-Cell ELISA and reacts with Human, Mouse, Rat, Rabbit samples. Cited in 5 publications.

View Alternative Names

PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M

5 Images

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Immunocytochemistry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (AB135396)

Immunocytochemistry analysis of Paraformaldehyde fixed Hela cells labelling Lactate Dehydrogenase with ab135396 at 1 μg/ml. Detection utilised Alexa 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution.

Flow Cytometry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (AB135396)

Flow Cyt

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Flow Cytometry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (AB135396)

Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labelling Lactate Dehydrogenase with ab135396 at 1 μg/ml (blue) or an equal amount of an isotype control antibody (red). 1% BSA was used as the blocking reagent for all the blocking steps.

Unknown

Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (AB135396)

Immunoprecipitation of Lactate Dehydrogenase from 1mg of Hela Cell Lysate with ab135396 at 1 μg/ml. The gel was stained with Coomassie brilliant blue G.

All lanes:

Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)

Predicted band size: 37 kDa

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In-Cell ELISA - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (AB135396)

In-Cell ELISA

Unknown

In-Cell ELISA - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (AB135396)

In-cell ELISA analysis of paraformaldehyde/PBS fixed HeLa cells seeded at 20k cells/well. ab135396 was serially diluted in 1%BSA/PBS and detected with goat anti-mouse dye-conjugated antibody at 1/1000 in 1%BSA/PBS.

Unknown

Western blot - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (AB135396)

The high background signal in mouse tissue sample was caused by the direct reaction between the mouse IgG in mouse tissue preps and the goat anti-mouse secondary antibody. LDHA and LDHB are resolved in tissues containing both isoforms.

All lanes:

Western blot - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) at 1 µg/mL

Lanes 1 and 8:

Ladder

Lane 2:

Human liver homogenate at 15 µg

Lane 3:

Human heart homogenate at 15 µg

Lane 4:

Rat liver homogenate at 15 µg

Lane 5:

Rat heart homogenate at 15 µg

Lane 6:

Mouse liver homogenate at 15 µg

Lane 7:

Mouse heart homogenate at 15 µg

Lane 9:

Human fibroblast whole cell lysate at 15 µg

Lane 10:

HeLa whole Cell Lysate at 15 µg

Lane 11:

HepG2 whole cell lysate at 15 µg

Lane 12:

H9C2 (Rat cardiomyoblast cell line) whole Cell Lysate at 15 µg

Lane 13:

H4IIE (Rat hepatoma cell line) whole Cell Lysate at 15 µg

Lane 14:

MEF (Mouse embryonic fibroblast cell line) whole Cell Lysate at 15 µg

Lane 15:

LDH (purified from rabbit skeletal muscle) at 0.015 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - AP at 1/3000 dilution

Predicted band size: 37 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

98A-1F9BB1

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Rabbit, Human

Applications

Flow Cyt, In-Cell ELISA, IP, ICC, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "InCellELISA" : {"fullname" : "In-Cell ELISA", "shortname":"In-Cell ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1 µg/mL", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "InCellELISA-species-checked": "testedAndGuaranteed", "InCellELISA-species-dilution-info": "1 µg/mL", "InCellELISA-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "InCellELISA-species-checked": "guaranteed", "InCellELISA-species-dilution-info": "", "InCellELISA-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "InCellELISA-species-checked": "guaranteed", "InCellELISA-species-dilution-info": "", "InCellELISA-species-notes": "" }, "Cow": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1 µg/mL", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "InCellELISA-species-checked": "notRecommended", "InCellELISA-species-dilution-info": "1 µg/mL", "InCellELISA-species-notes": "" }, "Rabbit": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "InCellELISA-species-checked": "guaranteed", "InCellELISA-species-dilution-info": "", "InCellELISA-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.877% Sodium chloride, 0.357% HEPES

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate along with the conversion of NADH to NAD+. LDH is known by other names such as lactic acid dehydrogenase and LDH-5. The enzyme has a molecular weight of approximately 36 kDa. LDH exists in almost all tissues having multiple isoforms that are expressed differently depending on the tissue type. It shows high expression in muscle tissue liver and heart indicating its extensive role in energy metabolism.

Biological function summary

Lactate dehydrogenase plays a critical role in anaerobic glycolysis. The enzyme helps in regenerating NAD+ from NADH allowing glycolysis to continue in the absence of oxygen. LDH is not a part of any larger protein complex working independently to fulfill its function in the glycolytic pathway. It serves in rapid energy production especially under hypoxic or exertional conditions where oxygen supply is limited.

Pathways

LDH is significantly involved in the glycolysis and gluconeogenesis pathways. Within glycolysis LDH helps facilitate the conversion of pyruvate to lactate during anaerobic conditions a step important for ATP production when oxygen is scarce. The enzyme is tied closely to phosphofructokinase-1 (PFK-1) in glycolysis given that both enzymes are central to maintaining the glycolytic flow. In gluconeogenesis though functionally reversed from its role in glycolysis LDH helps to manage lactate removal an important step for glucose synthesis from non-carbohydrate sources.

Lactate dehydrogenase levels often act as a biomarker for tissue damage or certain cancers as its release into the bloodstream signals cellular injury or death. Elevated LDH levels are associated with conditions like myocardial infarction and certain forms of anemia. In cancer such as lymphoma or leukemia LDH correlates with the progression of the disease and acts as a prognostic marker. LDH's connection to these conditions often leads to insights into disease severity and progression due to its association with proteins like p53 and HIF-1 which play roles in cellular metabolism and hypoxia response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

See full target information LDHA

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 3:954-968 PubMed34226744

2021

Enzymatic activation of pyruvate kinase increases cytosolic oxaloacetate to inhibit the Warburg effect.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth K Wiese,Sadae Hitosugi,Sharon T Loa,Annapoorna Sreedhar,Lindsey G Andres-Beck,Kiran Kurmi,Yuan-Ping Pang,Larry M Karnitz,Wilson I Gonsalves,Taro Hitosugi

Journal of neurochemistry 158:482-499 PubMed33905537

2021

Nuclear lipidome is altered in amyotrophic lateral sclerosis: A pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Omar Ramírez-Nuñez,Mariona Jové,Pascual Torres,Joaquim Sol,Laia Fontdevila,Ricardo Romero-Guevara,Pol Andrés-Benito,Victòria Ayala,Chiara Rossi,Jordi Boada,Mònica Povedano,Isidro Ferrer,Reinald Pamplona,Manuel Portero-Otin

The Journal of physiology 593:4677-88 PubMed26282186

2015

Haematological rather than skeletal muscle adaptations contribute to the increase in peak oxygen uptake induced by moderate endurance training.

Applications

Unspecified application

Species

Unspecified reactive species

David Montero,Adrian Cathomen,Robert A Jacobs,Daniela Flück,Jeroen de Leur,Stefanie Keiser,Thomas Bonne,Niels Kirk,Anne-Kristine Lundby,Carsten Lundby

The Journal of physiology 594:1151-66 PubMed26339730

2015

Twenty-eight days of exposure to 3454 m increases mitochondrial volume density in human skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Robert A Jacobs,Anne-Kristine Meinild Lundby,Simone Fenk,Saskia Gehrig,Christoph Siebenmann,Daniela Flück,Niels Kirk,Matthias P Hilty,Carsten Lundby

PloS one 10:e0129303 PubMed26121149

2015

Gene Expression Profiling of H9c2 Myoblast Differentiation towards a Cardiac-Like Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Ana F Branco,Susana P Pereira,Susana Gonzalez,Oleg Gusev,Albert A Rizvanov,Paulo J Oliveira

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lactate Dehydrogenase antibody [98A-1F9BB1]

Immunocytochemistry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (AB135396)

Flow Cytometry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (AB135396)

Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (AB135396)

In-Cell ELISA - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (AB135396)

Western blot - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (AB135396)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise