AB231903

Anti-Lactate Dehydrogenase antibody

Name: Anti-Lactate Dehydrogenase antibody (ab231903) | Abcam
Brand: Abcam Limited
SKU: AB231903
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal LDHA antibody. Suitable for WB, IHC-P and reacts with Pig, Recombinant full length protein - Pig, Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Pig LDHA.

View Alternative Names

PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M

4 Images

Supplier Data

Western blot - Anti-Lactate Dehydrogenase antibody (AB231903)

All lanes:

Western blot - Anti-Lactate Dehydrogenase antibody (ab231903) at 3 µg/mL

Lane 1:

Porcine serum

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate

Lane 3:

A-375 (human malignant melanoma cell line) whole cell lysate

Lane 4:

Porcine kidney tissue lysate

Lane 5:

Mouse testis tissue lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody (AB231903)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody (AB231903)

Formalin-fixed, paraffin-embedded porcine skeletal muscle tissue stained for Lactate Dehydrogenase using ab231903 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody (AB231903)

Formalin-fixed, paraffin-embedded porcine heart tissue stained for Lactate Dehydrogenase using ab231903 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Lactate Dehydrogenase antibody (AB231903)

All lanes:

Western blot - Anti-Lactate Dehydrogenase antibody (ab231903) at 3 µg/mL

All lanes:

Recombinant porcine Lactate Dehydrogenase protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 67 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Pig LDHA.

P00339

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate along with the conversion of NADH to NAD+. LDH is known by other names such as lactic acid dehydrogenase and LDH-5. The enzyme has a molecular weight of approximately 36 kDa. LDH exists in almost all tissues having multiple isoforms that are expressed differently depending on the tissue type. It shows high expression in muscle tissue liver and heart indicating its extensive role in energy metabolism.

Biological function summary

Lactate dehydrogenase plays a critical role in anaerobic glycolysis. The enzyme helps in regenerating NAD+ from NADH allowing glycolysis to continue in the absence of oxygen. LDH is not a part of any larger protein complex working independently to fulfill its function in the glycolytic pathway. It serves in rapid energy production especially under hypoxic or exertional conditions where oxygen supply is limited.

Pathways

LDH is significantly involved in the glycolysis and gluconeogenesis pathways. Within glycolysis LDH helps facilitate the conversion of pyruvate to lactate during anaerobic conditions a step important for ATP production when oxygen is scarce. The enzyme is tied closely to phosphofructokinase-1 (PFK-1) in glycolysis given that both enzymes are central to maintaining the glycolytic flow. In gluconeogenesis though functionally reversed from its role in glycolysis LDH helps to manage lactate removal an important step for glucose synthesis from non-carbohydrate sources.

Lactate dehydrogenase levels often act as a biomarker for tissue damage or certain cancers as its release into the bloodstream signals cellular injury or death. Elevated LDH levels are associated with conditions like myocardial infarction and certain forms of anemia. In cancer such as lymphoma or leukemia LDH correlates with the progression of the disease and acts as a prognostic marker. LDH's connection to these conditions often leads to insights into disease severity and progression due to its association with proteins like p53 and HIF-1 which play roles in cellular metabolism and hypoxia response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

See full target information LDHA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 49: PubMed36704851

2023

miR‑151a‑5p promotes the proliferation and metastasis of colorectal carcinoma cells by targeting AGMAT.

Applications

Unspecified application

Species

Unspecified reactive species

Yaya Xie,Yue Zhang,Xianju Liu,Lijun Cao,Mengting Han,Chunmei Wang,Jinlian Chen,Xingxing Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lactate Dehydrogenase antibody

Western blot - Anti-Lactate Dehydrogenase antibody (AB231903)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody (AB231903)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody (AB231903)

Western blot - Anti-Lactate Dehydrogenase antibody (AB231903)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise