Anti-Lactate Dehydrogenase antibody [EP1566Y] - BSA and Azide free

9 product images

• 

  Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [EP1566Y] - BSA and Azide free (ab219591)

  Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488 immunoprecipitating Lactate Dehydrogenase. 10μg of cell lysate was incubated with primary antibody at a dilution of 1/30 and VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at a dilution of 1/10000.
  

  Lane 1: HeLa (human cervix adenocarcinoma) whole cell lysate (10ug)
  Lane 2: HeLa (human cervix adenocarcinoma) whole cell lysate
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488 in HeLa (human cervix adenocarcinoma) whole cell lysate

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488).

  All lanes: Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [EP1566Y] (Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488)

  Predicted band size: 37 kDa

  Observed band size: 36 kDa

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase antibody [EP1566Y] - BSA and Azide free (ab219591)

  Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488 staining Lactate Dehydrogenase in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 were used as counterstains for primary antibody Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488 and secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 respectively and DAPI was used as a nuclear counterstain.

  Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120)
  Negative control 2: Mouse primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) and anti-rabbit secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] - BSA and Azide free (ab219591)

  Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488 staining Lactate Dehydrogenase in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

  Negative control 1: PBS in place of primary antibody.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] - BSA and Azide free (ab219591)

  Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488 staining Lactate Dehydrogenase in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

  Negative control 1: PBS in place of primary antibody.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] - BSA and Azide free (ab219591)

  Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488 staining Lactate Dehydrogenase in human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.

  Negative control 1: PBS in place of primary antibody.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488).

• 

  Flow Cytometry (Intracellular) - Anti-Lactate Dehydrogenase antibody [EP1566Y] - BSA and Azide free (ab219591)

  Intracellular Flow Cytometry analysis of Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate labelling Lactate Dehydrogenase with purified Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488 at 1/190 (red). Cells were fixed with 4% paraformaldehyde. Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] - BSA and Azide free (ab219591)

  Immunohistochemical analysis of paraffin-embedded human liver carcinoma using unpurified Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488 at a 1/50 dilution.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase antibody [EP1566Y] - BSA and Azide free (ab219591)

  ICC/IF image of unpurified Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488).

• 

  Flow Cytometry (Intracellular) - Anti-Lactate Dehydrogenase antibody [EP1566Y] - BSA and Azide free (ab219591)

  Overlay histogram showing HeLa cells stained with unpurified Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Lactate Dehydrogenase antibody [EP1566Y] ab52488).