AB101562

Anti-Lactate Dehydrogenase antibody [EPR1564]

Name: Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562) | Abcam
Brand: Abcam Limited
SKU: AB101562
Price: 330 USD
Availability: InStock
Rating: 4 (3 reviews)

RabMAb
Recombinant
What is this?

(3 Reviews)

(92 Publications)

Rabbit Recombinant Monoclonal LDHA antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 92 publications.

View Alternative Names

PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EPR1564] (AB101562)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EPR1564] (AB101562)

Staining of Human Lactate Dehydrogenase in a Formalin/PFA-fixed paraffin-embedded section of human Muscle using ab101526 at a dilution of 1/500.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Lactate Dehydrogenase antibody [EPR1564] (AB101562)

All lanes:

Western blot - Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

A375 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Predicted band size: 37 kDa

false

Carrier free

Anti-Lactate Dehydrogenase antibody [EPR1564] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1564

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen for this antibody has 93% homology with L-lactate dehydrogenase C chain (P07864).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Antigen retrieval is strongly recommended.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate dehydrogenase (LDH) is an enzyme that catalyzes the interconversion of pyruvate and lactate along with the conversion of NADH to NAD+. LDH is known by other names such as lactic acid dehydrogenase and LDH-5. The enzyme has a molecular weight of approximately 36 kDa. LDH exists in almost all tissues having multiple isoforms that are expressed differently depending on the tissue type. It shows high expression in muscle tissue liver and heart indicating its extensive role in energy metabolism.

Biological function summary

Lactate dehydrogenase plays a critical role in anaerobic glycolysis. The enzyme helps in regenerating NAD+ from NADH allowing glycolysis to continue in the absence of oxygen. LDH is not a part of any larger protein complex working independently to fulfill its function in the glycolytic pathway. It serves in rapid energy production especially under hypoxic or exertional conditions where oxygen supply is limited.

Pathways

LDH is significantly involved in the glycolysis and gluconeogenesis pathways. Within glycolysis LDH helps facilitate the conversion of pyruvate to lactate during anaerobic conditions a step important for ATP production when oxygen is scarce. The enzyme is tied closely to phosphofructokinase-1 (PFK-1) in glycolysis given that both enzymes are central to maintaining the glycolytic flow. In gluconeogenesis though functionally reversed from its role in glycolysis LDH helps to manage lactate removal an important step for glucose synthesis from non-carbohydrate sources.

Lactate dehydrogenase levels often act as a biomarker for tissue damage or certain cancers as its release into the bloodstream signals cellular injury or death. Elevated LDH levels are associated with conditions like myocardial infarction and certain forms of anemia. In cancer such as lymphoma or leukemia LDH correlates with the progression of the disease and acts as a prognostic marker. LDH's connection to these conditions often leads to insights into disease severity and progression due to its association with proteins like p53 and HIF-1 which play roles in cellular metabolism and hypoxia response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

See full target information LDHA

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Publications (92)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:322 PubMed41024119

2025

Transcription factor MYB regulates glycolysis to promote the transfer and malignant progression of gastric cancer through the SSBP2/ISL1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenni Sun,Mi Zhou,Yasai Yao,Xuehong Chen,Tao Qin,Yafei Han,Lu Yue,Ruyong Yao

Cell death and differentiation : PubMed40830246

2025

Intestinal metabolite TMAO promotes CKD progression by stimulating macrophage M2 polarization through histone H4 lysine 12 lactylation.

Applications

Unspecified application

Species

Unspecified reactive species

Youzhou Tang,Yuxin Li,Xinyu Yang,Tianze Lu,Xinran Wang,Zhi Li,Jun Liu,Jianwen Wang

Oncoimmunology 13:2320951 PubMed38419759

2024

Lactate/GPR81 recruits regulatory T cells by modulating CX3CL1 to promote immune resistance in a highly glycolytic gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Su,Xinyuan Mao,Lingzhi Wang,Zhian Chen,Weisheng Wang,Cuiyin Zhao,Guoxin Li,Weihong Guo,Yanfeng Hu

International journal of biological sciences 20:516-536 PubMed38169635

2024

p32 regulates glycometabolism and TCA cycle to inhibit ccRCC progression via copper-induced DLAT lipoylation oligomerization.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoping Tian,Rui Wang,Yiting Wang,Ruibing Chen,Tianyu Lin,Xuesong Xiao,Xinyu Liu,Justin Eze Ideozu,Hua Geng,Yong Wang,Dan Yue

Journal of cellular and molecular medicine 28:e18059 PubMed38140828

2023

Pirfenidone inhibits TGF-β1-induced metabolic reprogramming during epithelial-mesenchymal transition in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shuling Zhang,Yuanmei Wang,Daiqin Luo,Zhimei Cheng,Qibing Zeng,Guoze Wang,Mengxue Chen,Shuai Zhang,Peng Luo

Journal of biochemical and molecular toxicology 37:e23406 PubMed37392398

2023

CircDUS2L (circ_0039908) promotes lung adenocarcinoma progression by upregulating PGAM1 by acting as a miR-590-5p molecular sponge.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Tian,Ying Guo,Jinfeng Liu,Chao Pang,Qiang Wang,Qi Xie,Jianke Li

Journal of translational internal medicine 12:437-451 PubMed39360158

2023

Inhibition of heterogeneous nuclear ribonucleoproteins A1 and oxidative stress reduces glycolysis pyruvate kinase M2 in chronic thromboembolic pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Lianhua Liu,Wenyi Pang,Jixiang Liu,Shiqing Xu,Zhu Zhang,Risheng Hao,Jun Wan,Wanmu Xie,Xincao Tao,Peiran Yang,Lan Zhao,Zhenguo Zhai,Chen Wang

Clinical and experimental pharmacology & physiology 50:463-475 PubMed36809521

2023

Hsa_circ_0000591 drives osteosarcoma glycolysis and progression by sequestering miR-194-5p and elevating HK2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Dawei Wang,Yang Wang,Huadong Wang,Yafeng Yang,Li Li,Yihao Liu,Xin Yin

Molecular medicine reports 27: PubMed36734266

2023

Shikonin improves pulmonary vascular remodeling in monocrotaline‑induced pulmonary arterial hypertension via regulation of PKM2.

Applications

Unspecified application

Species

Unspecified reactive species

Wenfeng Li,Wenjuan Chen,Hongyan Peng,Zhenghui Xiao,Jinqiao Liu,Yunhong Zeng,Ting Huang,Qingqing Song,Xun Wang,Yunbin Xiao

International journal of molecular sciences 23: PubMed36555289

2022

3-Bromopyruvate Suppresses the Malignant Phenotype of Vemurafenib-Resistant Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Patrik da Silva Vital,Murilo Bonatelli,Marina Pereira Dias,Larissa Vedovato Vilela de Salis,Mariana Tomazini Pinto,Fátima Baltazar,Silvya Stuchi Maria-Engler,Céline Pinheiro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lactate Dehydrogenase antibody [EPR1564]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EPR1564] (AB101562)

Western blot - Anti-Lactate Dehydrogenase antibody [EPR1564] (AB101562)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (92)

Product promise