Goat Polyclonal L-lactate dehydrogenase B antibody. Suitable for WB and reacts with Pig, Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LDHB aa 250 to C-terminus.
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Publications
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- International journal of molecular sciences 24:2023Polymer-Functionalized Mitochondrial Transplantation to Fibroblasts Counteracts a Pro-Fibrotic Phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGherardo Baudo,Suhong Wu,Matteo Massaro,Haoran Liu,Hyunho Lee,Aijun Zhang,Dale J Hamilton,Elvin BlancoPubMed 37446100
- Oncology letters 18:1431-14412019Correlation and expression analysis of hypoxia-inducible factor 1α, glucose transporter 1 and lactate dehydrogenase 5 in human gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLang-Song Hao et. al.PubMed 31423208
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LDHB aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P07195
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
| Chimpanzee | Predicted |
| Cow | Predicted |
| Cynomolgus monkey | Predicted |
| Dog | Predicted |
| Pig | Tested |
| Rabbit | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info 0.03000-0.10000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Mouse | Dilution info 0.30000-1.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Rat | Dilution info 0.30000-1.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Human | Dilution info 0.03000-0.10000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Dog, Chimpanzee, Cynomolgus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Alternative names
L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, Renal carcinoma antigen NY-REN-46, LDH-H, LDHB
Recommended products
Goat Polyclonal L-lactate dehydrogenase B antibody. Suitable for WB and reacts with Pig, Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LDHB aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LDHB aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P07195
- Purification technique
- Affinity purification Immunogen
- Specificity
Reported variants represent identical protein: NP_002291.1, NP_001167568.1.
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lactate Dehydrogenase B (LDH-B) also known as LDH is an enzyme that plays an important role in the interconversion of lactate to pyruvate. It belongs to the lactate dehydrogenase family which includes multiple isoenzymes. LDH-B has a molecular weight of approximately 36 kDa and commonly forms a tetramer with three other similar subunits. It is mainly expressed in heart and muscle tissues among others where it functions effectively.
- Biological function summary
LDH-B is essential for cellular energy production processes. It catalyzes the conversion of pyruvate a byproduct of glycolysis into lactate while simultaneously converting NADH to NAD+. This reaction is significant under anaerobic conditions where mitochondria-based ATP production is limited. LDH-B does not function alone and can operate as part of the lactate dehydrogenase complex containing different subunit combinations.
- Pathways
Lactate and pyruvate metabolism engage LDH-B facilitating the Cori cycle and the lactate shuttle. These pathways enable lactate transport between tissues such as muscle and liver and they are important in maintaining blood glucose levels and energy balance. LDH-B operates alongside its related isoenzymes such as LDH-A which predominantly act in specific tissues and conditions to optimize energy utilization.
- Associated diseases and disorders
Anomalous LDH-B activity associates with conditions like myocardial infarction and some cancer types. Enhanced expression or activity of LDH-B can indicate altered metabolic states in tumors connecting it as a potential marker for certain cancerous conditions. The relationship with other proteins such as the hypoxia-inducible factor (HIF) is notable especially in cancer where HIF can regulate LDH-B expression under low oxygen environments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482)
Primary incubation for 1 hour.
All lanes: Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482) at 0.1 µg/mL
All lanes: Pig heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482)
Primary incubation for 1 hour.
All lanes: Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482) at 0.1 µg/mL
Lane 1: Human heart lysate in RIPA buffer at 35 µg
Lane 2: Mouse heart lysate in RIPA buffer at 35 µg
Lane 3: Rat heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 21 kDa, 37 kDa
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