AB240482

Anti-Lactate Dehydrogenase B/LDH-B antibody

Name: Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482) | Abcam
Brand: Abcam Limited
SKU: AB240482

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(5 Publications)

Goat Polyclonal L-lactate dehydrogenase B antibody. Suitable for WB and reacts with Pig, Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human LDHB aa 250 to C-terminus.

View Alternative Names

L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, Renal carcinoma antigen NY-REN-46, LDH-H, LDHB

2 Images

Supplier Data

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (AB240482)

Primary incubation for 1 hour.

All lanes:

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482) at 0.1 µg/mL

Lane 1:

Human heart lysate in RIPA buffer at 35 µg

Lane 2:

Mouse heart lysate in RIPA buffer at 35 µg

Lane 3:

Rat heart lysate in RIPA buffer at 35 µg

Predicted band size: 21 kDa,37 kDa

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Supplier Data

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (AB240482)

Primary incubation for 1 hour.

All lanes:

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482) at 0.1 µg/mL

All lanes:

Pig heart lysate in RIPA buffer at 35 µg

Predicted band size: 37 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

applications

Immunogen

Synthetic Peptide within Human LDHB aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P07195

Specificity

Reported variants represent identical protein: NP_002291.1, NP_001167568.1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate Dehydrogenase B (LDH-B) also known as LDH is an enzyme that plays an important role in the interconversion of lactate to pyruvate. It belongs to the lactate dehydrogenase family which includes multiple isoenzymes. LDH-B has a molecular weight of approximately 36 kDa and commonly forms a tetramer with three other similar subunits. It is mainly expressed in heart and muscle tissues among others where it functions effectively.

Biological function summary

LDH-B is essential for cellular energy production processes. It catalyzes the conversion of pyruvate a byproduct of glycolysis into lactate while simultaneously converting NADH to NAD+. This reaction is significant under anaerobic conditions where mitochondria-based ATP production is limited. LDH-B does not function alone and can operate as part of the lactate dehydrogenase complex containing different subunit combinations.

Pathways

Lactate and pyruvate metabolism engage LDH-B facilitating the Cori cycle and the lactate shuttle. These pathways enable lactate transport between tissues such as muscle and liver and they are important in maintaining blood glucose levels and energy balance. LDH-B operates alongside its related isoenzymes such as LDH-A which predominantly act in specific tissues and conditions to optimize energy utilization.

Anomalous LDH-B activity associates with conditions like myocardial infarction and some cancer types. Enhanced expression or activity of LDH-B can indicate altered metabolic states in tumors connecting it as a potential marker for certain cancerous conditions. The relationship with other proteins such as the hypoxia-inducible factor (HIF) is notable especially in cancer where HIF can regulate LDH-B expression under low oxygen environments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

See full target information LDHB

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 15:415 PubMed39533309

2024

Lactylation of Hdac1 regulated by Ldh prevents the pluripotent-to-2C state conversion.

Applications

Unspecified application

Species

Unspecified reactive species

Qiman Dong,Xiaoqiong Yang,Lingling Wang,Qingye Zhang,Nannan Zhao,Shanshan Nai,Xiaoling Du,Lingyi Chen

iScience 27:110462 PubMed39104405

2024

SHP2 mutations promote glycolysis and inhibit apoptosis via PKM2/hnRNPK signaling in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhou,Zhuoyang Fan,Guodong He,Wei Zhang,Guowei Yang,Lechi Ye,Jianmin Xu,Rong Liu

International journal of molecular sciences 25: PubMed38732272

2024

Retinoic Acid-Mediated Control of Energy Metabolism Is Essential for Lung Branching Morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hugo Fernandes-Silva,Marco G Alves,Marcia R Garcez,Jorge Correia-Pinto,Pedro F Oliveira,Catarina C F Homem,Rute S Moura

International journal of molecular sciences 24: PubMed37446100

2023

Polymer-Functionalized Mitochondrial Transplantation to Fibroblasts Counteracts a Pro-Fibrotic Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Gherardo Baudo,Suhong Wu,Matteo Massaro,Haoran Liu,Hyunho Lee,Aijun Zhang,Dale J Hamilton,Elvin Blanco

Oncology letters 18:1431-1441 PubMed31423208

2019

Correlation and expression analysis of hypoxia-inducible factor 1α, glucose transporter 1 and lactate dehydrogenase 5 in human gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lang-Song Hao,Qi Liu,Chuan Tian,Dong-Xing Zhang,Bo Wang,Dong-Xu Zhou,Zhao-Peng Li,Zhi-Xiang Yuan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Lactate Dehydrogenase B/LDH-B antibody

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (AB240482)

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (AB240482)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise