Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] - BSA and Azide free (AB244228)

This data was developed using ab53292, the same antibody clone in a different buffer formulation.

Human heart muscle labeled with ab53292 at 1/100 - 1/250 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] - BSA and Azide free (AB244228)

This data was developed using ab53292, the same antibody clone in a different buffer formulation.

Lanes 1 - 4 : Merged signal (red and green). Green - ab53292 observed at 37 kDa. Red - loading control, ab130007 observed at 125 kDa.

ab53292 was shown to react with Lactate Dehydrogenase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255403 (knockout cell lysate ab263761) was used. Wild-type and Lactate Dehydrogenase knockout samples were subjected to SDS-PAGE. ab53292 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] - BSA and Azide free (ab244228) at 1/5000 dilution

Lane 1:

Wild-type Hap1 cell lysate at 20 µg

Lane 2:

LDHB knockout Hap1 cell lysate at 20 µg

Lane 3:

Wild-type HEK-293T cell lysate at 20 µg

Lane 4:

LDHB knockout HEK-293T cell lysate at 20 µg

Predicted band size: 37 kDa

false

• WB

Unknown

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] - BSA and Azide free (AB244228)

This data was developed using ab53292, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] - BSA and Azide free (ab244228)

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP

Predicted band size: 37 kDa

Observed band size: 37 kDa

false

• WB

Lab

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] - BSA and Azide free (AB244228)

This data was developed using ab53292, the same antibody clone in a different buffer formulation.

Lanes 1 - 3 : Merged signal (red and green). Green - ab53292 observed at 37 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab53292 was shown to specifically react with Lactate Dehydrogenase B in wild-type HAP1 cells as signal was lost in LDHB (Lactate Dehydrogenase B) knockout cells. Wild-type and LDHB (Lactate Dehydrogenase B) knockout samples were subjected to SDS-PAGE. ab53292 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (<a href='/en-us/products/primary-antibodies/lactate-dehydrogenase-b-ldh-b-antibody-ep1565y-ab53292'>ab53292</a>) at 1/50000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

LDHB (Lactate Dehydrogenase B) knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Predicted band size: 37 kDa

false