AB112996

Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9]

Name: Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] (ab112996) | Abcam
Brand: Abcam Limited
SKU: AB112996
Price: 170 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal L-lactate dehydrogenase B antibody. Suitable for IP, Flow Cyt, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, Renal carcinoma antigen NY-REN-46, LDH-H, LDHB, PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M

3 Images

Flow Cytometry - Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] (AB112996)

Flow Cyt

Unknown

Flow Cytometry - Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] (AB112996)

ab112996 at 1ug/ml staining Lactate Dehydrogenase B/LDH-B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).

Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] (AB112996)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] (AB112996)

ab112996 at 2ug/ml staining Lactate Dehydrogenase B/LDH-B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.

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Immunoprecipitation - Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] (AB112996)

The 36.6 kDa LDHB protein along with 36.7kDa LDHA protein were immunoprecipitated from Hela cell lysate with ab112996. The identities of the proteins were confirmed by mass spectrometry. This gel was stained with colloidal Coomassie blue G.

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Immunoprecipitation - Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] (ab112996)

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10E6AA9

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, IP, ICC/IF

applications

Specificity

Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg/mL", "FlowCyt-species-notes": "Triton permeabilization required.<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

ab112996 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%).

Storage buffer

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99.98% HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate dehydrogenase (LDH) consists largely of two subunits LDHA and LDHB also known as LDH-A and LDH-B. These subunits form tetramers in various combinations with the common forms being LDH-5 (A4) and LDH-1 (B4). LDHA consists of 332 amino acids and has a molecular mass of about 36 kiloDaltons while LDHB is of similar size. LDHA and LDHB are widely expressed in tissues with LDHA prevalent in muscle tissues and LDHB in heart tissues. Their primary function is to catalyze the interconversion of pyruvate to L-lactate and vice-versa critical in anaerobic glycolysis.

Biological function summary

These LDH subunits play an important role in energy production under low oxygen conditions by allowing glycolysis to continue. The LDHA and LDHB subunits associate to form the active enzyme complex which can exist in different isoforms based on tissue-specific expression. This enzyme enables cells in oxygen-deprived conditions such as in muscles during extensive exercise or in tumors to regenerate NAD+ from NADH supporting anaerobic metabolic pathways.

Pathways

LDHA and LDHB are key players in the glycolytic pathway and gluconeogenesis. They regulate the conversion between pyruvate and lactate impacting the Cori cycle where lactate is transported to the liver for glucose production. This step links them to other metabolic pathways and proteins like the pyruvate kinase enzyme and the tricarboxylic acid cycle intermediates facilitating energy production.

Altered LDHA and LDHB expression links to conditions such as cancer and ischemic tissue damage. Many tumors exhibit increased LDHA expression supporting rapid energy production via anaerobic glycolysis. In cardiac disorders high LDHB levels can indicate heart damage such as a myocardial infarction. Both LDHA and LDHB are therefore important markers and potential therapeutic targets in these disease contexts interacting closely with proteins like hexokinase and glucose transporters.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).

See full target information LDHB

Additional targets

LDHA

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:474-482 PubMed40836094

2025

Quantitative imaging of lipid transport in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan M Iglesias-Artola,Kristin Böhlig,Kai Schuhmann,Katelyn C Cook,H Mathilda Lennartz,Milena Schuhmacher,Pavel Barahtjan,Cristina Jiménez López,Radek Šachl,Vannuruswamy Garikapati,Karina Pombo-Garcia,Annett Lohmann,Petra Riegerová,Martin Hof,Björn Drobot,Andrej Shevchenko,Alf Honigmann,André Nadler

Hematological oncology 42:e3316 PubMed39526588

2024

Isocitrate dehydrogenase 2 mutation promotes cytarabine resistance in acute myeloid leukemia by Warburg effect.

Applications

Unspecified application

Species

Unspecified reactive species

Jinrong Yang,Zixu Wang,Kun Wu,Bo Nie,Liyin Li,Jingyan Ruan,Qiang Zhou,Yun Zeng,Mingxia Shi

Journal of oncology 2021:5529770 PubMed33986801

2021

miR-1224-3p Promotes Breast Cancer Cell Proliferation and Migration through PGM5-Mediated Aerobic Glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Ran,Yanan Zhang,Yajiao Shi,Jie Liu,Huayue Li,Lihua Ding,Qinong Ye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9]

Flow Cytometry - Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] (AB112996)

Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] (AB112996)

Immunoprecipitation - Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] (AB112996)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

Product promise