AB131171

Anti-LACTB antibody [EPR8502]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal LACTB antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

MRPL56, UNQ843/PRO1781, LACTB

2 Images

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Western blot - Anti-LACTB antibody [EPR8502] (AB131171)

All lanes:

Western blot - Anti-LACTB antibody [EPR8502] (ab131171) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human fetal heart cell lysate at 10 µg

Lane 4:

Human fetal muscle lysate at 10 µg

Secondary

All lanes:

Goat Anti-rabbit HRP at 1/2000 dilution

Predicted band size: 61 kDa

Observed band size: 55 kDa

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OI-RD Scanning - Anti-LACTB antibody [EPR8502] (AB131171)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-LACTB antibody [EPR8502] (AB131171)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8502

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LACTB protein also known as Serine beta-lactamase-like protein LACTB functions primarily as a serine beta-lactamase-like protein involved in lipid metabolism. It is a mitochondrial protein with a molecular mass of approximately 61 kDa. LACTB is highly expressed in tissues such as the liver and kidney where its role in metabolic and oxidative processes is significant.

Biological function summary

LACTB has a critical function in mitochondrial maintenance and regulation. This protein is a part of the inner mitochondrial membrane and contributes to the regulation of mitochondrial lipid composition and mitochondrial morphology. It does not form a complex but interacts with several mitochondrial proteins to exert its functions. By adjusting the lipid makeup of the mitochondria LACTB influences various metabolic processes and cellular homeostasis.

Pathways

LACTB plays a significant role in oxidative phosphorylation and lipid metabolism pathways. In these pathways it interacts with various proteins that oversee lipid synthesis and mitochondrial function such as cardiolipin and fatty acid synthase. These interactions support energy production and the structural integrity of mitochondria which are essential for proper cellular metabolism and energy homeostasis.

LACTB has connections to cancer and metabolic syndrome. In cancer the protein's regulatory functions on mitochondrial lipid composition impact tumor growth and proliferation. Anomalies in LACTB expression have been associated with altered cellular metabolism observed in some cancers. In metabolic syndrome LACTB influences metabolic pathways altered in this disorder. In these conditions proteins like mTOR (mechanistic target of rapamycin) and AMPK (AMP-activated protein kinase) may interact with LACTB affecting cellular growth and metabolism regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial serine protease that acts as a regulator of mitochondrial lipid metabolism (PubMed : 28329758). Acts by decreasing protein levels of PISD, a mitochondrial enzyme that converts phosphatidylserine (PtdSer) to phosphatidylethanolamine (PtdEtn), thereby affecting mitochondrial lipid metabolism (PubMed : 28329758). It is unclear whether it acts directly by mediating proteolysis of PISD or by mediating proteolysis of another lipid metabolism protein (PubMed : 28329758). Acts as a tumor suppressor that has the ability to inhibit proliferation of multiple types of breast cancer cells : probably by promoting decreased levels of PISD, thereby affecting mitochondrial lipid metabolism (PubMed : 28329758).

See full target information LACTB

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 37:154-168.e7 PubMed39561766

2024

Human genetics identify convergent signals in mitochondrial LACTB-mediated lipid metabolism in cardiovascular-kidney-metabolic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Shen Li,Hongbo Liu,Hailong Hu,Eunji Ha,Praveena Prasad,Brenita C Jenkins,Ujjalkumar Subhash Das,Sarmistha Mukherjee,Kyosuke Shishikura,Renming Hu,Daniel J Rader,Liming Pei,Joseph A Baur,Megan L Matthews,Garret A FitzGerald,Melanie R McReynolds,Katalin Susztak

Recent patents on anti-cancer drug discovery 19:188-198 PubMed38214358

2024

Human Chorionic Gonadotropin Regulates the Smad Signaling Pathway by Antagonizing TGF-β in Giant Cell Tumor of Bone.

Applications

Unspecified application

Species

Unspecified reactive species

Tangbing Xu,Shenglin Xu,Guangwen Ma,Jun Chang,Chi Zhang,Ping Zhou,Chao Wang,Pengfei Xu,Junjun Yang,Yong Hu,Yunfeng Wu

Aging 15:15213-15227 PubMed38149985

2023

A potential therapeutic approach for gastric cancer: inhibition of LACTB transcript 1.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Nie,Lihua Hu,Zhiqiang Yan,Yang Wang,Qianyun Shi,Shui He,Qian Wang,Fang Yang

Cancer science 114:1396-1409 PubMed36562402

2023

Circular RNA circ0104103 inhibits colorectal cancer progression through interactions with HuR and miR-373-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Pei Tan,Huiling Sun,Mu Xu,Xiangxiang Liu,Jian Qin,Junjie Nie,Xiaodan Qin,Shukui Wang,Yuqin Pan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LACTB antibody [EPR8502]

Western blot - Anti-LACTB antibody [EPR8502] (AB131171)

OI-RD Scanning - Anti-LACTB antibody [EPR8502] (AB131171)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise