Rabbit Polyclonal LACTB2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LACTB2 aa 1 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human LACTB2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q53H82
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Target data
Function
Endoribonuclease; cleaves preferentially 3' to purine-pyrimidine dinucleotide motifs in single-stranded RNA. The cleavage product contains a free 3' -OH group. Has no activity with double-stranded RNA or DNA. Required for normal mitochondrial function and cell viability.
Alternative names
CGI-83, LACTB2, Endoribonuclease LACTB2, Beta-lactamase-like protein 2
Recommended products
Rabbit Polyclonal LACTB2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LACTB2 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human LACTB2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q53H82
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LACTB2 also known as lactamase beta 2 functions primarily as a mitochondrial serine protease. This protein typically exhibits a mass of around 40 kDa. LACTB2 shows expression largely within mitochondrial tissue indicating its specialized function in this cellular environment. The presence in mitochondria connects LACTB2 to cellular processes involving the regulation of protein composition within these organelles.
- Biological function summary
The activity of LACTB2 influences mitochondrial morphology and function. Within this context LACTB2 acts as a solitary protein and is not identified as part of a larger protein complex. Through its protease activity LACTB2 participates in the processing of mitochondrial proteins reflecting its role in maintaining protein homeostasis in the mitochondria.
- Pathways
Mitochondrial dynamics and oxidative phosphorylation rely on the function of proteins like LACTB2. LACTB2 plays a part in pathways managing mitochondrial quality control including interactions with proteins such as OPA1 and MFN2 that help modulate mitochondrial fusion and fission. These interactions emphasize the vital role LACTB2 has in sustaining mitochondrial integrity and function impacting energy production and cellular stress responses.
- Associated diseases and disorders
LACTB2 has associations with various mitochondrial disorders and neurodegenerative diseases such as Alzheimer's disease. The function of LACTB2 affects the stability and function of proteins like amyloid precursor protein (APP) which is linked to Alzheimer's pathology. Understanding these relationships highlights the importance of LACTB2 in neurological health and disease where dysfunctions in mitochondrial processes occur frequently.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-LACTB2 antibody (ab154267)
12% SDS PAGE
All lanes: Western blot - Anti-LACTB2 antibody (ab154267) at 1/1000 dilution
All lanes: A549 whole cell lysate at 30 µg
Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB2 antibody (ab154267)
Immunohistochemical analysis of paraffin embedded Human colon ca tissue, labeling LACTB2 with ab154267 at 1/500 dilution.
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