AB154267

Anti-LACTB2 antibody

Name: Anti-LACTB2 antibody (ab154267) | Abcam
Brand: Abcam Limited
SKU: AB154267

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(1 Publication)

Rabbit Polyclonal LACTB2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LACTB2 aa 1 to C-terminus.

View Alternative Names

CGI-83, LACTB2, Endoribonuclease LACTB2, Beta-lactamase-like protein 2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB2 antibody (AB154267)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB2 antibody (AB154267)

Immunohistochemical analysis of paraffin embedded Human colon ca tissue, labeling LACTB2 with ab154267 at 1/500 dilution.

Unknown

Western blot - Anti-LACTB2 antibody (AB154267)

12% SDS PAGE

All lanes:

Western blot - Anti-LACTB2 antibody (ab154267) at 1/1000 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 32 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human LACTB2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q53H82

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LACTB2 also known as lactamase beta 2 functions primarily as a mitochondrial serine protease. This protein typically exhibits a mass of around 40 kDa. LACTB2 shows expression largely within mitochondrial tissue indicating its specialized function in this cellular environment. The presence in mitochondria connects LACTB2 to cellular processes involving the regulation of protein composition within these organelles.

Biological function summary

The activity of LACTB2 influences mitochondrial morphology and function. Within this context LACTB2 acts as a solitary protein and is not identified as part of a larger protein complex. Through its protease activity LACTB2 participates in the processing of mitochondrial proteins reflecting its role in maintaining protein homeostasis in the mitochondria.

Pathways

Mitochondrial dynamics and oxidative phosphorylation rely on the function of proteins like LACTB2. LACTB2 plays a part in pathways managing mitochondrial quality control including interactions with proteins such as OPA1 and MFN2 that help modulate mitochondrial fusion and fission. These interactions emphasize the vital role LACTB2 has in sustaining mitochondrial integrity and function impacting energy production and cellular stress responses.

LACTB2 has associations with various mitochondrial disorders and neurodegenerative diseases such as Alzheimer's disease. The function of LACTB2 affects the stability and function of proteins like amyloid precursor protein (APP) which is linked to Alzheimer's pathology. Understanding these relationships highlights the importance of LACTB2 in neurological health and disease where dysfunctions in mitochondrial processes occur frequently.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endoribonuclease; cleaves preferentially 3' to purine-pyrimidine dinucleotide motifs in single-stranded RNA. The cleavage product contains a free 3' -OH group. Has no activity with double-stranded RNA or DNA. Required for normal mitochondrial function and cell viability.

See full target information LACTB2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eadl4374 PubMed39383215

2024

Loss of atrial natriuretic peptide signaling causes insulin resistance, mitochondrial dysfunction, and low endurance capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Deborah Carper,Marlène Lac,Marine Coue,Axel Labour,Andre Märtens,Jorge Alberto Ayala Banda,Laurène Mazeyrie,Mie Mechta,Lars Roed Ingerslev,Mohamed Elhadad,Justine Vily Petit,Claire Maslo,Laurent Monbrun,Peggy Del Carmine,Yannis Sainte-Marie,Virginie Bourlier,Claire Laurens,Gilles Mithieux,Denis R Joanisse,Charles Coudray,Christine Feillet-Coudray,Emilie Montastier,Nathalie Viguerie,Geneviève Tavernier,Melanie Waldenberger,Annette Peters,Rui Wang-Sattler,Jerzy Adamski,Karsten Suhre,Christian Gieger,Gabi Kastenmüller,Thomas Illig,Ralf Lichtinghagen,Jochen Seissler,Remy Mounier,Karsten Hiller,Jens Jordan,Romain Barrès,Michaela Kuhn,Dominik Pesta,Cedric Moro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LACTB2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LACTB2 antibody (AB154267)

Western blot - Anti-LACTB2 antibody (AB154267)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise