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AB246885

Anti-LAD1 antibody

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(3 Publications)

Rabbit Polyclonal LAD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LAD1 aa 350 to C-terminus.

View Alternative Names

LAD, LAD1, Ladinin-1, Lad-1, Linear IgA disease antigen, LADA

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAD1 antibody (AB246885)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAD1 antibody (AB246885)

Paraffin-embedded human skin tissue stained for LAD1 using ab246885 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-LAD1 antibody (AB246885)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LAD1 antibody (AB246885)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for LAD1 (green) using ab246885 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAD1 antibody (AB246885)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAD1 antibody (AB246885)

Paraffin-embedded human liver tissue stained for LAD1 using ab246885 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAD1 antibody (AB246885)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAD1 antibody (AB246885)

Paraffin-embedded human kidney tissue stained for LAD1 using ab246885 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAD1 antibody (AB246885)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAD1 antibody (AB246885)

Paraffin-embedded human small intestine tissue stained for LAD1 using ab246885 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-LAD1 antibody (AB246885)
  • WB

Supplier Data

Western blot - Anti-LAD1 antibody (AB246885)

Loading control : Anti-PFN1.

All lanes:

Western blot - Anti-LAD1 antibody (ab246885) at 0.4 µg/mL

Lane 1:

A431 (human epidermoid carcinoma cell line) cell lysate

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human LAD1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00515

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAD1 also known as Ladinin-1 is a 74 kDa protein prominently involved in maintaining the structural integrity of the basement membrane zone in epithelial tissues. LAD1 achieves this function by interacting with other structural proteins and contributing to the stability and adhesion of epithelial cells. This protein is expressed mainly in the skin but can also be detected in other epithelial-rich organs. While playing an important role LAD1 does not act alone; rather it works synergistically with cytoskeletal and extracellular components.
Biological function summary

LAD1 contributes to cell adhesion ensuring strong attachment of epithelial cells to the underlying basement membrane. It forms a part of complex networks within the basement membrane zone working together with proteins like collagen and laminin. This interaction is important for cellular architecture and tissue integrity providing essential support for cells in environments subject to constant mechanical stress.

Pathways

LAD1 participates in the pathways that manage cell adhesion and integrity. It is closely associated with the integrin complex pathway which is vital for linking the extracellular matrix to the cell. LAD1 interacts with integrins and other adhesion molecules such as collagen enhancing cellular communication and structural stability. These interactions support tissue integrity enabling cells to resist shearing forces effectively.

Defects or alterations in LAD1 expression or function are related to skin conditions like epidermolysis bullosa. This disorder involves skin fragility due to improper adhesion between dermal and epidermal layers. LAD1's interactions with connective proteins like collagen XVII become disrupted further exacerbating these conditions. Abnormal LAD1 expression can contribute to the development and severity of such disorders highlighting its role in maintaining healthy skin structure.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Anchoring filament protein which is a component of the basement membrane zone.
See full target information LAD1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36613882

2022

Ladinin 1 Shortens Survival via Promoting Proliferation and Enhancing Invasiveness in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chao-Yuan Chang,Yung-Chi Huang,Hung-Hsing Chiang,Yu-Yuan Wu,Kuan-Li Wu,Yung-Yun Chang,Lian-Xiu Liu,Ying-Ming Tsai,Ya-Ling Hsu

Journal of cellular and molecular medicine 25:3803-3815 PubMed33621420

2021

Isoflurane post-conditioning contributes to anti-apoptotic effect after cerebral ischaemia in rats through the ERK5/MEF2D signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qingtong Zhang,Jiangwen Yin,Feng Xu,Jingwen Zhai,Jieting Yin,Mingyue Ge,Wenyi Zhou,Nian Li,Xinlei Qin,Yan Li,Sheng Wang

Cancer cell international 21:85 PubMed33536022

2021

circ-ANXA7 facilitates lung adenocarcinoma progression via miR-331/LAD1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang
View all publications
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Product promise

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