Rabbit Polyclonal LAE antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACSM1 aa 1-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ACSM1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q08AH1
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Target data
Function
Catalyzes the activation of fatty acids by CoA to produce an acyl-CoA, the first step in fatty acid metabolism (PubMed:10434065). Capable of activating medium-chain fatty acids (e.g. butyric (C4) to decanoic (C10) acids), and certain carboxylate-containing xenobiotics, e.g. benzoate (PubMed:10434065). Also catalyzes the activation of lipoate to lipoyl-nucleoside monophosphate (By similarity). Activates lipoate with GTP at a 1000-fold higher rate than with ATP and activates both (R)- and (S)-lipoate to the respective lipoyl-GMP, with a preference for (R)-lipoate (By similarity).
Alternative names
BUCS1, LAE, MACS1, ACSM1, Acyl-CoA synthetase medium-chain family member 1, Benzoate--CoA ligase, Butyrate--CoA ligase 1, Butyryl-coenzyme A synthetase 1, Lipoate-activating enzyme, Middle-chain acyl-CoA synthetase 1, Xenobiotic/medium-chain fatty acid-CoA ligase HXM-B
Recommended products
Rabbit Polyclonal LAE antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ACSM1 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ACSM1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q08AH1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target LAE also known by some researchers as HXLM is a notable protein with a mass typically around 58 kDa. It is located throughout various tissues but shows high expression levels in the liver and kidneys. Specifically LAE is recognized for its binding affinity and interaction with several cellular substrates playing a direct role in various cellular activities. The protein's structural characteristics allow it to engage in essential biochemical reactions within the cell.
- Biological function summary
LAE contributes significantly to cellular metabolism and regulation processes. It is part of a multimeric complex where it functions collaboratively with other proteins to maintain cellular balance. LAE is involved in energy production and molecule conversion making it critical in supporting cells under stress conditions. Its complex formation aids in transporting molecules across the cell membrane linking it to essential cellular operations.
- Pathways
LAE interacts predominantly with the glycolysis and gluconeogenesis pathways. In these pathways it associates with the enzyme hexokinase assisting in glucose breakdown and synthesis. This protein's involvement ensures efficient energy circulation and utilization within the cell. Additionally LAE connects with phosphofructokinase in modulating pathway activities highlighting its influence on broader metabolic functions.
- Associated diseases and disorders
LAE has strong connections to diabetes and renal dysfunction. Dysregulation of this protein can result in abnormal glucose metabolism a hallmark of diabetic conditions. When linked to disorders LAE's relationship with insulin-like growth factor binding protein plays a part in managing glucose levels and kidney health. Such associations deepen the understanding of LAE's potential as a therapeutic target in treating metabolic and renal diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-LAE antibody (ab151456)
7.5% SDS PAGE
All lanes: Western blot - Anti-LAE antibody (ab151456) at 1/1000 dilution
Lane 1: Jurkat whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Lane 3: THP1 whole cell lysate at 30 µg
Predicted band size: 65 kDa, 88 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAE antibody (ab151456)
Immunohistochemical analysis of paraffin embedded U87 xenograft tissue labeling LAE with ab151456 at 1/500.
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