Mouse Monoclonal LAG-3 antibody. Suitable for IP, BL, Flow Cyt, ELISA, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Monkey, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LAG3.
Images
![Flow Cytometry - Anti-LAG-3 antibody [11E3] (AB40465), expandable thumbnail](https://content.abcam.com/products/images/lag-3-antibody-11e3-ab40465--flow-cytometry-img114060.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-LAG-3 antibody [11E3] (AB40465)")
Publications
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- OncoTargets and therapy 13:9785-97952020MiRNA505/NET1 Axis Acts as a CD8 T-TIL Regulator in Non-Small Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengyuan Zhu et. al.PubMed 33061457
- Leukemia research reports 14:1002152020Immune checkpoints bone marrow expression as the predictor of clinical outcome in myelodysplastic syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNikolai Tcvetkov et. al.PubMed 32695574
- Journal of clinical laboratory analysis 34:e232442020Ectopic expression of LAG-3 in non-small-cell lung cancer cells and its clinical significance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenglong Ma et. al.PubMed 32077528
- Annals of translational medicine 7:7762020Characterization of LAG-3, CTLA-4, and CD8 TIL density and their joint influence on the prognosis of patients with esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjia Wang,Donglai Chen,Yuhuan Zhao,Ting Zhao,Junmiao Wen,Yiming Mao,Chang Chen,Yonghua Sang,Yongsheng Zhang,Yongbing ChenPubMed 32042792
- Journal of immunotherapy (Hagerstown, Md. : 1997) 42:279-2832019LAG3 in Solid Tumors as a Potential Novel Immunotherapy Target.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSu Jin Lee,Sun-Ju Byeon,Jeeyun Lee,Se Hoon Park,Joon Oh Park,Young Suk Park,Won Ki Kang,Ho Yeong Lim,Kyoung-Mee Kim,Seung Tae KimPubMed 31219974
- Journal of the American Academy of Dermatology 81:219-2272019Expression of lymphocyte-activating gene 3 and T-cell immunoreceptor with immunoglobulin and ITIM domains in cutaneous melanoma and their correlation with programmed cell death 1 expression in tumor-infiltrating lymphocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWoo Jin Lee et. al.PubMed 30880064
- Journal of translational medicine 16:862018Use of the tumor-infiltrating CD8 to FOXP3 lymphocyte ratio in predicting treatment responses to combination therapy with pertuzumab, trastuzumab, and docetaxel for advanced HER2-positive breast cancer.
- Proceedings of the National Academy of Sciences of the United States of America 94:5744-91997Characterization of the major histocompatibility complex class II binding site on LAG-3 protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesB Huard,R Mastrangeli,P Prigent,D Bruniquel,S Donini,N El-Tayar,B Maigret,M Dréano,F TriebelPubMed 9159144
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human LAG3. Database link P18627
Abcam Recommends
Reactivity data
Select an application| IP | BL | Flow Cyt | ELISA | WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|---|---|---|
| Human | Expected | Expected | Tested | Expected | Expected | Expected | Expected | Expected |
| Monkey | Expected | Expected | Predicted | Expected | Expected | Expected | Expected | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
BL
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:20421648, PubMed:7805750, PubMed:8647185). Delivers inhibitory signals upon binding to ligands, such as FGL1 (By similarity). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (By similarity). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (By similarity). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:20421648, PubMed:7805750, PubMed:8647185). Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (By similarity). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (By similarity). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:8647185). Secreted lymphocyte activation gene 3 protein. May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.
Alternative names
CD223, FDC, LAG3, Lymphocyte activation gene 3 protein, LAG-3
Recommended products
Mouse Monoclonal LAG-3 antibody. Suitable for IP, BL, Flow Cyt, ELISA, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Monkey, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human LAG3.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human LAG3. Database link P18627
- Clone number
- 11E3
- Purification technique
- Affinity purification Protein A
- Specificity
Recognizes the first N-terminal D1 domain of LAG-3.
- Concentration
- Purification notes
Purified from concentrated hybridoma tissue culture supernatant by Protein A Sepharose CL-4B Flow Fast Column. Purity >95% (SDS-PAGE).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LAG-3 also known as CD223 is a transmembrane protein with a mass of approximately 70 kDa. It acts as a checkpoint molecule similar to CTLA-4 and PD-1 in the immune system. LAG-3 is expressed on activated T cells natural killer cells and some dendritic cells. It interacts with its ligand MHC class II leading to inhibition of cellular proliferation and cytokine secretion an important part of immune response regulation.
- Biological function summary
LAG-3 is involved in maintaining immune homeostasis and preventing autoimmunity. It is not part of a complex but works in synergy with other immune checkpoint molecules. When interacting with MHC class II LAG-3 transmits inhibitory signals that attenuate immune cell responses. Its role in immune regulation helps to balance immune activation and tolerance which is essential for preventing tissue damage while protecting against pathogens.
- Pathways
The inhibitory role of LAG-3 integrates into the T cell receptor (TCR) signaling pathway and is also linked to the PD-1 signaling pathway. In TCR signaling LAG-3 negatively regulates T cell activation. LAG-3 is functionally related to proteins like PD-1 and CTLA-4 sharing similar roles in immune checkpoint pathways that are critical for maintaining immune balance.
- Associated diseases and disorders
LAG-3 has links to cancer and autoimmune diseases. In cancer LAG-3 expression on tumor-infiltrating lymphocytes correlates with immune evasion by tumors. This relationship appears in various cancers including melanoma and renal cell carcinoma. In autoimmune diseases LAG-3's expression on regulatory T cells helps control conditions such as multiple sclerosis. It collaborates with CTLA-4 in modulating immune responses that prevent excessive immune activation and tissue damage associated with autoimmunity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - Anti-LAG-3 antibody [11E3] (ab40465), expandable thumbnail](https://content.abcam.com/products/images/lag-3-antibody-11e3-ab40465--flow-cytometry-img114060.jpg "Flow Cytometry - Anti-LAG-3 antibody [11E3] (ab40465)")
Flow Cytometry - Anti-LAG-3 antibody [11E3] (ab40465)
Overlay histogram showing Jurkat cells stained with ab40465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40465, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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