Skip to main content

Rabbit Recombinant Monoclonal LAG-3 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human LAG3 aa 500 to C-terminus.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-LAG-3 antibody [BLR027F] (AB243884), expandable thumbnail
  • Immunocytochemistry - Anti-LAG-3 antibody [BLR027F] (AB243884), expandable thumbnail
  • Western blot - Anti-LAG-3 antibody [BLR027F] (AB243884), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] (AB243884), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] (AB243884), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human LAG3 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P18627

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICCIPWB
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/500.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/500.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Use 12μl/0.5mg lysate.

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:20421648, PubMed:7805750, PubMed:8647185). Delivers inhibitory signals upon binding to ligands, such as FGL1 (By similarity). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (By similarity). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (By similarity). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:20421648, PubMed:7805750, PubMed:8647185). Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (By similarity). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (By similarity). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:8647185). Secreted lymphocyte activation gene 3 protein. May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.

Alternative names

CD223, FDC, LAG3, Lymphocyte activation gene 3 protein, LAG-3

Recommended products

Rabbit Recombinant Monoclonal LAG-3 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human LAG3 aa 500 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human LAG3 aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P18627
Clone number
BLR027F
Concentration
Loading...
Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is sold under License from Bethyl Laboratories, Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAG-3 also known as CD223 is a transmembrane protein with a mass of approximately 70 kDa. It acts as a checkpoint molecule similar to CTLA-4 and PD-1 in the immune system. LAG-3 is expressed on activated T cells natural killer cells and some dendritic cells. It interacts with its ligand MHC class II leading to inhibition of cellular proliferation and cytokine secretion an important part of immune response regulation.

Biological function summary

LAG-3 is involved in maintaining immune homeostasis and preventing autoimmunity. It is not part of a complex but works in synergy with other immune checkpoint molecules. When interacting with MHC class II LAG-3 transmits inhibitory signals that attenuate immune cell responses. Its role in immune regulation helps to balance immune activation and tolerance which is essential for preventing tissue damage while protecting against pathogens.

Pathways

The inhibitory role of LAG-3 integrates into the T cell receptor (TCR) signaling pathway and is also linked to the PD-1 signaling pathway. In TCR signaling LAG-3 negatively regulates T cell activation. LAG-3 is functionally related to proteins like PD-1 and CTLA-4 sharing similar roles in immune checkpoint pathways that are critical for maintaining immune balance.

Associated diseases and disorders

LAG-3 has links to cancer and autoimmune diseases. In cancer LAG-3 expression on tumor-infiltrating lymphocytes correlates with immune evasion by tumors. This relationship appears in various cancers including melanoma and renal cell carcinoma. In autoimmune diseases LAG-3's expression on regulatory T cells helps control conditions such as multiple sclerosis. It collaborates with CTLA-4 in modulating immune responses that prevent excessive immune activation and tissue damage associated with autoimmunity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-LAG-3 antibody [BLR027F] (ab243884), expandable thumbnail

    Immunoprecipitation - Anti-LAG-3 antibody [BLR027F] (ab243884)

    LAG-3 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243884 at 12 μl per reaction. Western blot was performed on the immunoprecipitate using ab243884 at 1/1000 dilution.

    Lane 1: ab243884 IP in HDLM-2 whole cell lysate.

    Lane 2: Control IgG IP in HDLM-2 whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

    All lanes: Immunoprecipitation - Anti-LAG-3 antibody [BLR027F] (ab243884)

    Predicted band size: 57 kDa

  • Immunocytochemistry - Anti-LAG-3 antibody [BLR027F] (ab243884), expandable thumbnail

    Immunocytochemistry - Anti-LAG-3 antibody [BLR027F] (ab243884)

    Formalin-fixed, paraffin-embedded HDLM-2 cells labeling LAG-3 using ab243884 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

  • Western blot - Anti-LAG-3 antibody [BLR027F] (ab243884), expandable thumbnail

    Western blot - Anti-LAG-3 antibody [BLR027F] (ab243884)

    Western blot analysis using ab243884 at 1/1000 dilution.

    Lane 1: HDLM-2 whole cell lysate (50 µg).

    Lane 2: RPMI 8226 whole cell lysate (50 µg).

    Lane3: Jurkat whole cell lysate (50 µg).

    Lane 4: MJ whole cell lysate (50 µg).

    Lane 5: HEK-293T whole cell lysate (50 µg).

    A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.

    All lanes: Western blot - Anti-LAG-3 antibody [BLR027F] (ab243884)

    Predicted band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] (ab243884), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] (ab243884)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab243884 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] (ab243884), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] (ab243884)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 (red) using ab243884 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. Counterstain: DAPI (blue).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com