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Mouse Recombinant Monoclonal LAG-3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB307518), expandable thumbnail
  • Immunohistochemistry - Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB307518), expandable thumbnail
  • Immunohistochemistry - Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) - BSA and Azide free (AB307518), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBFlow CytICC/IFIP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:20421648, PubMed:7805750, PubMed:8647185). Delivers inhibitory signals upon binding to ligands, such as FGL1 (By similarity). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (By similarity). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (By similarity). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (By similarity). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:20421648, PubMed:7805750, PubMed:8647185). Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (By similarity). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (By similarity). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:8647185). Secreted lymphocyte activation gene 3 protein. May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.

Alternative names

CD223, FDC, LAG3, Lymphocyte activation gene 3 protein, LAG-3

Recommended products

Mouse Recombinant Monoclonal LAG-3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR20261
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

This mouse monoclonal chimeric antibody (ab307518) is the carrier-free version of Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) ab307517

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAG-3 also known as CD223 is a transmembrane protein with a mass of approximately 70 kDa. It acts as a checkpoint molecule similar to CTLA-4 and PD-1 in the immune system. LAG-3 is expressed on activated T cells natural killer cells and some dendritic cells. It interacts with its ligand MHC class II leading to inhibition of cellular proliferation and cytokine secretion an important part of immune response regulation.

Biological function summary

LAG-3 is involved in maintaining immune homeostasis and preventing autoimmunity. It is not part of a complex but works in synergy with other immune checkpoint molecules. When interacting with MHC class II LAG-3 transmits inhibitory signals that attenuate immune cell responses. Its role in immune regulation helps to balance immune activation and tolerance which is essential for preventing tissue damage while protecting against pathogens.

Pathways

The inhibitory role of LAG-3 integrates into the T cell receptor (TCR) signaling pathway and is also linked to the PD-1 signaling pathway. In TCR signaling LAG-3 negatively regulates T cell activation. LAG-3 is functionally related to proteins like PD-1 and CTLA-4 sharing similar roles in immune checkpoint pathways that are critical for maintaining immune balance.

Associated diseases and disorders

LAG-3 has links to cancer and autoimmune diseases. In cancer LAG-3 expression on tumor-infiltrating lymphocytes correlates with immune evasion by tumors. This relationship appears in various cancers including melanoma and renal cell carcinoma. In autoimmune diseases LAG-3's expression on regulatory T cells helps control conditions such as multiple sclerosis. It collaborates with CTLA-4 in modulating immune responses that prevent excessive immune activation and tissue damage associated with autoimmunity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab307518), expandable thumbnail

    Western blot - Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab307518)

    This data was developed using Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) ab307517, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Jurkat (PMID: 25108024).

    The expression profile observed in Jurkat is consistent with the literature (PMID: 25108024).

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) (Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) ab307517) at 1/1000 dilution

    Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate 20 μg

    Lane 2: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate 20 μg

    Observed band size: 54 kDa, 70 kDa

    Exposure time: 26s

  • Immunohistochemistry - Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab307518), expandable thumbnail

    Immunohistochemistry - Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab307518)

    This data was developed using Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) ab307517, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LAG-3 with Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) ab307517 at 1/1000 dilution (1.068 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on human tonsil.
    The section was incubated with Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) ab307517 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry - Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab307518), expandable thumbnail

    Immunohistochemistry - Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab307518)

    This data was developed using Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) ab307517, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling LAG-3 with Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) ab307517 at 1/1000 dilution (1.068 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Positive staining on human Hodgkin's lymphoma.
    The section was incubated with Anti-LAG-3 antibody [EPR20261] - Mouse IgG1 (Chimeric) ab307517 for 30 mins at room temperature, followed by anti-mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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