JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB171239

Anti-LAIR1 antibody [113] - Low endotoxin, Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Armenian hamster Monoclonal LAIR1 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Lair1 protein.

View Alternative Names

CD305, Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, mLAIR1, Lair1

1 Images
Flow Cytometry - Anti-LAIR1 antibody [113] - Low endotoxin, Azide free (AB171239)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-LAIR1 antibody [113] - Low endotoxin, Azide free (AB171239)

Flow cytometry analysis of C57B1/6 splenocytes (total viable F4/80 positive cells) labelling LAIR1 with ab171239 at 0.125μg (blue line) and with Purified Armenian Hamster IgG control (purple line), followed by anti-Armenian Hamster IgG PE.

  • 578 PE

    PE Anti-LAIR1 antibody [113]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

113

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Lair1 protein. The exact immunogen used to generate this antibody is proprietary information.

Q8BG84

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>ab171239 can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.</p><p><a href='/en-us/products/primary-antibodies/armenian-hamster-igg-monoclonal-htk888-isotype-control-ab18479'>ab18479</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

Product details

Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by the LAL assay.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAIR1 also known as Leukocyte-Associated Immunoglobulin-like Receptor 1 is a transmembrane protein with a molecular weight of approximately 32 kDa. It expresses mainly on immune cells including T cells B cells and myeloid cells. This receptor by binding to collagen plays a significant role in controlling immune cell signaling. It acts as an inhibitory receptor which helps in maintaining immune system balance and prevents excessive immune responses.
Biological function summary

LAIR1 functions by modulating immune responses and maintaining homeostasis in the immune system. It is a part of a complex pathway involved in inhibitory signaling. When LAIR1 binds to its ligand such as collagen it transduces a signal that reduces activation of immune cells. This inhibition ensures that immune cells do not become overactive which could otherwise lead to tissue damage.

Pathways

LAIR1 participates in immune checkpoint regulation and collagen interaction pathways. It interacts with proteins such as ITIM-containing molecules which transmit inhibitory signals essential for regulating immune cell activation. It also indirectly connects to the TGF-beta signaling pathway which is vital for controlling cell differentiation and immune regulation.

LAIR1 is of interest due to its connection to autoimmune diseases and certain cancers. In autoimmune diseases such as rheumatoid arthritis dysregulated LAIR1 signaling may contribute to inflammation. The protein is also linked to cancer where its inhibitory signaling might affect tumor immune evasion. The connection between LAIR1 and these conditions involves proteins like autoimmune-related genes and tumor-associated surface molecules.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Also down-regulates IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells (By similarity).
See full target information Lair1

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 17:665-77 PubMed25915125

2015

The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development.

Applications

WB

Species

Unspecified reactive species

Xunlei Kang,Zhigang Lu,Changhao Cui,Mi Deng,Yuqi Fan,Baijun Dong,Xin Han,Fuchun Xie,Jeffrey W Tyner,John E Coligan,Robert H Collins,Xiangshu Xiao,M James You,Cheng Cheng Zhang
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com