AB189412

Anti-LAIR1 antibody

Name: Anti-LAIR1 antibody (ab189412) | Abcam
Brand: Abcam Limited
SKU: AB189412
Price: 630 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal LAIR1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human LAIR1 aa 50-100.

View Alternative Names

CD305, LAIR1, Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, hLAIR1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody (AB189412)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody (AB189412)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling LAIR1 with ab189412 at 3.75μg/ml.

Supplier Data

Western blot - Anti-LAIR1 antibody (AB189412)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-LAIR1 antibody (ab189412) at 2 µg/mL

All lanes:

Human Lymph Node lysate (in RIPA buffer) at 35 µg

Predicted band size: 31 kDa

Observed band size: 28 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human LAIR1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q6GTX8

Specificity

ab189412 is expected to recognize isoforms NP_002278.1 and NP_068352.1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAIR1 also known as Leukocyte-Associated Immunoglobulin-like Receptor 1 is a transmembrane protein with a molecular weight of approximately 32 kDa. It expresses mainly on immune cells including T cells B cells and myeloid cells. This receptor by binding to collagen plays a significant role in controlling immune cell signaling. It acts as an inhibitory receptor which helps in maintaining immune system balance and prevents excessive immune responses.

Biological function summary

LAIR1 functions by modulating immune responses and maintaining homeostasis in the immune system. It is a part of a complex pathway involved in inhibitory signaling. When LAIR1 binds to its ligand such as collagen it transduces a signal that reduces activation of immune cells. This inhibition ensures that immune cells do not become overactive which could otherwise lead to tissue damage.

Pathways

LAIR1 participates in immune checkpoint regulation and collagen interaction pathways. It interacts with proteins such as ITIM-containing molecules which transmit inhibitory signals essential for regulating immune cell activation. It also indirectly connects to the TGF-beta signaling pathway which is vital for controlling cell differentiation and immune regulation.

LAIR1 is of interest due to its connection to autoimmune diseases and certain cancers. In autoimmune diseases such as rheumatoid arthritis dysregulated LAIR1 signaling may contribute to inflammation. The protein is also linked to cancer where its inhibitory signaling might affect tumor immune evasion. The connection between LAIR1 and these conditions involves proteins like autoimmune-related genes and tumor-associated surface molecules.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.

See full target information LAIR1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular carcinogenesis 63:1827-1841 PubMed39016636

2024

LAIR1 promotes hepatocellular carcinoma cell metastasis and induces M2-macrophage infiltration through activating AKT-IKKβ-p65 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Banglun Pan,Shuling Shen,Jun Zhao,Zhu Zhang,Dongjie Ye,Xiaoxia Zhang,Yuxin Yao,Yue Luo,Xiaoqian Wang,Nanhong Tang

Arthritis research & therapy 25:238 PubMed38062469

2023

Efficient delivery of the lncRNA LEF1-AS1 through the antibody LAIR-1 (CD305)-modified Zn-Adenine targets articular inflammation to enhance the treatment of rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaonan Zhang,Xiaoyu He,Ming Zhang,Tianyu Wu,Xiaojie Liu,Yan Zhang,Zhuobei Xie,Saisai Liu,Tian Xia,Yuanyuan Wang,Fang Wei,Hongtao Wang,Changhao Xie

Molecular medicine reports 23: PubMed33300063

2020

WWOX promotes apoptosis and inhibits autophagy in paclitaxel‑treated ovarian carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhao,Wen Wang,Wenying Pan,Yunhai Yu,Wenqian Huang,Jiangang Gao,Ying Zhang,Shiqian Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAIR1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody (AB189412)

Western blot - Anti-LAIR1 antibody (AB189412)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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