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Rabbit Recombinant Monoclonal LAIR1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples.

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Images

Western blot - Anti-LAIR1 antibody [EPR24745-12A] (AB315967), expandable thumbnail
  • Flow Cytometry - Anti-LAIR1 antibody [EPR24745-12A] (AB315967), expandable thumbnail
  • Flow Cytometry - Anti-LAIR1 antibody [EPR24745-12A] (AB315967), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/25
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.

Alternative names

CD305, LAIR1, Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, hLAIR1

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Rabbit Recombinant Monoclonal LAIR1 antibody. Suitable for Flow Cyt, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24745-12A
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAIR1 also known as Leukocyte-Associated Immunoglobulin-like Receptor 1 is a transmembrane protein with a molecular weight of approximately 32 kDa. It expresses mainly on immune cells including T cells B cells and myeloid cells. This receptor by binding to collagen plays a significant role in controlling immune cell signaling. It acts as an inhibitory receptor which helps in maintaining immune system balance and prevents excessive immune responses.

Biological function summary

LAIR1 functions by modulating immune responses and maintaining homeostasis in the immune system. It is a part of a complex pathway involved in inhibitory signaling. When LAIR1 binds to its ligand such as collagen it transduces a signal that reduces activation of immune cells. This inhibition ensures that immune cells do not become overactive which could otherwise lead to tissue damage.

Pathways

LAIR1 participates in immune checkpoint regulation and collagen interaction pathways. It interacts with proteins such as ITIM-containing molecules which transmit inhibitory signals essential for regulating immune cell activation. It also indirectly connects to the TGF-beta signaling pathway which is vital for controlling cell differentiation and immune regulation.

Associated diseases and disorders

LAIR1 is of interest due to its connection to autoimmune diseases and certain cancers. In autoimmune diseases such as rheumatoid arthritis dysregulated LAIR1 signaling may contribute to inflammation. The protein is also linked to cancer where its inhibitory signaling might affect tumor immune evasion. The connection between LAIR1 and these conditions involves proteins like autoimmune-related genes and tumor-associated surface molecules.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-LAIR1 antibody [EPR24745-12A] (ab315967), expandable thumbnail

    Western blot - Anti-LAIR1 antibody [EPR24745-12A] (ab315967)

    Lanes 1 & 2: Merged signal (red and green). Green – ab315967 observed at 40 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.

    ab315967 was shown to specifically react with LAIR1. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. ab315967 and Anti-alpha Tubulin antibody [DM1A]- Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilutions, respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-LAIR1 antibody [EPR24745-12A] (ab315967) at 1/1000 dilution

    Lane 1: THP-1 cell lysate at 20 µg with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

    Lane 2: HeLa cell lysate with 3% milk in TBS-0.1 % Tween® 20 (TBS-T)

    Secondary

    Lanes 1 - 2: Goat anti-Rabbit IgG H&L (IRDye® 800CW) at 1/20000 dilution

    Lanes 1 - 2: Goat anti-Mouse IgG H&L (IRDye® 680RD) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 40 kDa, 50 kDa

  • Flow Cytometry - Anti-LAIR1 antibody [EPR24745-12A] (ab315967), expandable thumbnail

    Flow Cytometry - Anti-LAIR1 antibody [EPR24745-12A] (ab315967)

    Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab315967 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab315967 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 10⁶ in 100 µl at 0.2 µg/ml (1/25)) for 30min on ice. The cells were simultaneously stained with CD19.

    The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/2000 for 30min on ice.

    Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable CD3(-) lymphocytes.

  • Flow Cytometry - Anti-LAIR1 antibody [EPR24745-12A] (ab315967), expandable thumbnail

    Flow Cytometry - Anti-LAIR1 antibody [EPR24745-12A] (ab315967)

    Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab315967(right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab315967 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control(1x 10⁶ in 100 µl at 0.2 µg/ml (1/25)) for 30min on ice. The cells were simultaneously stained with CD14.

    The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/2000 for 30min on ice.

    Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable monocytes.

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Product protocols

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