Skip to main content

Rabbit Recombinant Monoclonal LAIR1 antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-LAIR1 antibody [EPR24745-9] (AB323640), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (AB323640), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (AB323640), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (AB323640), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (AB323640), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Recombinant fragment - Human
Tested
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.

Alternative names

CD305, LAIR1, Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, hLAIR1

Rabbit Recombinant Monoclonal LAIR1 antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24745-9
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-LAIR1 antibody [EPR24745-9] (ab323640), expandable thumbnail

    Western blot - Anti-LAIR1 antibody [EPR24745-9] (ab323640)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with recombinant human LAIR2 by western blot.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-LAIR1 antibody [EPR24745-9] (ab323640) at 1/1000 dilution

    Lane 1: His-tagged human LAIR1 fragment at 10 ng

    Lane 2: His-tagged human LAIR2 fragment at 10 ng

    Observed band size: 31 kDa, 23 kDa

    Exposure time: 92s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (ab323640), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (ab323640)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling LAIR1 with ab323640 at 1/100 (4.89 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: No staining in human skeletal muscle.

    The section was incubated with ab323640 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (ab323640), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (ab323640)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling LAIR1 with ab323640 at 1/100 (4.89 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human lung carcinoma.

    The section was incubated with ab323640 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (ab323640), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (ab323640)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling LAIR1 with ab323640 at 1/100 (4.89 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on immunocytes of human colon.

    The section was incubated with ab323640 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (ab323640), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (ab323640)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling LAIR1 with ab323640 at 1/100 (4.89 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human tonsil.

    The section was incubated with ab323640 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (ab323640), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAIR1 antibody [EPR24745-9] (ab323640)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LAIR1 with ab323640 at 1/100 (4.89 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on Kupffer cells of human liver (PMID: 31019980).

    The section was incubated with ab323640 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-LAIR1 antibody [EPR24745-9] (ab323640), expandable thumbnail

    Western blot - Anti-LAIR1 antibody [EPR24745-9] (ab323640)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HeLa, A-172.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-LAIR1 antibody [EPR24745-9] (ab323640) at 1/1000 dilution

    Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 2: CCRF-CEM (human peripheral blood t lymphoblast) whole cell lysate at 20 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: A-172 (human brain glioblastoma cell ) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 37 kDa, 36 kDa

    Exposure time: 15s

  • Western blot - Anti-LAIR1 antibody [EPR24745-9] (ab323640), expandable thumbnail

    Western blot - Anti-LAIR1 antibody [EPR24745-9] (ab323640)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: human skeletal muscle.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-LAIR1 antibody [EPR24745-9] (ab323640) at 1/1000 dilution

    Lane 1: Human tonsil tissue lysate at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 37 kDa, 36 kDa

    Exposure time: 70s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com