AB210954

Anti-LAMA1 antibody [CL3087]

KO Validated
What is this?

(1 Review)

(1 Publication)

Mouse Monoclonal LAMA1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human LAMA1 aa 600-700.

View Alternative Names

LAMA, LAMA1, Laminin subunit alpha-1, Laminin A chain, Laminin-1 subunit alpha, Laminin-3 subunit alpha, S-laminin subunit alpha, S-LAM alpha

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (AB210954)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (AB210954)

Immunohistochemical analysis of paraffin-embedded Human testis tissue, labeling LAMA1 using ab210954 at a 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (AB210954)

Immunohistochemical analysis of paraffin-embedded Human lymph node tissue (negative control), labeling LAMA1 using ab210954 at a 1/200 dilution.

Lab

Western blot - Anti-LAMA1 antibody [CL3087] (AB210954)

Lanes 1-3 : Merged signal (red and green). Green - ab210954 observed at 337 kDa. Red - loading control ab129002 observed at 124 kDa.

ab210954 Anti-LAMA1 antibody [CL3087] was shown to specifically react with LAMA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264991 (knockout cell lysate ab258937) was used. Wild-type and LAMA1 knockout samples were subjected to SDS-PAGE. ab210954 and Anti-Vinculin antibody [EPR8185] (ab129002) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LAMA1 antibody [CL3087] (ab210954) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

LAMA1 knockout HeLa cell lysate at 20 µg

Lane 3:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution

Predicted band size: 337 kDa

Observed band size: 337 kDa

false

Supplier Data

Western blot - Anti-LAMA1 antibody [CL3087] (AB210954)

All lanes:

Western blot - Anti-LAMA1 antibody [CL3087] (ab210954) at 1/500 dilution

Lane 1:

Recombinant protein fragment of human Laminin 111

Lane 2:

Recombinant protein fragment of human Laminin 121

Lane 3:

Recombinant protein fragment of human Laminin 221

Lane 4:

Recombinant protein fragment of human Laminin 332

Lane 5:

Recombinant protein fragment of human Laminin 411

Lane 6:

Recombinant protein fragment of human Laminin 521

Predicted band size: 337 kDa

true

Supplier Data

Western blot - Anti-LAMA1 antibody [CL3087] (AB210954)

All lanes:

Western blot - Anti-LAMA1 antibody [CL3087] (ab210954) at 1/500 dilution

All lanes:

Human testis tissue lysate

Predicted band size: 337 kDa

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3087

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human LAMA1 aa 600-700. The exact immunogen used to generate this antibody is proprietary information.

P25391

Epitope

ab210954 binds to an epitope located within the peptide sequence LVPENFQDFHSKRQI (aa 640 - 654)

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAMA1 also known as laminin subunit alpha-1 is a component of the extracellular matrix with a molecular mass of approximately 400 kDa. It is part of the laminin family a group of glycoproteins that play key roles in maintaining structural integrity and signaling in tissues. LAMA1 is widely expressed in basement membranes of many tissues particularly in the brain kidney and other organs with complex architecture. Researchers often explore its expression patterns using techniques like laminin histology and laminin microscope analysis.

Biological function summary

This protein acts as a structural scaffold and cell adhesion mediator. LAMA1 integrates into the laminin-1 trimer complex composed of alpha beta and gamma chains supporting cellular attachment differentiation and migration. This assembly aids in tissue morphogenesis and repair processes. It creates an important interface between cells and the extracellular matrix influencing cellular behavior and maintaining tissue function.

Pathways

LAMA1 interacts with cellular signaling cascades such as the PI3K/AKT pathway and the TGF-beta pathway. These pathways play significant roles in cellular growth survival and differentiation. LAMA1's interaction with other proteins like integrins and heparan sulfate proteoglycans facilitates its role in these pathways impacting diverse processes like embryonic development and tissue remodeling.

LAMA1 shows a connection to conditions such as Pierson syndrome and certain congenital muscular dystrophies. Mutations in LAMA1 can lead to basement membrane defects contributing to these diseases' pathological features. Additionally interactions with proteins like collagen IV and perlecan are significant as these complexes can further influence disease severity and progression. Understanding LAMA1's role provides insights into potential therapeutic approaches for related disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

See full target information LAMA1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 11: PubMed35159261

2022

MicroRNA-100 Reduced Fetal Bovine Muscle Satellite Cell Myogenesis and Augmented Intramuscular Lipid Deposition by Modulating IGF1R.

Applications

Unspecified application

Species

Unspecified reactive species

Bilal Ahmad Mir,Elke Albrecht,Asghar Ali,Ola Hansson,Steffen Maak

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAMA1 antibody [CL3087]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (AB210954)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (AB210954)

Western blot - Anti-LAMA1 antibody [CL3087] (AB210954)

Western blot - Anti-LAMA1 antibody [CL3087] (AB210954)

Western blot - Anti-LAMA1 antibody [CL3087] (AB210954)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise