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Mouse Monoclonal LAMA1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human LAMA1 aa 600-700.


Images

Western blot - Anti-LAMA1 antibody [CL3087] (AB210954), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (AB210954), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (AB210954), expandable thumbnail
  • Western blot - Anti-LAMA1 antibody [CL3087] (AB210954), expandable thumbnail
  • Western blot - Anti-LAMA1 antibody [CL3087] (AB210954), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human LAMA1 aa 600-700. The exact immunogen used to generate this antibody is proprietary information. Database link P25391

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Rat
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/500.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/1000.00000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Target data

Function

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Alternative names

LAMA, LAMA1, Laminin subunit alpha-1, Laminin A chain, Laminin-1 subunit alpha, Laminin-3 subunit alpha, S-laminin subunit alpha, S-LAM alpha

Recommended products

Mouse Monoclonal LAMA1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human LAMA1 aa 600-700.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human LAMA1 aa 600-700. The exact immunogen used to generate this antibody is proprietary information. Database link P25391
Clone number
CL3087
Purification technique
Affinity purification Protein A
Epitope
ab210954 binds to an epitope located within the peptide sequence LVPENFQDFHSKRQI (aa 640 - 654)
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAMA1 also known as laminin subunit alpha-1 is a component of the extracellular matrix with a molecular mass of approximately 400 kDa. It is part of the laminin family a group of glycoproteins that play key roles in maintaining structural integrity and signaling in tissues. LAMA1 is widely expressed in basement membranes of many tissues particularly in the brain kidney and other organs with complex architecture. Researchers often explore its expression patterns using techniques like laminin histology and laminin microscope analysis.

Biological function summary

This protein acts as a structural scaffold and cell adhesion mediator. LAMA1 integrates into the laminin-1 trimer complex composed of alpha beta and gamma chains supporting cellular attachment differentiation and migration. This assembly aids in tissue morphogenesis and repair processes. It creates an important interface between cells and the extracellular matrix influencing cellular behavior and maintaining tissue function.

Pathways

LAMA1 interacts with cellular signaling cascades such as the PI3K/AKT pathway and the TGF-beta pathway. These pathways play significant roles in cellular growth survival and differentiation. LAMA1's interaction with other proteins like integrins and heparan sulfate proteoglycans facilitates its role in these pathways impacting diverse processes like embryonic development and tissue remodeling.

Associated diseases and disorders

LAMA1 shows a connection to conditions such as Pierson syndrome and certain congenital muscular dystrophies. Mutations in LAMA1 can lead to basement membrane defects contributing to these diseases' pathological features. Additionally interactions with proteins like collagen IV and perlecan are significant as these complexes can further influence disease severity and progression. Understanding LAMA1's role provides insights into potential therapeutic approaches for related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-LAMA1 antibody [CL3087] (ab210954), expandable thumbnail

    Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)

    Lanes 1-3: Merged signal (red and green). Green - ab210954 observed at 337 kDa. Red - loading control Anti-Vinculin antibody [EPR8185] ab129002 observed at 124 kDa.

    ab210954 Anti-LAMA1 antibody [CL3087] was shown to specifically react with LAMA1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264991 (knockout cell lysate ab258937) was used. Wild-type and LAMA1 knockout samples were subjected to SDS-PAGE. ab210954 and Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-LAMA1 antibody [CL3087] (ab210954) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: LAMA1 knockout HeLa cell lysate at 20 µg

    Lane 3: Human testis tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/10000 dilution

    Predicted band size: 337 kDa

    Observed band size: 337 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue, labeling LAMA1 using ab210954 at a 1/200 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [CL3087] (ab210954)

    Immunohistochemical analysis of paraffin-embedded Human lymph node tissue (negative control), labeling LAMA1 using ab210954 at a 1/200 dilution.

  • Western blot - Anti-LAMA1 antibody [CL3087] (ab210954), expandable thumbnail

    Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)

    All lanes: Western blot - Anti-LAMA1 antibody [CL3087] (ab210954) at 1/500 dilution

    Lane 1: Recombinant protein fragment of human Laminin 111

    Lane 2: Recombinant protein fragment of human Laminin 121

    Lane 3: Recombinant protein fragment of human Laminin 221

    Lane 4: Recombinant protein fragment of human Laminin 332

    Lane 5: Recombinant protein fragment of human Laminin 411

    Lane 6: Recombinant protein fragment of human Laminin 521

    Developed using the ECL technique.

    Predicted band size: 337 kDa

  • Western blot - Anti-LAMA1 antibody [CL3087] (ab210954), expandable thumbnail

    Western blot - Anti-LAMA1 antibody [CL3087] (ab210954)

    All lanes: Western blot - Anti-LAMA1 antibody [CL3087] (ab210954) at 1/500 dilution

    All lanes: Human testis tissue lysate

    Developed using the ECL technique.

    Predicted band size: 337 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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