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AB289814

Anti-LAMA1 antibody [EPR24650-123] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal LAMA1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

LAMA, LAMA1, Laminin subunit alpha-1, Laminin A chain, Laminin-1 subunit alpha, Laminin-3 subunit alpha, S-laminin subunit alpha, S-LAM alpha

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Sandwich ELISA - Anti-LAMA1 antibody [EPR24650-123] - BSA and Azide free (Capture) (AB289814)
  • sELISA

Lab

Sandwich ELISA - Anti-LAMA1 antibody [EPR24650-123] - BSA and Azide free (Capture) (AB289814)

Sandwich ELISA of ab289802 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24650-123

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Recognises laminin subunit alpha-1.

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Reactivity data

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Product details

Recognises laminin subunit alpha-1.

ab289814 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human LAMA1. The recommended pair for sandwich ELISA is:
Capture: ab289814, Human LAMA Capture Antibody (unconjugated)
Detector: ab289815, Human LAMA Detector Antibody (unconjugated)
The reference range value is 7.8-2000 pg/mL.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAMA1 also known as laminin subunit alpha-1 is a component of the extracellular matrix with a molecular mass of approximately 400 kDa. It is part of the laminin family a group of glycoproteins that play key roles in maintaining structural integrity and signaling in tissues. LAMA1 is widely expressed in basement membranes of many tissues particularly in the brain kidney and other organs with complex architecture. Researchers often explore its expression patterns using techniques like laminin histology and laminin microscope analysis.
Biological function summary

This protein acts as a structural scaffold and cell adhesion mediator. LAMA1 integrates into the laminin-1 trimer complex composed of alpha beta and gamma chains supporting cellular attachment differentiation and migration. This assembly aids in tissue morphogenesis and repair processes. It creates an important interface between cells and the extracellular matrix influencing cellular behavior and maintaining tissue function.

Pathways

LAMA1 interacts with cellular signaling cascades such as the PI3K/AKT pathway and the TGF-beta pathway. These pathways play significant roles in cellular growth survival and differentiation. LAMA1's interaction with other proteins like integrins and heparan sulfate proteoglycans facilitates its role in these pathways impacting diverse processes like embryonic development and tissue remodeling.

LAMA1 shows a connection to conditions such as Pierson syndrome and certain congenital muscular dystrophies. Mutations in LAMA1 can lead to basement membrane defects contributing to these diseases' pathological features. Additionally interactions with proteins like collagen IV and perlecan are significant as these complexes can further influence disease severity and progression. Understanding LAMA1's role provides insights into potential therapeutic approaches for related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
See full target information LAMA1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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