Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

This data was developed using ab315275, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling LAMA1 with ab315275 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on basement membrane of human kidney. The section was incubated with ab315275 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

This data was developed using ab315275, the same antibody clone in a different buffer formulation.

Panel A : anti-Oligodendrocyte Specific Protein (green; Opal™520), anti-LAMA1 (magenta; Opal™690) and anti-DDX/MVH (gray; Opal™570) on human testis. Panel B : anti-Oligodendrocyte Specific Protein staining apical Sertoli cell crypts in human testis. Panel C : anti-LAMA1 staining basement membrane in human testis. Panel D : anti-DDX4/MVH staining spermatogonia in human testis. Nuclear DNA was labeled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab323317, ab315275 and ab270534 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

This data was developed using ab315275, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling LAMA1 with ab315275 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on basement membrane of human testis. The section was incubated with ab315275 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

This data was developed using ab315275, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human testis tissue staining CYP51A1/CYP51 with ab315800 at a 1/2000 dilution, ab270534 anti-DDX4 / MVH used at 1/2000 dilution and ab315275 anti-LAMA1 used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-CYP51A1/CYP51 (green; Opal^™520), anti-DDX4 / MVH (magenta; Opal^™690) and anti-LAMA1 (grey; Opal^™570) on human testis. Panel B : anti-CYP51A1/CYP51 staining leydig cells in human testis.
Panel C : anti-DDX4 / MVH staining spermatogonia and spermatocytes in human testis.
Panel D : anti-LAMA1 staining basement membrane in human testis.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab315800, ab270834 and ab315275 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^®RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

This data was developed using ab315275, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse testis tissue staining CYP51A1/CYP51 with ab315800 at a 1/2000 dilution, ab270534 anti-DDX4 / MVH used at 1/2000 dilution and ab315275 anti-LAMA1 used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-CYP51A1/CYP51 (green; Opal^™520), anti-DDX4 / MVH (magenta; Opal^™690) and anti-LAMA1 (grey; Opal^™570) on mouse testis. Panel B : anti-CYP51A1/CYP51 staining leydig cells in mouse testis.
Panel C : anti-DDX4 / MVH staining spermatogonia and spermatocytes in mouse testis.
Panel D : anti-LAMA1 staining basement membrane in mouse testis.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab315800, ab270834 and ab315275 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^®RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

This data was developed using ab315275, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling LAMA1 with ab315275 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on basement membrane of rat testis. The section was incubated with ab315275 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

This data was developed using ab315275, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling LAMA1 with ab315275 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on basement membrane of mouse testis. The section was incubated with ab315275 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

This data was developed using ab315275, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling LAMA1 with ab315275 at 1/500 (1.018 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on basement membrane of rat kidney. The section was incubated with ab315275 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

This data was developed using ab315275, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat testis tissue staining CYP51A1/CYP51 with ab315800 at a 1/2000 dilution, ab270534 anti-DDX4 / MVH used at 1/2000 dilution and ab315275 anti-LAMA1 used at a 1/500 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

Panel A : merged staining of anti-CYP51A1/CYP51 (green; Opal^™520), anti-DDX4 / MVH (magenta; Opal^™690) and anti-LAMA1 (grey; Opal^™570) on rat testis. Panel B : anti-CYP51A1/CYP51 staining leydig cells in rat testis.
Panel C : anti-DDX4 / MVH staining spermatogonia and spermatocytes in rat testis.
Panel D : anti-LAMA1 staining basement membrane in rat testis.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab315800, ab270834 and ab315275 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^®RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

This data was developed using ab315275, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HDLM-2

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-LAMA1 antibody [EPR27259-172] (<a href='/en-us/products/primary-antibodies/lama1-antibody-epr27259-172-ab315275'>ab315275</a>) at 1/1000 dilution

Lane 1:

SK-MEL-2 (human skin malignant melanoma cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 337 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

This data was developed using ab315275, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : A431

In Western blot, ab315275 was shown to bind specifically to LAMA1. A band was observed at 337 kDa in wild-type HeLa cell lysates whereas no signal observed at this size in LAMA1 knockout cell line ab264991 (KO cell lysate ab258937).

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-LAMA1 antibody [EPR27259-172] (<a href='/en-us/products/primary-antibodies/lama1-antibody-epr27259-172-ab315275'>ab315275</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

LAMA1 knockout HeLa whole cell lysate at 20 µg

Lane 3:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 337 kDa,124 kDa

false

Exposure time: 81s

• Dot

Supplier Data

Dot Blot - Anti-LAMA1 antibody [EPR27259-172] - BSA and Azide free (AB315276)

This data was developed using ab315275, the same antibody clone in a different buffer formulation.

Dot blot analysis of LAMA1 using ab315275 at 1 : 1000 (0.509 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Exposure time : 180s.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.