AB242197

Anti-LAMA3 antibody [CL3112]

Name: Anti-LAMA3 antibody [CL3112] (ab242197) | Abcam
Brand: Abcam Limited
SKU: AB242197

(1 Review)

(7 Publications)

Mouse Monoclonal LAMA3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LAMA3 aa 2000-2150.

View Alternative Names

LAMNA, LAMA3, Laminin subunit alpha-3, Epiligrin 170 kDa subunit, Epiligrin subunit alpha, Kalinin subunit alpha, Laminin-5 subunit alpha, Laminin-6 subunit alpha, Laminin-7 subunit alpha, Nicein subunit alpha, E170

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Negative control :

Paraffin-embedded human lymph node tissue stained for LAMA3 using ab242197 at 1 μg/mL in immunohistochemcial analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Paraffin-embedded human colon tissue stained for LAMA3 using ab242197 at 1 μg/mL in immunohistochemcial analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Paraffin-embedded human stomach tissue stained for LAMA3 using ab242197 at 1 μg/mL in immunohistochemcial analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Paraffin-embedded human oral mucosa tissue stained for LAMA3 using ab242197 at 1 μg/mL in immunohistochemcial analysis.

Supplier Data

Western blot - Anti-LAMA3 antibody [CL3112] (AB242197)

All lanes:

Western blot - Anti-LAMA3 antibody [CL3112] (ab242197) at 1 µg/mL

Lane 1:

Purified human Lamin-332 recombinant protein

Lane 2:

Purified human Lamin-421 recombinant protein

Lane 3:

Purified human Lamin-511 recombinant protein

Lane 4:

Purified human Lamin-121 recombinant protein

Lane 5:

Purified human Lamin-221 recombinant protein

Predicted band size: 367 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3112

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human LAMA3 aa 2000-2150. The exact immunogen used to generate this antibody is proprietary information.

Q16787

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin subunit alpha-3 also known by the gene name LAMA3 is a critical component of the extracellular matrix. The protein has a molecular weight of approximately 400 kDa. LAMA3 is primarily expressed in epithelial tissues contributing significantly to the structural integrity of basement membranes. The protein often partners with other laminin subunits to form different laminin isoforms which are essential for cellular adhesion and signaling.

Biological function summary

LAMA3 plays an important role in the formation of stable basement membranes and interacts with other proteins to maintain tissue architecture. As part of the laminin-332 complex alongside beta and gamma laminin chains LAMA3 ensures cell adhesion migration and differentiation processes. Its expression in tissues like skin and mucosal surfaces highlights its function in providing structural support and cellular microenvironment regulation.

Pathways

Laminin subunit alpha-3 is essential in pathways related to cell adhesion and migration such as integrin signaling and epithelial-mesenchymal transition. These pathways are pivotal during wound healing and tissue regeneration. LAMA3 associates with integrins particularly beta-1 and beta-4 integrins facilitating communication between cells and their extracellular environment. The interaction with integrins enables cells to respond to structural changes and external stimuli.

Mutations or alterations in LAMA3 can lead to conditions such as Epidermolysis Bullosa and Junctional Epidermolysis Bullosa where the integrity of the skin and epithelial tissues is compromised. Laminin subunit gamma-2 often interacts with LAMA3 and anomalies in this partnership contribute to these severe blistering disorders. Understanding the link between LAMA3 and these diseases helps inform the development of targeted therapies aimed at restoring normal cell-matrix interactions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.. Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.

See full target information LAMA3

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 26:187 PubMed39707454

2024

Cilengitide sensitivity is predicted by overall integrin expression in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nomeda Girnius,Aylin Z Henstridge,Benjamin Marks,Jeffrey K Yu,G Kenneth Gray,Chris Sander,Ioannis K Zervantonakis,Augustin Luna

Heliyon 10:e36234 PubMed39253230

2024

Comprehensive analysis and experimental verification of the mechanism of anoikis related genes in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Bao,Dongqian Li,Xinyu Yang,Shiqi Ren,Haoxiang Ding,Chengfeng Guo,Jian Wan,Yicheng Xiong,MingYan Zhu,Yao Wang

Analytical cellular pathology (Amsterdam) 2024:8810804 PubMed38826849

2024

FAP Serves as a Prognostic Biomarker in Head and Neck Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanpeng Liao,Haidong Fan,Junquan Weng,Jieyu Zhou,Yuyan Zheng

Radiology and oncology 58:234-242 PubMed38452390

2024

Correlation of laminin subunit alpha 3 expression in pancreatic ductal adenocarcinoma with tumor liver metastasis and survival.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyi Xing,Xue Jing,Gong Qing,Yueping Jiang

Journal of ovarian research 14:67 PubMed33992120

2021

LAMA3 DNA methylation and transcriptome changes associated with chemotherapy resistance in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Yuan Feng,Yong-Zhi Huang,Wei Zhang,Li Li

Stem cells (Dayton, Ohio) 39:897-903 PubMed33609408

2021

ABCB5+ dermal mesenchymal stromal cells with favorable skin homing and local immunomodulation for recessive dystrophic epidermolysis bullosa treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Riedl,Michael Pickett-Leonard,Cindy Eide,Mark Andreas Kluth,Christoph Ganss,Natasha Y Frank,Markus H Frank,Christen L Ebens,Jakub Tolar

Molecular therapy. Nucleic acids 18:166-182 PubMed31557618

2019

Long Non-coding RNA LINC00628 Interacts Epigenetically with the LAMA3 Promoter and Contributes to Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Feng Xu,Yue Zheng,Ling Zhang,Ping Wang,Chun-Mi Niu,Tong Wu,Qi Tian,Xiao-Bo Yin,Shan-Shan Shi,Lei Zheng,Li-Ming Gao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAMA3 antibody [CL3112]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [CL3112] (AB242197)

Western blot - Anti-LAMA3 antibody [CL3112] (AB242197)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise