Anti-LAMA3 antibody [EPR28286-78]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [EPR28286-78] (AB321976)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling LAMA3 with ab321976 at 1/100 (4.88 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human spleen.
The section was incubated with ab321976 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [EPR28286-78] (AB321976)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling LAMA3 with ab321976 at 1/100 (4.88 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in basement membrane of human colon.
The section was incubated with ab321976 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [EPR28286-78] (AB321976)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LAMA3 with ab321976 at 1/100 (4.88 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human liver.
The section was incubated with ab321976 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-LAMA3 antibody [EPR28286-78] (AB321976)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human esophagus tissue staining Eph receptor A2 with ab324955 at a 1/50 (10.12 μg/ml) dilution, ab316099 anti-COL4A2 used at a 1/500 (1.07 μg/ml) dilution and ab321976 anti-LAMA3 used at a 1/100 (4.88 μg/ml) dilution.

Panel A : anti-Eph receptor A2 (green; Opal™520), anti-COL4A2 (magenta; Opal™690), anti-LAMA3 (gray; Opal™570) on human esophagus.

Panel B : anti-Eph receptor A2 staining epithelium in human esophagus.

Panel C : anti-COL4A2 staining endothelium and smooth muscle in human esophagus.

Panel D : anti-LAMA3 staining basement membrane in human esophagus.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324955, ab316099 and ab321976 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [EPR28286-78] (AB321976)

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling LAMA3 with ab321976 at 1/100 (4.88 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in basement membrane of human endometrial carcinoma.
The section was incubated with ab321976 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA3 antibody [EPR28286-78] (AB321976)

Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling LAMA3 with ab321976 at 1/100 (4.88 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in basement membrane of human skin.
The section was incubated with ab321976 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• Dot

Supplier Data

Dot Blot - Anti-LAMA3 antibody [EPR28286-78] (AB321976)

Dot blot analysis of LAMA3 using ab321976 at 1 : 1000 (0.488 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged human LAMA3 fragment
Lane2 : His-tagged human LAMA4 fragment
Lane3 : His-tagged human LAMA5 fragment
Lane4 : His-tagged human LAMA1 fragment
Lane5 : His-tagged human LAMA2 fragment

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human LAMA4, LAMA5, LAMA1 and LAMA2.

#In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-LAMA3 antibody [EPR28286-78] (ab321976) at 1/1000 dilution

Lane 1:

His-tagged human LAMA3 fragment

Lane 2:

His-tagged human LAMA4 fragment

Lane 3:

His-tagged human LAMA5 fragment

Lane 4:

His-tagged human LAMA1 fragment

Lane 5:

His-tagged human LAMA2 fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s