JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB151715

Anti-LAMA3 antibody [EPR8266]

Be the first to review this product! Submit a review

|

(8 Publications)

Rabbit Recombinant Monoclonal LAMA3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications.

View Alternative Names

LAMNA, LAMA3, Laminin subunit alpha-3, Epiligrin 170 kDa subunit, Epiligrin subunit alpha, Kalinin subunit alpha, Laminin-5 subunit alpha, Laminin-6 subunit alpha, Laminin-7 subunit alpha, Nicein subunit alpha, E170

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-LAMA3 antibody [EPR8266] (AB151715)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LAMA3 antibody [EPR8266] (AB151715)

Immunofluorescent analysis of HepG2 cells labeling LAMA3 with ab151715 at 1/100 dilution.

Western blot - Anti-LAMA3 antibody [EPR8266] (AB151715)
  • WB

Unknown

Western blot - Anti-LAMA3 antibody [EPR8266] (AB151715)

All lanes:

Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

Human skin lysate at 10 µg

Lane 5:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 367 kDa

false

Western blot - Anti-LAMA3 antibody [EPR8266] (AB151715)
  • WB

Lab

Western blot - Anti-LAMA3 antibody [EPR8266] (AB151715)

Lanes 1 - 3 : Merged signal (red and green). Green - ab151715 observed at 367 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab151715 was shown to react with LAMA3 in wild-type HeLa cells in western blot with loss of signal observed in LAMA3 knockout cell line ab265663 (LAMA3 knockout cell lysate ab257497). Wild-type and LAMA3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab151715 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

LAMA3 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human LAMA3 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-lama3-knockout-hela-cell-line-ab265663'>ab265663</a>)

Lane 3:

A431 cell lysate at 20 µg

Predicted band size: 367 kDa

Observed band size: 367 kDa

false

Western blot - Anti-LAMA3 antibody [EPR8266] (AB151715)
  • WB

Lab

Western blot - Anti-LAMA3 antibody [EPR8266] (AB151715)

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-LAMA3 antibody [EPR8266] (ab151715) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 1:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 367 kDa

true

Exposure time: 100s

  • Carrier free

    Anti-LAMA3 antibody [EPR8266] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8266

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB150385

Anti-LAMB3 antibody [EPR7525]

RabMAb|Recombinant

Western blot - Anti-LAMB3 antibody [EPR7525] (AB150385)

  • 0
  • 0
Primary Antibodies

AB274376

Anti-LAMC2 antibody [EPR23654-127]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-LAMC2 antibody [EPR23654-127] (AB274376)

  • 5
  • 1
Primary Antibodies

AB11575

Anti-Laminin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)

  • 5
  • 73
Primary Antibodies

AB78286

Anti-Laminin 5 antibody [P3H9-2]

BOND RX™ Validated|Trial Size

Immunohistochemistry (Frozen sections) - Anti-Laminin 5 antibody [P3H9-2] (AB78286)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin subunit alpha-3 also known by the gene name LAMA3 is a critical component of the extracellular matrix. The protein has a molecular weight of approximately 400 kDa. LAMA3 is primarily expressed in epithelial tissues contributing significantly to the structural integrity of basement membranes. The protein often partners with other laminin subunits to form different laminin isoforms which are essential for cellular adhesion and signaling.
Biological function summary

LAMA3 plays an important role in the formation of stable basement membranes and interacts with other proteins to maintain tissue architecture. As part of the laminin-332 complex alongside beta and gamma laminin chains LAMA3 ensures cell adhesion migration and differentiation processes. Its expression in tissues like skin and mucosal surfaces highlights its function in providing structural support and cellular microenvironment regulation.

Pathways

Laminin subunit alpha-3 is essential in pathways related to cell adhesion and migration such as integrin signaling and epithelial-mesenchymal transition. These pathways are pivotal during wound healing and tissue regeneration. LAMA3 associates with integrins particularly beta-1 and beta-4 integrins facilitating communication between cells and their extracellular environment. The interaction with integrins enables cells to respond to structural changes and external stimuli.

Mutations or alterations in LAMA3 can lead to conditions such as Epidermolysis Bullosa and Junctional Epidermolysis Bullosa where the integrity of the skin and epithelial tissues is compromised. Laminin subunit gamma-2 often interacts with LAMA3 and anomalies in this partnership contribute to these severe blistering disorders. Understanding the link between LAMA3 and these diseases helps inform the development of targeted therapies aimed at restoring normal cell-matrix interactions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.. Laminin-5 is thought to be involved in (1) cell adhesion via integrin alpha-3/beta-1 in focal adhesion and integrin alpha-6/beta-4 in hemidesmosomes, (2) signal transduction via tyrosine phosphorylation of pp125-FAK and p80, (3) differentiation of keratinocytes.
See full target information LAMA3
style
left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 44:4745-4771 PubMed40707625

2025

ALS-associated RNA-binding proteins promote UNC13A transcription through REST downregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuaki Watanabe,Naoki Suzuki,Tadashi Nakagawa,Masaki Hosogane,Tetsuya Akiyama,Naotoshi Kageyama,Yukino Funayama,Hitoshi Warita,Satoru Morimoto,Hideyuki Okano,Masashi Aoki,Keiko Nakayama

Communications biology 8:394 PubMed40057671

2025

Single-cell analysis reveals transcriptomic features and therapeutic targets in primary pulmonary lymphoepithelioma-like carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Binghua Tan,Ke Xu,Yingcheng Lyu,Yicheng Liang,Ruihao Liang,Kai Lei,Jialu Liang,Jing Huang,Kefeng Wang,Duoguang Wu,Wenjian Wang,Xueting Hu,Kexi Wang,Minghui Wang,Huayue Lin

iScience 27:109888 PubMed38947517

2024

Secretion of WNT7A by UC-MSCs assist in promoting the endometrial epithelial regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Fangbo Liu,Qin Lin,Shaolei Shen,Zhihong Li,Xiaorui Xie,Quan Cheng,Lan Wang,Yin Long,Juan Wang,Li Liu

NPJ biofilms and microbiomes 10:39 PubMed38589501

2024

Integrative analysis reveals associations between oral microbiota dysbiosis and host genetic and epigenetic aberrations in oral cavity squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liuyang Cai,Hengyan Zhu,Qianqian Mou,Po Yee Wong,Linlin Lan,Cherrie W K Ng,Pu Lei,Man Kit Cheung,Daijuanru Wang,Eddy W Y Wong,Eric H L Lau,Zenon W C Yeung,Ronald Lai,Katie Meehan,Sherwood Fung,Kwan Chee A Chan,Vivian W Y Lui,Alfred S L Cheng,Jun Yu,Paul K S Chan,Jason Y K Chan,Zigui Chen

Molecular medicine reports 26: PubMed36043531

2022

Palmitoyl‑RGD promotes the expression of dermal‑epidermal junction components in HaCaT cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joo Hyuck Lim,Jung Soo Bae,Seung Ki Lee,Dong Hun Lee

Molecular cell 82:2900-2911.e7 PubMed35905735

2022

Mapping subcellular localizations of unannotated microproteins and alternative proteins with MicroID.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenkun Na,Xiaoyun Dai,Shu-Jian Zheng,Carson J Bryant,Ken H Loh,Haomiao Su,Yang Luo,Amber F Buhagiar,Xiongwen Cao,Susan J Baserga,Sidi Chen,Sarah A Slavoff

Molecular medicine reports 23: PubMed33179081

2020

Laminin‑332 mediates proliferation, apoptosis, invasion, migration and epithelial‑to‑mesenchymal transition in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Caiqun Huang,Jun Chen

Journal of autoimmunity 114:102500 PubMed32565048

2020

Alterations in corneal biomechanics underlie early stages of autoimmune-mediated dry eye disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yael Efraim,Feeling Yu Ting Chen,Connor Stashko,Ka Neng Cheong,Eliza Gaylord,Nancy McNamara,Sarah M Knox
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com