Anti-LAMA3 antibody [EPR8266] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (AB249033)

This data was developed using ab151715, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of HepG2 cells labeling LAMA3 with ab151715 at 1/100 dilution.

• WB

Lab

Western blot - Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (AB249033)

This data was developed using the same antibody clone in a different buffer formulation (ab151715).

Lanes 1 - 3 : Merged signal (red and green). Green - ab151715 observed at 367 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab151715 was shown to react with LAMA3 in wild-type HeLa cells in western blot with loss of signal observed in LAMA3 knockout cell line ab265663 (LAMA3 knockout cell lysate ab257497). Wild-type and LAMA3 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab151715 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LAMA3 antibody [EPR8266] (<a href='/en-us/products/primary-antibodies/lama3-antibody-epr8266-ab151715'>ab151715</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

LAMA3 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human LAMA3 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-lama3-knockout-hela-cell-line-ab265663'>ab265663</a>)

Lane 3:

A431 cell lysate at 20 µg

Predicted band size: 367 kDa

Observed band size: 367 kDa

false

• WB

Lab

Western blot - Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (AB249033)

This data was developed using ab151715, the same antibody clone in a different buffer formulation.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-LAMA3 antibody [EPR8266] (<a href='/en-us/products/primary-antibodies/lama3-antibody-epr8266-ab151715'>ab151715</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 1:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 367 kDa

true

Exposure time: 100s

• WB

Unknown

Western blot - Anti-LAMA3 antibody [EPR8266] - BSA and Azide free (AB249033)

This data was developed using ab151715, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LAMA3 antibody [EPR8266] (<a href='/en-us/products/primary-antibodies/lama3-antibody-epr8266-ab151715'>ab151715</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

Human skin lysate at 10 µg

Lane 5:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 367 kDa

false