AB327111

Anti-LAMB1 Antibody [CL2970]

Name: Anti-LAMB1 Antibody [CL2970] (ab327111) | Abcam
Brand: Abcam Limited
SKU: AB327111
Price: 580 USD
Availability: InStock

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Mouse Monoclonal LAMB1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LAMB1.

View Alternative Names

Laminin subunit beta-1, Laminin B1 chain, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, LAMB1

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB1 Antibody [CL2970] (AB327111)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB1 Antibody [CL2970] (AB327111)

Immunohistochemical staining of human endometrium using ab327111 at 1 : 50–1 : 200 dilution, showing immunoreactivity in basement membrane of glandular epithelium.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB1 Antibody [CL2970] (AB327111)

Immunohistochemical staining of human placenta using ab327111 at 1 : 50–1 : 200 dilution, showing moderate immunoreactivity in basement membrane of trophoblast and in endothelium.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB1 Antibody [CL2970] (AB327111)

Immunohistochemical staining of human pancreas using ab327111 at 1 : 50–1 : 200 dilution, showing moderate positivity in basement membrane of exocrine glands.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB1 Antibody [CL2970] (AB327111)

Immunohistochemical staining of human stomach using ab327111 at 1 : 50–1 : 200 dilution, showing positivity in basement membrane of glandular epithelium.

Supplier Data

Western blot - Anti-LAMB1 Antibody [CL2970] (AB327111)

Western blot analysis in human placenta tissue using ab327111 at 1 µg/ml.

All lanes:

Western blot - Anti-LAMB1 Antibody [CL2970] (ab327111)

All lanes:

Human placenta tissue

Predicted band size: 198.04 kDa

false

Supplier Data

Western blot - Anti-LAMB1 Antibody [CL2970] (AB327111)

Western blot analysis of purified human recombinant Laminin-111, Laminin-211, Laminin-411, Laminin-511, Laminin-521 and Laminin-332 using ab327111 at 1 µg/ml.

All lanes:

Western blot - Anti-LAMB1 Antibody [CL2970] (ab327111)

Lane 2:

Human recombinant Laminin-111

Lane 3:

Human recombinant Laminin-211

Lane 4:

Human recombinant Laminin-411

Lane 5:

Human recombinant Laminin-511

Lane 6:

Human recombinant Laminin-521

Lane 7:

Human recombinant Laminin-332

Predicted band size: 198.04 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB1 Antibody [CL2970] (AB327111)

Immunohistochemical staining of human lymph node using ab327111 at 1 : 50–1 : 200 dilution, showing absence of immunoreactivity in lymphoid cells (negative control).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2970

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LAMB1.

P07942

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of the cerebral cortex (PubMed : 23472759). It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons (By similarity). Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface (By similarity). As a subunit of laminin-1 (also known as laminin-111 or EHS laminin), it is involved in the stimulation of agrin-induced receptor clustering through a MuSK-independent pathway (By similarity).

See full target information LAMB1

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Product promise

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