AB327112

Anti-LAMB2 Antibody [CL2976]

Name: Anti-LAMB2 Antibody [CL2976] (ab327112) | Abcam
Brand: Abcam Limited
SKU: AB327112
Price: 600 USD
Availability: InStock

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Mouse Monoclonal Laminin beta 2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LAMB2.

View Alternative Names

LAMS, LAMB2, Laminin subunit beta-2, Laminin B1s chain, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, S-laminin subunit beta, S-LAM beta

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB2 Antibody [CL2976] (AB327112)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB2 Antibody [CL2976] (AB327112)

Immunohistochemical staining of human cortex using ab327112 at 1 : 5000–1 : 10000 dilution, showing strong immunoreactivity in endothelial cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB2 Antibody [CL2976] (AB327112)

Immunohistochemical staining of human skeletal muscle using ab327112 at 1 : 5000–1 : 10000 dilution, showing strong membranous immunoreactivity in myocytes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB2 Antibody [CL2976] (AB327112)

Immunohistochemical staining of human colon using ab327112 at 1 : 5000–1 : 10000 dilution, showing strong positivity in basement membrane of glandular epithelium.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB2 Antibody [CL2976] (AB327112)

Immunohistochemical staining of human heart using ab327112 at 1 : 5000–1 : 10000 dilution, showing strong membranous immunoreactivity in cardiomyocytes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB2 Antibody [CL2976] (AB327112)

Immunohistochemical staining of human kidney using ab327112 at 1 : 5000–1 : 10000 dilution, showing strong positivity in renal glomeruli.

Supplier Data

Western blot - Anti-LAMB2 Antibody [CL2976] (AB327112)

Western blot analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using ab327112 at 1 µg/ml. Remaining relative intensity is presented. Loading control : Anti-GAPDH.

All lanes:

Western blot - Anti-LAMB2 Antibody [CL2976] (ab327112)

Lane 2:

U-138MG cells transfected with control siRNA

Lane 3:

U-138MG cells transfected with target specific siRNA probe #1

Lane 4:

U-138MG cells transfected with target specific siRNA probe #2

Predicted band size: 195.98 kDa

false

Supplier Data

Western blot - Anti-LAMB2 Antibody [CL2976] (AB327112)

Western blot analysis of purified human recombinant Laminin-421, Laminin-521, Laminin-332 and Laminin-111 using ab327112 at 1 µg/ml.

All lanes:

Western blot - Anti-LAMB2 Antibody [CL2976] (ab327112)

Lane 2:

purified human recombinant Laminin-421

Lane 3:

purified human recombinant Laminin-521

Lane 4:

purified human recombinant Laminin-322

Lane 5:

purified human recombinant Laminin-111

Predicted band size: 195.98 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB2 Antibody [CL2976] (AB327112)

Immunohistochemical staining of human lymph node using ab327112 at 1 : 5000–1 : 10000 dilution, showing absence of immunoreactivity in lymphoid cells (negative control).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2976

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LAMB2.

P55268

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

See full target information LAMB2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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