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AB97765

Anti-LAMB3 antibody

5

(1 Review)

|

(6 Publications)

Rabbit Polyclonal LAMB3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LAMB3 aa 600-950.

View Alternative Names

LAMNB1, LAMB3, Laminin subunit beta-3, Epiligrin subunit beta, Kalinin B1 chain, Kalinin subunit beta, Laminin B1k chain, Laminin-5 subunit beta, Nicein subunit beta

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 antibody (AB97765)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 antibody (AB97765)

Immunohistochemical analysis of paraffin-embedded human lung cancer, using ab97765 to detect LAMB3 at 1 : 250.

Immunocytochemistry/ Immunofluorescence - Anti-LAMB3 antibody (AB97765)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAMB3 antibody (AB97765)

Immunofluorescence analysis of methanol-fixed HeLa, using ab97765 at 1 : 200.

Western blot - Anti-LAMB3 antibody (AB97765)
  • WB

Unknown

Western blot - Anti-LAMB3 antibody (AB97765)

7.5% SDS PAGE

All lanes:

Western blot - Anti-LAMB3 antibody (ab97765) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 130 kDa

false

Western blot - Anti-LAMB3 antibody (AB97765)
  • WB

Unknown

Western blot - Anti-LAMB3 antibody (AB97765)

7.5% SDS PAGE

All lanes:

Western blot - Anti-LAMB3 antibody (ab97765) at 1/1000 dilution

All lanes:

NIH-3T3 whole cell lysate at 30 µg

Predicted band size: 130 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human LAMB3 aa 600-950. The exact immunogen used to generate this antibody is proprietary information.

Q13751

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Alternative Products

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Primary Antibodies

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Complementary Products

Primary Antibodies

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  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Anti-Laminin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (AB11575)

  • 5
  • 74
Primary Antibodies

AB182120

Anti-Integrin beta 4 antibody [EPR17517]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 4 antibody [EPR17517] (AB182120)

  • 5
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin subunit beta-3 (LAMB3) is a protein that is part of the laminin family commonly referred to as Laminin-332 or Laminin-5. It weighs approximately 117 kDa. LAMB3 is expressed in the epithelial basement membranes of various tissues including skin and mucous membranes. The protein forms a network essential for maintaining the structural integrity of these membranes playing a mechanical role in cellular adhesion and migration.
Biological function summary

LAMB3 functions within a heterotrimeric complex that includes Laminin-3 (alpha subunit) and Laminin-3 (gamma subunit). These interactions lead to the formation of Laminin-332 which serves as a backbone for cell attachment and spreading. It is important in processes such as epithelial cell proliferation and wound healing. By stabilizing attachments between the basement membrane and epithelial cells LAMB3 supports tissue integrity and repair mechanisms.

Pathways

LAMB3 plays a role in both the integrin signaling and epidermal growth factor receptor (EGFR) pathways. It connects with proteins like the integrin alpha-6 beta-4 complex facilitating cell adhesion and communication with the extracellular matrix. In the EGFR pathway LAMB3's interactions enhance signaling that promotes cellular proliferation and survival playing a part in skin development and maintenance.

Mutations or dysregulations in LAMB3 relate notably to junctional epidermolysis bullosa and certain cancers like squamous cell carcinoma. In junctional epidermolysis bullosa the protein loses its adhesive function affecting the epithelial junctions and causing severe skin blistering. LAMB3 also associates with integrin proteins in cancer contributing to tumor progression and metastasis when dysfunctional affecting cell adhesion and integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
See full target information LAMB3
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of oral science 17:29 PubMed40240323

2025

Programmed death-ligand 1 regulates ameloblastoma growth and recurrence.

Applications

Unspecified application

Species

Unspecified reactive species

Linzhou Zhang,Hao Lin,Jiajie Liang,Xuanhao Liu,Chenxi Zhang,Qiwen Man,Ruifang Li,Yi Zhao,Bing Liu

Aging 16:7902-7914 PubMed38709270

2024

Improvement of skin wound healing by giant salamander skin mucus gel wrapped with bone marrow mesenchymal stem cells via affecting integrin family molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Dayong Du,Yan Huang,Cui Xu,Yang Liu,Xiaohong Wu,Jie Yang,Zhipeng Liu,Jianxin Ma,Chaoji Huangfu

International journal of molecular sciences 25: PubMed38473784

2024

Downregulation of LAMB3 Altered the Carcinogenic Properties of Human Papillomavirus 16-Positive Cervical Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Warattaya Wattanathavorn,Masahide Seki,Yutaka Suzuki,Supranee Buranapraditkun,Nakarin Kitkumthorn,Thanayod Sasivimolrattana,Parvapan Bhattarakosol,Arkom Chaiwongkot

Frontiers in oncology 10:553983 PubMed33194615

2020

Human Endogenous Retrovirus Expression Is Upregulated in the Breast Cancer Microenvironment of HIV Infected Women: A Pilot Study.

Applications

Unspecified application

Species

Unspecified reactive species

Gislaine Curty,Greta A Beckerle,Luis P Iñiguez,Robert L Furler,Pedro S de Carvalho,Jez L Marston,Stephane Champiat,Jonas J Heymann,Christopher E Ormsby,Gustavo Reyes-Terán,Marcelo A Soares,Douglas F Nixon,Matthew L Bendall,Fabio E Leal,Miguel de Mulder Rougvie

Skin pharmacology and physiology 33:110-118 PubMed32570235

2020

Vascular Endothelial Growth Factor Blockade Induces Dermal Endothelial Cell Apoptosis in a Clinically Relevant Skin Organ Culture Model.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Luengas-Martinez,Jonathan Hardman-Smart,David Rutkowski,Talveen S Purba,Ralf Paus,Helen S Young

Cancer biotherapy & radiopharmaceuticals 35:319-328 PubMed32412777

2020

LncRNA Promotes Bladder Cancer Occurrence Via Protecting from Downregulation by Sponging .

Applications

Unspecified application

Species

Unspecified reactive species

Dong Chen,Junyi Chen,Jialin Gao,Yongrui Zhang,Yongzhi Ma,Wei Wei,Yong Wei
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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