AB327113

Anti-LAMB3 Antibody [CL3353 ]

Name: Anti-LAMB3 Antibody [CL3353 ] (ab327113) | Abcam
Brand: Abcam Limited
SKU: AB327113
Price: 580 USD
Availability: InStock

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Mouse Monoclonal LAMB3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human LAMB3.

View Alternative Names

LAMNB1, LAMB3, Laminin subunit beta-3, Epiligrin subunit beta, Kalinin B1 chain, Kalinin subunit beta, Laminin B1k chain, Laminin-5 subunit beta, Nicein subunit beta

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

Immunohistochemical staining of human prostate using ab327113 at 1 : 500–1 : 1000 dilution, showing moderate to strong positivity in basement membrane of glandular cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

Immunohistochemical staining of human fallopian tube using ab327113 at 1 : 500–1 : 1000 dilution, showing moderate to strong positivity in basement membrane of glandular cells.

Immunocytochemistry/ Immunofluorescence - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

Immunofluorescence staining of A-431 cells using ab327113 at 2–10 µg/ml, showing specific staining in the endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

Immunohistochemical staining of human skin using ab327113 at 1 : 500–1 : 1000 dilution, showing moderate positivity in the basement membrane of the basal layer of epidermal cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

Immunohistochemistry analysis in human skin and pancreas tissues using ab327113 at 1 : 500–1 : 1000 dilution. Corresponding LAMB3 RNA-seq data are presented for the same tissues.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

Immunohistochemical staining of rat rectum using ab327113 at 1 : 500–1 : 1000 dilution, showing moderate positivity in basement membrane of glandular cells, as well as strong positivity in endothelial cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

Immunohistochemical staining of rat kidney using ab327113 at 1 : 500–1 : 1000 dilution, showing strong positivity in basement membrane in cells in glomeruli.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

Immunohistochemical staining of rat skin using ab327113 at 1 : 500–1 : 1000 dilution, showing moderate to strong positivity in basement membrane basal epidermal cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

Immunohistochemical staining of rat lung using ab327113 at 1 : 500–1 : 1000 dilution, showing moderate to strong positivity in basement membrane of pneumocytes.

Supplier Data

Western blot - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

Western blot analysis in human lung tissue using ab327113 at 1 µg/ml.

All lanes:

Western blot - Anti-LAMB3 Antibody [CL3353 ] (ab327113)

All lanes:

Human lung tissue

Predicted band size: 129.57 kDa

false

Supplier Data

Western blot - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

Western blot analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221 using ab327113 at 1 µg/ml.

All lanes:

Western blot - Anti-LAMB3 Antibody [CL3353 ] (ab327113)

Lane 2:

Human recombinant Laminin-332

Lane 3:

Human recombinant Laminin-421

Lane 4:

Human recombinant Laminin-511

Lane 5:

Human recombinant Laminin-121

Lane 6:

Human recombinant Laminin-221

Predicted band size: 129.57 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3353 ] (AB327113)

Immunohistochemical staining of human pancreas using ab327113 at 1 : 500–1 : 1000 dilution, showing no positivity in glandular cells as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3353

Isotype

IgG1

Carrier free

Reacts with

Human, Rat

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LAMB3.

Q13751

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

See full target information LAMB3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com