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AB327114

Anti-LAMB3 Antibody [CL3363]

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Mouse Monoclonal LAMB3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LAMB3.

View Alternative Names

LAMNB1, LAMB3, Laminin subunit beta-3, Epiligrin subunit beta, Kalinin B1 chain, Kalinin subunit beta, Laminin B1k chain, Laminin-5 subunit beta, Nicein subunit beta

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3363] (AB327114)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3363] (AB327114)

Immunohistochemical staining of human fallopian tube using ab327114 at 1 : 200–1 : 500 dilution, showing strong positivity in basement membrane in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3363] (AB327114)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3363] (AB327114)

Immunohistochemical staining of human prostate using ab327114 at 1 : 200–1 : 500 dilution, showing moderate positivity in basement membrane in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3363] (AB327114)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3363] (AB327114)

Immunohistochemical staining of human duodenum using ab327114 at 1 : 200–1 : 500 dilution, showing moderate positivity in basement membrane in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3363] (AB327114)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3363] (AB327114)

Immunohistochemistry analysis in human duodenum and pancreas tissues using ab327114 at 1 : 200–1 : 500 dilution. Corresponding LAMB3 RNA-seq data are presented for the same tissues.

Western blot - Anti-LAMB3 Antibody [CL3363] (AB327114)
  • WB

Supplier Data

Western blot - Anti-LAMB3 Antibody [CL3363] (AB327114)

Western blot analysis of purified human recombinant Laminin-332, Laminin-421, Laminin-511, Laminin-121 and Laminin-221 using ab327114 at 1 µg/ml.

All lanes:

Western blot - Anti-LAMB3 Antibody [CL3363] (ab327114)

Lane 2:

Human recombinant Laminin-332

Lane 3:

Human recombinant Laminin-421

Lane 4:

Human recombinant Laminin-511

Lane 5:

Human recombinant Laminin-121

Lane 6:

Human recombinant Laminin-221

Predicted band size: 129.57 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3363] (AB327114)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMB3 Antibody [CL3363] (AB327114)

Immunohistochemical staining of human pancreas using ab327114 at 1 : 200–1 : 500 dilution, showing no positivity in exocrine glandular cells as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3363

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human LAMB3.

Q13751

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
See full target information LAMB3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com