AB128864

Anti-LAMB3 antibody [EPR7524]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal LAMB3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LAMNB1, LAMB3, Laminin subunit beta-3, Epiligrin subunit beta, Kalinin B1 chain, Kalinin subunit beta, Laminin B1k chain, Laminin-5 subunit beta, Nicein subunit beta

2 Images

Unknown

Western blot - Anti-LAMB3 antibody [EPR7524] (AB128864)

All lanes:

Western blot - Anti-LAMB3 antibody [EPR7524] (ab128864) at 1/1000 dilution

Lane 1:

HACAT cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 130 kDa

Observed band size: 140 kDa

false

OI-RD Scanning - Anti-LAMB3 antibody [EPR7524] (AB128864)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-LAMB3 antibody [EPR7524] (AB128864)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-LAMB3 antibody [EPR7524] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7524

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Laminin subunit beta-3 (LAMB3) is a protein that is part of the laminin family commonly referred to as Laminin-332 or Laminin-5. It weighs approximately 117 kDa. LAMB3 is expressed in the epithelial basement membranes of various tissues including skin and mucous membranes. The protein forms a network essential for maintaining the structural integrity of these membranes playing a mechanical role in cellular adhesion and migration.

Biological function summary

LAMB3 functions within a heterotrimeric complex that includes Laminin-3 (alpha subunit) and Laminin-3 (gamma subunit). These interactions lead to the formation of Laminin-332 which serves as a backbone for cell attachment and spreading. It is important in processes such as epithelial cell proliferation and wound healing. By stabilizing attachments between the basement membrane and epithelial cells LAMB3 supports tissue integrity and repair mechanisms.

Pathways

LAMB3 plays a role in both the integrin signaling and epidermal growth factor receptor (EGFR) pathways. It connects with proteins like the integrin alpha-6 beta-4 complex facilitating cell adhesion and communication with the extracellular matrix. In the EGFR pathway LAMB3's interactions enhance signaling that promotes cellular proliferation and survival playing a part in skin development and maintenance.

Mutations or dysregulations in LAMB3 relate notably to junctional epidermolysis bullosa and certain cancers like squamous cell carcinoma. In junctional epidermolysis bullosa the protein loses its adhesive function affecting the epithelial junctions and causing severe skin blistering. LAMB3 also associates with integrin proteins in cancer contributing to tumor progression and metastasis when dysfunctional affecting cell adhesion and integrity.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

See full target information LAMB3

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 23:4495-505 PubMed23051733

2012

Establishing epithelial glandular polarity: interlinked roles for ARF6, Rac1, and the matrix microenvironment.

Applications

Unspecified application

Species

Dog

Christine L Monteleon,Alanna Sedgwick,Alyssa Hartsell,Michael Dai,Catherine Whittington,Sherry Voytik-Harbin,Crislyn D'Souza-Schorey

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAMB3 antibody [EPR7524]

Western blot - Anti-LAMB3 antibody [EPR7524] (AB128864)

OI-RD Scanning - Anti-LAMB3 antibody [EPR7524] (AB128864)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise